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1. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

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   Blog Post

   ...details below) can also be adapted to a competitive format. Figure 2: Overview of using Competitive...the experiment by diluting your standard curve, performing a serial dilution of your experimental sample...

2. Hot Plasmids - October 2020

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   Blog Post

   ...time of synthesis and, after a few hours, a conformational change causes the protein to fluoresce red....lentiviral preps in addition to the pooled library format! The library inhibits over 18,000 human genes. ...

3. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

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   Blog Post

   ... all three edits and were thus more efficient. Informed by these mutations and previously described TadA...98.3%, or 98.6% efficiency, respectively) and outperformed ABE7.10 by at least 1.4 fold. The ability of...

4. Making CRISPR Plasmids Using Fragmid

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   Blog Post

   ...vector via Golden Gate cloning, perform an exonuclease cleanup, transform, plate, and pick. In short, the...

5. Hot Plasmids February 2024

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   ...whose FocalPlanes piece on mStayGold inspired and informed this section.  Find mStayGold plasmids at Addgene... (Chuapoco et al., 2023). They found the best-performing variant, CAP-Mac, was effective at breaching ...

6. Engaging with science and society at pgEd

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   Blog Post

   ...crucial if we want to make sure that everyone can be informed and empowered to make their own decisions about...social media and blog posts, lesson plans, or information briefs for policymakers. The rapidly changing...

7. Plasmids 101: Terminators and PolyA signals

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   Blog Post

   ...as intrinsic termination, and it relies on the formation of a GC-rich hairpin in the RNA transcript followed... for efficient rabbit beta-globin mRNA 3' end formation. Cell. 1987. PMID: 3568131.  Hager S, Frame FM...

8. The Materials Science of Optogenetics Experiments

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   Blog Post

   ...published first. Because behavioral optogenetics is performed in freely moving animals, the fiber optic patch...into some of the important considerations when performing optogenetics behavioral experiments. Thank you...

9. Transferable Skills: Negotiation

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   Blog Post

   ...survey found that many researchers feel they lack formal training in a variety of transferable skills. At...easily work on this transferable skill without any formal training or coursework. First, you have to develop...

10. xCas9: Engineering a CRISPR Variant with PAM Flexibility

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    Blog Post

    ... NGA is a secondary PAM for SpCas9. Hu et al. performed GUIDE-seq analysis to characterize genome-wide...an NGG PAM, xCas9 3.7-VPR constructs modestly outperformed SpCas9-VPR in activating transcription. Sites...

11. Addgene's Cancer Collection Pages Connect the Research Community

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    Blog Post

    ...growth and proliferation, while tumor suppressors perform the opposite task, keeping cells from dividing ...set of vectors for use with the Gateway Cloning Platform (Life Technologies, Carlsbad, CA) that represents...

12. Fluorescent Tagging of Endogenous Genes with SapTrap

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    Blog Post

    ...have its limitations, especially when trying to perform high-throughput experiments. If a user wants to...Allen Institute’s recent blog post. For more information on other CRISPR/Cas systems used for endogenous...

13. Droplet Digital PCR for AAV Quantitation

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    Blog Post

    ...of droplets) can be sufficient to capture the information needed for your PCR experiment. The process to...Furuta-Hanawa et al., 2019). Tips and tricks for performing ddPCR Clean everything You must have a clean ...

14. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

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    Blog Post

    ...list will let you jump directly to the type of information you are looking for in our catalog or educational...a simple search. These pages provide helpful information about the subject, link to related guides and...

15. Great Results Start with Great Standard Curves

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    Blog Post

    ...the standards versus the response and use that information to extrapolate the concentration of an unknown...generated by replicate values provides useful information about the accuracy of the curve, which we will...

16. New CRISPR Tools: Cas7-11 and PASTE

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    Blog Post

    ...target. In addition, when they looked at the transformed E. coli expressing Cas7-11, they found no evidence...system as Class I, Type III-E, and then used this information to identify a large variety of related bacteria...

17. An Inside Look into Addgene's Dev Team

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    Blog Post

    ... these steps, we discover we need additional information. It is quite possible that the clarifications...hows and whys of our users, we are able to make informed software design choices, resulting in software...

18. Supporting Reproducibility with a Connected ELN

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    Blog Post

    ...documents into RSpace, maintaining the original formatting. In this way data is moved from a static and ...conversations and import them into RSpace. The kind of informal conversations about research that can take place...

19. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

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    Blog Post

    ...difficult to obtain. These can then be used to perform cell therapy and to model diseases in vitro. Find...et al., Promotion of direct reprogramming by transformation-deficient Myc. Proc Natl Acad Sci U S A, 2010...

20. Career Insights: Technical Support Specialist

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    Blog Post

    ...resources and databases are scrutinized, and this information is enriched with your technical knowledge and...data sets, troubleshooting protocols, analyzing information, and solving customer problems. Technical support...