We narrowed to 932 results for: omp
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TypeBlog Post...neuroscience is becoming more and more versatile. To complement the wealth of genetically encoded tools available...only reagents, but also method information. To complement these efforts, we’re beginning to share data ...
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Mapping the 4D nucleome with CRISPR/Cas9
TypeBlog Post...with the previously established physical map. In comparing pairs of targets ~2 Mbp apart, they noticed that...that they could evaluate the degree of chromatin compaction even for this small distance! To the authors’... -
6 Steps to Submitting a Resume That Gets Seen
TypeBlog Post... next steps and follow up especially in large companies. It is fine to address the cover letter “To Whom...If the organization is small, any person at the company might be able to help you. If the organization ... -
9 Tips to Achieve Success in Academia
TypeBlog Post.... “Initial and sustained motivation is a huge component of success.” 2. “Enjoy Teamwork.” Be friendly...friendly, open, sharing, rather than intensely competitive. Be curious and interdisciplinary. 3. “There are... -
Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening
TypeBlog Post...lentiviral CRISPR library systems described in companion papers in Science in December were developed as...colleagues, including David Sabatini and Eric Lander, comprise 73,171 guide RNAs (gRNAs) covering a total of ... -
Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes
TypeBlog Post...first collapse arg2 and then arg1 vesicles. By comparing ultrasound images pre-collapse and post-collapse...mouse colon, imaged via ultrasound, and the images compared to mice injected with ECN expressing lux. arg1... -
Some Like it Hot: Thermostable GeoCas9
TypeBlog Post...optimal growth temperature of 55 °C. For a brief comparison of GeoCas9 vs. SpCas9, see the table below and...Yes Longevity in Serum?* No Yes Table 1. Comparing SpCas9 and GeoCas9. *For in vitro Cas9 RNP activity... -
Cellosaurus: A Cell Line Information Database
TypeBlog Post...an ‘-omics’ study or is resistant to specific compounds, links to publication references, and cross-references... publications cited. If the information seems incomplete or unclear, Bairoch follow up via email to the... -
Oh, The Places You Can Go: Careers in Science Communication - Science Writing
TypeBlog Post...], so making it a success feels like a real accomplishment.” He started working on them shortly after ...Development Manager at eScience Labs, an educational company that creates hands-on science lab kits and digital... -
Running for Rare Disease, Running for FOP, Running for AJ
TypeBlog Post...started running on a treadmill. After being with the company for about six months, I learned there was a Genzyme-based...visit us and watch fellow Run4Rare team members complete the Boston Marathon, he wanted to run and play... -
Management for Scientists: Managing vs. Leading
TypeBlog Post...or leading. All three roles are important to accomplishing any goal or finishing any project. Doers are...Leadership Insights for Engineers, Scientists and Computer Professionals as Leaders, by Ken Graham, Ph.D.... -
10 Ways to Share Your Science!
TypeBlog Post...training from an organization such as COMPASS. Although COMPASS has a special interest in ocean science... -
Advice for Choosing a Research Project
TypeBlog Post...typical research project can take several years to complete and has multiple benchmarks. Think about what ...Researchers should pick a topic that “looks at the complexity of biology and break apart a piece that can address... -
History of CRISPR Cas - A tale of survival and evolution
TypeBlog Post...protospacer adjacent motif (PAM). Once the CRISPR/Cas complex binds to the foreign DNA, a cut is made to destroy...eight evolutionarily conserved CRISPR subtypes comprised of more than 40 cas gene families have been characterized... -
Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools
TypeBlog Post...photobleaching, and achieve better signal in vivo as compared to imaging with broccoli aptamers or MS2 tags ...APOBEC3A-Cas9n-UGI and APOBEC3B-Cas9n-UGI base editing complexes are more efficient than the BE3 plasmid containing... -
Single-cell tracking of lineage and identity with CellTag
TypeBlog Post...CellTagging. By constructing lineage trees and comparing them with the gene expression changes of more ...CellTags for tagging two cell populations in a competitive transplant assay in the mouse intestine, demonstrating... -
Safe Port in a Storm...How Addgene is Weathering the Pandemic
TypeBlog Post...here and now. Our closure was earlier than most companies. Since the start of this crisis, we’ve been very... in our building that someone from a different company had tested positive for COVID-19 (it turned out... -
Viral Vectors 101: Parts of the AAV Transfer Plasmid
TypeBlog Post...an ITR during routine plasmid propagation, with complete ITR loss abolishing AAV packaging. To prevent ...secondary structure and high GC content, but some companies now offer Sanger sequencing conditions which can... -
Science Careers: Unruly Interests Feed Many Paths
TypeBlog Post...individual diversity to get from gamete to a visibly complex - and unique - adult. My initial research directions...evolutionary perspective brought by studies of comparative anatomy showed me that some of the most fascinating... -
Addgene Begins Distribution of Recombinant Antibodies
TypeBlog Post...given antibody is available through different companies (Bandrowski et al., 2016). It’s also been well...creating an array of educational materials to accompany our antibody collection. Addgene is sharing our...