Addgene

We narrowed to 758 results for: ncl

Showing: 681 - 700 of 758 results

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Plasmids 101: Plasmid Incompatibility

Type

Blog Post

...Asymmetric plasmid loss occurs for several reasons, including one plasmid blocking replication of another and...
Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

Type

Blog Post

... from many with experience mentoring in STEM, including Addgene’s Joanne Kamens. Expanding Binning Singletons...
Transferable Skills Guide: Creativity

Type

Blog Post

...dissenting opinions is to foster a culture of inclusivity. Work with and value people who think differently...
Plasmids 101: Stringent Regulation of Replication

Type

Blog Post

...process. When enough copies of ATP-DnaA are bound, including at the low-affinity sites, the proteins work cooperatively...
Plasmids 101: Broad Host Range Plasmids

Type

Blog Post

...host ranges. Popular replicons from these groups include: RK2 (IncP), RSF1010 (IncQ), and pSa (IncW) (Schmidhauser...
Hot Plasmids - November 2023

Type

Blog Post

...about the whole-brain reference atlas efforts, including a list of validated antibodies, on their webpage...
Twenty Years of Sharing: Expanding Accessibility

Type

Blog Post

...systemic changes, like using alt-text, captions, and inclusive design in our digital content. We have implemented...
Rett Syndrome: A History of Research and Therapeutic Outlooks

Type

Blog Post

...can also find links to cell line repositories including patient-derived iPSCs, MECP2 animal models, tissue...
Neuronal labeling with Spaghetti Monster

Type

Blog Post

...smFPs can result in better resolution images. Conclusion By combining advantages of FPs and epitope tags...
Getting the Most from Your Lentiviral Transduction

Type

Blog Post

...mycoplasma contamination can be subtle and may include reduced saturation density, decreased rates of ...
Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

Type

Blog Post

...dominant-negative and/or toxic. A potential solution is to include an in-frame degron, or degradation sequence, upstream...
Quick Guide to Near-Infrared Fluorescent Proteins

Type

Blog Post

...developed based on miRFP760-miRFP720 FRET pair, including Rac1 biosensor (Shcherbakova et al., 2018) and...
Hot Plasmids: Summer 2024

Type

Blog Post

...variants deposited with Addgene. These variants, including a “miniCRISPR” version, are much smaller proteins...
New Optogenetic Tools for Cytoskeleton and Membrane Control

Type

Blog Post

...attractive strategy for engineering other enzymes. Conclusion We hope this post has illuminated some of the...
Antibodies 101: Normalization and Loading Controls for Western Blots

Type

Blog Post

...axis. This effect causes misleading qualitative conclusions, and it’s also problematic for computational ...
Site Directed Mutagenesis by PCR

Type

Blog Post

... sequencing functional regions of the plasmid including the insert. This is essential since mutations ...
Evolution of Lab Techniques

Type

Blog Post

...high-quality DNA, real-time PCR has several advantages including easier automation, higher throughput screening...
Treating Muscular Dystrophy with CRISPR Gene Editing

Type

Blog Post

... genetic diseases caused by splicing defects, including ataxia telangiectasia, congenital disorder of ...
Technologies Enabled by NanoLuc® Luciferase

Type

Blog Post

...of applications of the Enhanced Nano-Lanterns including monitoring multiple cellular events such as the...
Technique: Probe Phage Genomes for Host Binding Proteins

Type

Blog Post

...refer to the paper for more details on the assay, including examples of RBPs successfully identified using...

Showing: 681 - 700 of 758 results

Results per page:

Plasmids 101: Plasmid Incompatibility

Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

Transferable Skills Guide: Creativity

Plasmids 101: Stringent Regulation of Replication

Plasmids 101: Broad Host Range Plasmids

Hot Plasmids - November 2023

Twenty Years of Sharing: Expanding Accessibility

Rett Syndrome: A History of Research and Therapeutic Outlooks

Neuronal labeling with Spaghetti Monster

Getting the Most from Your Lentiviral Transduction

Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

Quick Guide to Near-Infrared Fluorescent Proteins

Hot Plasmids: Summer 2024

New Optogenetic Tools for Cytoskeleton and Membrane Control

Antibodies 101: Normalization and Loading Controls for Western Blots

Site Directed Mutagenesis by PCR

Evolution of Lab Techniques

Treating Muscular Dystrophy with CRISPR Gene Editing

Technologies Enabled by NanoLuc® Luciferase

Technique: Probe Phage Genomes for Host Binding Proteins

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