We narrowed to 810 results for: mal

1. Hot Plasmids - May 2022

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   ...sequences with reduced risks of unwanted mutations in mammalian cells. Long sequences traditionally have higher...

2. Plasmids 101: Blue-white Screening

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   ... also false negatives: These are rare, but if a small fragment is inserted in-frame, read-through can ...

3. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

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   ... could typically achieve 90-99% knockdown with minimal off-target effects. The system also enabled them...

4. Kiran Musunuru on the Newest TALEN Genome-Editing System

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   ...one can introduce changes into the genomes of mammalian or other cells. TALENs are engineered proteins...

5. The time and cost required to make a plasmid

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   ...to use or build on work done previously, borrow small aliquots of restriction enzymes for one-off projects...

6. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

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   ...further away. While constitutive promoters drove maximal gene expression near genomic origins of replication...

7. Transferable Skills Guide: Problem Solving

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   ...survey found that many researchers feel they lack formal training in a variety of transferable skills. At...

8. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

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   ...pooled library of mutant sensors expressed in a mammalian cell line engineered to be electrically excitable...

9. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

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   ...quantified for individual cells, and the number of 'normal' looking cells can be counted. This assay will ...

10. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

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    ...great gene therapy delivery mechanism, but their small packaging capacity of 4-5 kb limits the delivery...

11. Transferable Skills Guide: Cross-team Communication

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    ...” “material,” “item,” and “format” all received formal definitions in our lexicon. We created documents...

12. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

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    ...expression of ion channels, despite having an otherwise normal genomic background, can even lead to the formation...

13. Summer SciComm Series: Cognitive Load

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    ... faster presentation with more information than normal. I had to learn to observe and adjust on the fly...

14. Antibodies 101: Selecting the Right Antibody

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    ...better resolution as these immune complexes are smaller than a primary:secondaries:conjugates complex. ...

15. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

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    ...should enable researchers to make precise edits in mammalian cells and may decrease worries about off target...

16. Plasmids 101: Golden Gate Cloning

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    ...increased number of fragments, or the ligation of very small/very large fragments, these problems can be overcome...

17. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

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    ...references and sorting out what they mean is no small task. As a scientist, it is useful to make literature...

18. A Look at Addgene's QC Process

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    ...plasmids in bacteria — has many points at which small errors can be introduced. Eventually, these errors...

19. Design Tips for Prime Editing

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    ...2024). Improving prime editing with an endogenous small RNA-binding protein. Nature, 628(8008), 639–647....

20. Plasmids 101: Modular Cloning

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    ...parts delivered) in certain model organisms. In mammals, most CRISPR libraries are assembled and delivered...