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We narrowed to 992 results for: ran

Showing: 681 - 700 of 992 results

  1. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    ...particular study, which includes comparing the transduction patterns of different AAV serotypes. To help... Biosensor AAV The ability to detect the neurotransmitters and other bioactive molecules is integral ...ppAAV-CKIIa-stGtACR1-FusionRed New tetracycline transactivators Plasmid Serotype Name 99120  AAV1 pAAV-ihSyn1...

  2. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    ...used to stay up past my bedtime, poring over my grandparents’ medical textbooks by torchlight under the covers... “motivated reasoning,” the catchall term for a range of neat little tricks our minds perform to avoid...difference between airborne and aerosolised droplet transmission and how different fabrics and mask designs could...

  3. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ..., K. phaffii is exceptionally good for post-translational modification of recombinant protein products...proteins. Peroxisome studies Peroxisomes are small, membrane-bound organelles essential for sequestering certain...non-homologous end joining (NHEJ) to perform DNA double strand break repair instead of homologous recombination...

  4. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    ...ready-to-use lentivirus from Addgene, you’ll also receive transfer plasmid DNA that you can use along with lentiviral...Addgene Blog Get the Most from Your Lentiviral Transdution Using Virus in Your Research: A Primer for Beginners...Beginners 5 Tips for Troubleshooting Viral Transductions Additional Resources on Addgene.org Visit the...

  5. Antibodies 101: Isotypes

    Type
    Blog Post
    ...that helps attack extracellular pathogens.  Transportation of antibodies to places they can’t reach on...region, can be bound by special receptors that transport antibodies through cells and into different body...tears, saliva, mucus) where it protects mucosal membranes. This isotype is most commonly a dimer with a ...

  6. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    ...expression, researchers often need to determine these transduction patterns empirically for their own experiments...AAV2 pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP Brandon Harvey 50457 AAV2 pAAV-hSyn-DIO-EGFP Bryan Roth...system takes advantage of the extensive supply of transgenic reporter lines that supply a variety of GFP expression...

  8. Antibodies 101: ChIP

    Type
    Blog Post
    ...Fragmenting  The next step is break the DNA into random, small pieces of roughly equal size, typically ...enzymatic digestion. Sonication provides a truly random digestion, but is harsher and requires more optimization...your protein of interest is a low abundance transcription factor or cofactor. In such cases, the gentler...

  9. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type
    Blog Post
    ... Figure 2: (A) a smeary dot plot showing a range of antibody readouts. (B) A gate on the smeary dot...histogram plot for a smeary antibody readout. The orange peis the baseline expression of the protein of ...yes-no histogram plot shown previously. Here, the orange peak is a baseline expression of a protein while...

  10. How to Make Friends and Meet People at a Scientific Conference

    Type
    Blog Post
    ...these people you meet will review your papers or grants in the future, and don’t be surprised to know that...personally Scientists in your field will know about grants and projects that might help your work Tenure can...just a meeting, but having lunch twice turns a stranger into a friendly colleague. Recommended Reading...

  11. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    ...and animals. Unlike bacteria, yeast can post-translationally modify proteins yet they still share many of... genetic system, and a highly versatile DNA transformation system.Unlike most other microorganisms, yeast...plasmids oftentimes utilize an ARS to aid in high transformation efficiency; however, this region does not necessarily...

  12. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type
    Blog Post
    ...meeting at both the Fox Chase Cancer Center and Brandeis University. 5 easy steps to forming your own peer... of people who are all interested in making a transition. Schedule your first meeting and get organized... your successes and support each other as you transition to the next stage in your careers! Stay in touch...

  14. dTAG - You're it!

    Type
    Blog Post
    ...example, IZKF1 and IZKF3 are cell-type specific transcription factors with important roles in Multiple Myeloma... (Nabet et al., Erb et al., Huang et al.), transcriptional and epigenomic profiling studies (Nabet et ...Featured molecules: dTAG-7 and dTAG-13 Erb et al. Transcription control by the ENL YEATS domain in acute leukemia...

  15. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    ... 3’ end. This repeat region associates with the trans-activating crRNA (tracrRNA) which interacts with... important cellular RNA molecules with post translational modifications. One of the most common of these...targeting experiments allows you to visualize transfection efficiency and even track localization in cellular...

  16. Click Biology: How to Click and Play to Explore Biology

    Type
    Blog Post
    ...of the coupling system include: Wide fusion-tolerance — Click Biology tags should be similarly useful...parts in the micromolar or nanomolar concentration range. Reactions to happen under lots of conditions ...the Brainbow system, adds Tetracycline (Tet) transactivator to increase fluorescent protein expression ...

  17. Addgene: The First Twenty Years

    Type
    Blog Post
    ...Part of that work meant facilitating the Material Transfer Agreement process, which was a major barrier for... for depositors and requestors alike. We transformed this process by aligning institutions with standardized...Addgene. I think resources like this are why my brand of neuroscience has exploded. Because we have a ...

  18. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...the embryos underwent repair not from the single-stranded oligonucleotide (ssODN) template supplied by Ma...maternal allele rather than the supplied single-stranded oligonucleotide (ssODN) template. Embryos also...off-target effects, but this advantage likely won’t transfer to all genes we’d like to target. In addition,...

  20. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    ...poison, locking up DNA gyrase with broken double stranded DNA and ultimately causing cell death. ccdA, another...gene in frame with an MCS. E. coli that were transformed with the empty vectors expressed the ccdB gene... that ccdB Survival™ can be more difficult to transform, depending upon the specific plasmids being used...
Showing: 681 - 700 of 992 results