We narrowed to 771 results for: Vars

1. Making CRISPR Plasmids Using Fragmid

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   Blog Post

   ...Schematic depicting available destination vectors for various delivery methods, along with compatible inserts...

2. Design Tips for Prime Editing

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   Blog Post

   ...rereading many of the original papers reporting various prime editing tools. These papers are chock full...

3. CRISPR in the Clinic

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   Blog Post

   ...transthyretin amyloidosis (hATTR) hATTR is caused by a variety of single nucleotide polymorphisms (SNPs) in the...

4. Viral Vectors 101: Virus Safety

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   Blog Post

   ...accident-prone researchers. Note that safety policies will vary slightly from organization to organization, so you...

5. Synthetic promoter AAVs for cell-type specific expression in retinal cells

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   Blog Post

   ...Esposti, F., Cowan, C.S., Bharioke, A., Patino-Alvarez, C.P., Keleş, Ö., Kusnyerik, A., Azoulay, T., Hartl...

6. Adapting Toehold Switches to Detect Zika Virus

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   Blog Post

   ...Jim Collins and Peng Yin at the Wyss Institute (Harvard University), we developed two new enabling technologies...

7. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

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   Blog Post

   ...that worked with NGS data were: Crispresso CrispRVariants AmpliCan We chose to adapt Crispresso by Luca...

8. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

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   Blog Post

   ...training from Massachusetts General Hospital and Harvard Medical School. Connect with her on LinkedIn @ ...

9. Tips for a 1st time AAV user (by a Rookie AAV user)

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   Blog Post

   ...MOI optimization to account for batch-to-batch variability. Check out this post to learn more about different...

10. Plasmids 101: 5 factors to help you choose the right cloning method

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    Blog Post

    ...versatile and efficient way to generate a wide variety of constructs. Similar, Gateway cloning (discussed...

11. 3D Printing Meets CRISPR Cas9

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    Blog Post

    ... this interrogation process, they will almost invariably say something like “Oh,…I didn’t quite appreciate...

12. Fluorescent Tagging of Endogenous Genes with SapTrap

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    Blog Post

    ...for easy screening of the insertion event, and a variety of connector modules (linker sequences between ...

13. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

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    Blog Post

    ...nuclease-induced gene disruption frequencies in the various cell types ranged from 18% to 65% (Maggio et al...

14. Transferable Skills: Negotiation

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    Blog Post

    ...researchers feel they lack formal training in a variety of transferable skills. At Addgene, we've set out...

15. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

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    Blog Post

    ...sometimes limited across different targets due to variations in chromatin context. Our recent studies (Mathis...

16. Antibodies 101: Flow Compensation

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    Blog Post

    ... research technician in Arlene Sharpe's lab at Harvard Medical School.   More resources on the Addgene...

17. Degrading DNA with Cascade-Cas3

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    Blog Post

    ...Cascade for maximum efficiency of the process. Various labs have designed all-in-one Cascade plasmids ...

18. PhD Applications After COVID

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    Blog Post

    ...are unable to attend in-person interviews for a variety of reasons (logistical, financial, etc…) now have...

19. Hot Plasmids and Viral Preps - May 2021

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    Blog Post

    ... Various Addgenies...

20. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

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    Blog Post

    ...expression. They found that the cellular fitness cost varied greatly between chromoproteins. In some cases, ...