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We narrowed to 996 results for: ran

Showing: 681 - 700 of 996 results

  1. Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation

    Type
    Blog Post
    ... synthesis companies often work within a narrow range for GC content. Rare codons also are not good predictors...sequences is Proteobacteria - the most frequent transfer of genes is from Proteobacteria to mammalian expression...natural gene sequence is, the less likely it is transferred into another organism in the lab. Predicting ...

  2. Plasmids 101: Origin of Replication

    Type
    Blog Post
    ...plasmids from the same group should not be co-transformed, so if you need two plasmids for an experiment... same incompatibility group should not be co-transformed. +Actual copy number varies. See below for ...stocks, agar stabs, or liquid cultures. Freshly transformed cells also give a higher plasmid yield that colonies...

  3. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    ...sequences required for viral replication and transcription as well as reporter genes. To learn how you ... your research, check out this post! R Bodies: Membrane-Rupturing Microscopic Tools In this post, Jessica...also showed that R-bodies can rupture E. coli membranes. Read the full post for potential applications...

  4. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ..., K. phaffii is exceptionally good for post-translational modification of recombinant protein products...proteins. Peroxisome studies Peroxisomes are small, membrane-bound organelles essential for sequestering certain...non-homologous end joining (NHEJ) to perform DNA double strand break repair instead of homologous recombination...

  6. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    ...Resistance gene and Selection Markers) and one “Transfer Plasmid” game board. Once they agreed on each ...had 35 minutes to develop a strategy and build a transfer plasmid that could be used to genetically manipulate... this way, the students also had to practice transferable skills like team building, collaborative critical...

  7. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    ...particular study, which includes comparing the transduction patterns of different AAV serotypes. To help... Biosensor AAV The ability to detect the neurotransmitters and other bioactive molecules is integral ...ppAAV-CKIIa-stGtACR1-FusionRed New tetracycline transactivators Plasmid Serotype Name 99120  AAV1 pAAV-ihSyn1...

  8. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    ... The space was modern with clean bold colors of orange and white. The decor was very contemporary and ...components. Without proper processing, these small DNA strands can form concatemers in later steps. This new NEB...hours. It was robust, capable of generating a wide range of insert sizes and also produced high yields of...

  9. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    ...ready-to-use lentivirus from Addgene, you’ll also receive transfer plasmid DNA that you can use along with lentiviral...Addgene Blog Get the Most from Your Lentiviral Transdution Using Virus in Your Research: A Primer for Beginners...Beginners 5 Tips for Troubleshooting Viral Transductions Additional Resources on Addgene.org Visit the...

  10. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    ...used to stay up past my bedtime, poring over my grandparents’ medical textbooks by torchlight under the covers... “motivated reasoning,” the catchall term for a range of neat little tricks our minds perform to avoid...difference between airborne and aerosolised droplet transmission and how different fabrics and mask designs could...

  11. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type
    Blog Post
    ... Figure 2: (A) a smeary dot plot showing a range of antibody readouts. (B) A gate on the smeary dot...histogram plot for a smeary antibody readout. The orange peis the baseline expression of the protein of ...yes-no histogram plot shown previously. Here, the orange peak is a baseline expression of a protein while...

  12. Antibodies 101: ChIP

    Type
    Blog Post
    ...Fragmenting  The next step is break the DNA into random, small pieces of roughly equal size, typically ...enzymatic digestion. Sonication provides a truly random digestion, but is harsher and requires more optimization...your protein of interest is a low abundance transcription factor or cofactor. In such cases, the gentler...

  13. Antibodies 101: Isotypes

    Type
    Blog Post
    ...that helps attack extracellular pathogens.  Transportation of antibodies to places they can’t reach on...region, can be bound by special receptors that transport antibodies through cells and into different body...tears, saliva, mucus) where it protects mucosal membranes. This isotype is most commonly a dimer with a ...

  14. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    ...expression, researchers often need to determine these transduction patterns empirically for their own experiments...AAV2 pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP Brandon Harvey 50457 AAV2 pAAV-hSyn-DIO-EGFP Bryan Roth...system takes advantage of the extensive supply of transgenic reporter lines that supply a variety of GFP expression...

  16. How to Make Friends and Meet People at a Scientific Conference

    Type
    Blog Post
    ...these people you meet will review your papers or grants in the future, and don’t be surprised to know that...personally Scientists in your field will know about grants and projects that might help your work Tenure can...just a meeting, but having lunch twice turns a stranger into a friendly colleague. Recommended Reading...

  17. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    ...and animals. Unlike bacteria, yeast can post-translationally modify proteins yet they still share many of... genetic system, and a highly versatile DNA transformation system.Unlike most other microorganisms, yeast...plasmids oftentimes utilize an ARS to aid in high transformation efficiency; however, this region does not necessarily...

  18. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type
    Blog Post
    ...meeting at both the Fox Chase Cancer Center and Brandeis University. 5 easy steps to forming your own peer... of people who are all interested in making a transition. Schedule your first meeting and get organized... your successes and support each other as you transition to the next stage in your careers! Stay in touch...

  19. Addgene: The First Twenty Years

    Type
    Blog Post
    ...Part of that work meant facilitating the Material Transfer Agreement process, which was a major barrier for... for depositors and requestors alike. We transformed this process by aligning institutions with standardized...Addgene. I think resources like this are why my brand of neuroscience has exploded. Because we have a ...

  20. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...the embryos underwent repair not from the single-stranded oligonucleotide (ssODN) template supplied by Ma...maternal allele rather than the supplied single-stranded oligonucleotide (ssODN) template. Embryos also...off-target effects, but this advantage likely won’t transfer to all genes we’d like to target. In addition,...
Showing: 681 - 700 of 996 results