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Showing: 681 - 700 of 963 results
  1. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    ...interactions with RaPID RaPID is similar to the BioID platform for identifying protein-protein interactions, ...proteins reported by other research groups that performed BirA*-based affinity purification mass spec experiments...
  2. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...similar rates to untreated controls. Ma et al. performed sequencing of 23 potential off-target editing ...advancement in our understanding of how CRISPR will perform in embryos but also shows that many more experiments...
  3. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    ...the very nature of the way the experiments were performed, they did not see anything unique to male stem...reporting the sex of cells and tissues—when that information is reliable—is important to ensuring excellence...
  4. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    ...IIS sites and will be re-digested. In contrast, formation of the desired ligation product is irreversible...enzyme recognition sites after ligation favors formation of the construct of interest. Although efficiency...
  5. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    ...details below) can also be adapted to a competitive format. Figure 2: Overview of using Competitive...the experiment by diluting your standard curve, performing a serial dilution of your experimental sample...
  6. Hot Plasmids - October 2020

    Type
    Blog Post
    ...time of synthesis and, after a few hours, a conformational change causes the protein to fluoresce red....lentiviral preps in addition to the pooled library format! The library inhibits over 18,000 human genes. ...
  7. Hot Plasmids February 2024

    Type
    Blog Post
    ...whose FocalPlanes piece on mStayGold inspired and informed this section.  Find mStayGold plasmids at Addgene... (Chuapoco et al., 2023). They found the best-performing variant, CAP-Mac, was effective at breaching ...
  8. Engaging with science and society at pgEd

    Type
    Blog Post
    ...crucial if we want to make sure that everyone can be informed and empowered to make their own decisions about...social media and blog posts, lesson plans, or information briefs for policymakers. The rapidly changing...
  9. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...as intrinsic termination, and it relies on the formation of a GC-rich hairpin in the RNA transcript followed... for efficient rabbit beta-globin mRNA 3' end formation. Cell. 1987. PMID: 3568131.  Hager S, Frame FM...
  10. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    ...published first. Because behavioral optogenetics is performed in freely moving animals, the fiber optic patch...into some of the important considerations when performing optogenetics behavioral experiments. Thank you...
  11. Transferable Skills: Negotiation

    Type
    Blog Post
    ...survey found that many researchers feel they lack formal training in a variety of transferable skills. At...easily work on this transferable skill without any formal training or coursework. First, you have to develop...
  12. Hot Plasmids: Summer 2025

    Type
    Blog Post
    ...over mVenus and 4-fold over the previous best performer mGold — while maintaining high brightness and ...Emily P. Bentley While several viral tools can perform neuronal tracing, anterograde tracing poses challenges...
  13. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    ... NGA is a secondary PAM for SpCas9. Hu et al. performed GUIDE-seq analysis to characterize genome-wide...an NGG PAM, xCas9 3.7-VPR constructs modestly outperformed SpCas9-VPR in activating transcription. Sites...
  14. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    ...have its limitations, especially when trying to perform high-throughput experiments. If a user wants to...Allen Institute’s recent blog post. For more information on other CRISPR/Cas systems used for endogenous...
  15. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ...of droplets) can be sufficient to capture the information needed for your PCR experiment. The process to...Furuta-Hanawa et al., 2019). Tips and tricks for performing ddPCR Clean everything You must have a clean ...
  16. Great Results Start with Great Standard Curves

    Type
    Blog Post
    ...the standards versus the response and use that information to extrapolate the concentration of an unknown...generated by replicate values provides useful information about the accuracy of the curve, which we will...
Showing: 681 - 700 of 963 results