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  3. Management for Scientists: Managing vs. Leading

    Type
    Blog Post
    ...or leading. All three roles are important to accomplishing any goal or finishing any project. Doers are...be successful in a specific role or cycle. For example, if you try to lead when you are expected to be...

  5. Advice for Choosing a Research Project

    Type
    Blog Post
    ...typical research project can take several years to complete and has multiple benchmarks. Think about what ...Researchers should  pick a topic that “looks at the complexity of biology and break apart a piece that can address...

  6. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    ...gene of interest is cloned into/inactivates or completely removes (in the case of Gateway cloning) one ...Posts Learn How to Use Blue White Screening to Simplify Your Cloning Experiments See Where You Can Find...

  7. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    ...cannot be indirectly probed simultaneously on the sample. What to do?  If you’ve ever found yourself facing...visualize both targets in parallel (Fig. 2). For example, if both of your antibodies are rat IgG1, then ...

  8. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    ...able to mediate homology-directed repair when a template is supplied. Find Plasmids from the NgAgo Publication...(so called “gDNAs”) rather than the RNA guides employed by Cas9 or Cpf1. These short, 24 base DNA guides...

  9. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ..., this cell free system allows for a relatively simple, controlled “one-pot” reaction capable of reproducing...found in the metagenome datasets from hypersaline samples, were engineered and adapted for RNAi experiments...

  11. Meet the New Addgene Board Members!

    Type
    Blog Post
    ...Pharmaceutical in 2017. As one of the original employees at Dimension Therapeutics, she helped build and...manufacturing and quality, especially with regard to compliance with health authority regulations,” Baradaran...

  13. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    ...educational materials to our repository of open samples. We are, and have always been, a community resource....43322 Bandrowski, AE, Martone, ME (2016) RRIDs: A Simple Step toward Improving Reproducibility through Rigor...

  15. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    ...from a month to a year. The researcher remains employed by their home institute, meaning the host university...and a priority list of experiments you hope to complete. Share this plan with your host prior to arrival...

  16. Hot Plasmids - January 2023

    Type
    Blog Post
    ...JA. Precise transcript targeting by CRISPR-Csm complexes. Nat Biotechnol. 2023 Jan 23. doi: 10.1038/s41587...antibodies allows you to use them in parallel to help amplify your signal - giving you the benefit of a polyclonal...
Showing: 681 - 700 of 929 results