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Showing: 681 - 700 of 960 results

  1. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    ...details below) can also be adapted to a competitive format. Figure 2: Overview of using Competitive...the experiment by diluting your standard curve, performing a serial dilution of your experimental sample...

  3. Hot Plasmids February 2024

    Type
    Blog Post
    ...whose FocalPlanes piece on mStayGold inspired and informed this section.  Find mStayGold plasmids at Addgene... (Chuapoco et al., 2023). They found the best-performing variant, CAP-Mac, was effective at breaching ...

  4. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...as intrinsic termination, and it relies on the formation of a GC-rich hairpin in the RNA transcript followed... for efficient rabbit beta-globin mRNA 3' end formation. Cell. 1987. PMID: 3568131.  Hager S, Frame FM...

  5. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    ...published first. Because behavioral optogenetics is performed in freely moving animals, the fiber optic patch...into some of the important considerations when performing optogenetics behavioral experiments. Thank you...

  6. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    ... NGA is a secondary PAM for SpCas9. Hu et al. performed GUIDE-seq analysis to characterize genome-wide...an NGG PAM, xCas9 3.7-VPR constructs modestly outperformed SpCas9-VPR in activating transcription. Sites...

  7. Transferable Skills: Negotiation

    Type
    Blog Post
    ...survey found that many researchers feel they lack formal training in a variety of transferable skills. At...easily work on this transferable skill without any formal training or coursework. First, you have to develop...

  8. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    ...have its limitations, especially when trying to perform high-throughput experiments. If a user wants to...Allen Institute’s recent blog post. For more information on other CRISPR/Cas systems used for endogenous...

  10. Great Results Start with Great Standard Curves

    Type
    Blog Post
    ...the standards versus the response and use that information to extrapolate the concentration of an unknown...generated by replicate values provides useful information about the accuracy of the curve, which we will...

  11. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ...of droplets) can be sufficient to capture the information needed for your PCR experiment. The process to...Furuta-Hanawa et al., 2019). Tips and tricks for performing ddPCR Clean everything You must have a clean ...

  13. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ...left undisturbed for 13 days. (a, b, c) Colonies formed by expansion of single cells for 13 days. lentiCas9... of a stable cell culture after transduction. Performing antibiotic selection on transduced cells enables...5 dilutions plus one 'no virus' control well). Perform a "reverse transduction" by seeding 50,000 cells...untransduced well (0 µL lentivirus, above) are dying. Perform regular media changes and monitor the growth of...

  14. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ...inverting several times. A white precipitate will be formed which contains the bacterial proteins and genomic...Optional: Perform phenol-chloroform extraction - see protocol below . Note: Phenol-chloroform extraction...mL RNase A TE or water-saturated phenol-chloroform Chloroform 100% ethanol or isopropanol 90% ethanol ...Phenol-Chloroform Extraction of DNA Samples Add an equal volume of TE-saturated phenol-chloroform to the...volume of chloroform to the recovered aqueous DNA layer. Repeat steps 2-4. Note: Phenol-chloroform is a hazardous...purified without a kit it is advisable to perform a phenol/chloroform extraction of the supernatant after step...Bacterial Culture You may also like... Bacterial Transformation Agarose Gel Electrophoresis Agarose Gel DNA...

  15. What is Polymerase Chain Reaction (PCR)

    Type
    Protocol
    ...supplied by the buffer stock, where it is known to form a gradient when frozen. DMSO helps with denaturation...Digest of Plasmid DNA DNA Ligation Background Information A standard Polymerase Chain Reaction (PCR) is...Watch the protocol video below to learn how to perform Polymerase Chain Reaction (PCR). Basic PCR Program...pairs. The initial denaturation step is commonly performed at 94–98°C. Denature 30 seconds at 94°C: Continued...reagent by the number of individual PCRs you wish to perform and add 10% extra to account for pipetting error...

  16. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...standard FPs. The Looger Lab was thus able to easily perform a four-color labeling experiment using smFPs and...References 1. Viswanathan, Sarada, et al. "High-performance probes for light and electron microscopy." Nature...

  18. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ...documents into RSpace, maintaining the original formatting. In this way data is moved from a static and ...conversations and import them into RSpace. The kind of informal conversations about research that can take place...

  19. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    ... these steps, we discover we need additional information. It is quite possible that the clarifications...hows and whys of our users, we are able to make informed software design choices, resulting in software...

  20. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...target. In addition, when they looked at the transformed E. coli expressing Cas7-11, they found no evidence...system as Class I, Type III-E, and then used this information to identify a large variety of related bacteria...
Showing: 681 - 700 of 960 results