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TypeBlog Post...to perform these experiments in the beginning. In most classrooms, the laboratory experience is focused...
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Pathways Over Time Plasmids Engage Students in Functional Genomics Research
TypeBlog Post...the carbon source. Student results have shown that most, but not all, S. pombe Met genes complement the ... -
How to Prepare for an Industry Interview
TypeBlog Post...Interviewing remotely Remote interviews are likely new to most people - even hiring managers and your interviewers... -
Science communication: 9 Strategies to Get Your Foot in the Door
TypeBlog Post...Getting involved in science communication has been the most rewarding step I have taken in my career. The moment... -
Fluorescent Proteins 101: Introduction to FRET
TypeBlog Post... with a gene of interest. These plasmids will be most useful for constructing intermolecular FRET probes... -
Plasmids for Endogenous Gene Tagging in Human Cells
TypeBlog Post...functional in many different human cell types, and while most of the genes we’ve tagged are widely recognized ... -
CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9
TypeBlog Post...transcription start site will terminate transcription of most of the ncRNA (Figure 1D). While this method can ... -
Lab Automation at Addgene
TypeBlog Post...simplistic and it isn’t electronic but it is one of the most helpful machines I have used in the lab. It’s especially... -
Antibodies 101: Choosing the Right Isotype
TypeBlog Post...When probing multiple targets in parallel, use the most narrowly specific secondary antibodies available... -
Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them
TypeBlog Post...store and share, making recombinant antibodies the most practical choice. Hybridomas are much tougher to... -
Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene
TypeBlog Post...will not result in one complete, circular assembly. Most of the time, these plasmids are returned as one ... -
Synthetic promoter AAVs for cell-type specific expression in retinal cells
TypeBlog Post...for finding retinal cell-type specific promoters. Most existing promoters express in a broad number of ... -
Cpf1 Update: Comparison to Cas9 and NgAgo
TypeBlog Post...does not mediate detectable off-target cleavage. Most gRNAs directed low-frequency Cpf1 cleavage at 1-... -
Plasmids 101: FLEx Vectors
TypeBlog Post...Spatio-temporal expression of optogenetic reporters: Since most optogenetic reporters are delivered using AAV injection... -
Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015
TypeBlog Post...the city of Guangzhou and see some of the sites. Most fascinating to me was the outdoor market where one... -
The Materials Science of Optogenetics Experiments
TypeBlog Post... does require a significant amount of equipment, most of which is relatively cheap). Several protocols... -
Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity
TypeBlog Post...NEB (1, 2). While T4 DNA Ligase, the mainstay of most biotechnological cloning efforts for over 50 years... -
Fluorescent Tagging of Endogenous Genes with SapTrap
TypeBlog Post...modified strains that contain your newly tagged gene. Most insertions can only be detected by labor-intensive... -
Hot Plasmids: Summer 2025
TypeBlog Post...Get your sunglasses — these proteins are bright! Most current yellow fluorescent proteins tend to photobleach... -
Magnetic Control of Proteins: More than a Dream
TypeBlog Post...humans (like many organisms) are opaque, which makes most biomedical applications of these tools difficult...