We narrowed to 969 results for: ARC

...was contributed by Marco Straccia, an Associate Professor at University of Barcelona. The challenge: Making...fre-sci.info Marco Straccia is currently an Associate Professor at University of Barcelona. He is also ..., I and other professors at the University of Barcelona wanted to develop strategies to make our classes...

...guest blogger Chris Richardson, a Postdoctoral Researcher in Jacob Corn’s lab. CRISPR-Cas9 (Cas9) is an...strategies to manipulate that process. We began our research with a simple question: how do Cas9 and sgRNA ...donor DNA. Chris Richardson is a Postdoctoral Researcher in Jacob Corn’s lab investigating DNA repair ...delivery of purified Cas9 ribonucleoproteins." Genome research 24.6 (2014): 1012-1019. PubMed PMID: 24696461....Our CRISPR Guide Find CRISPR Plasmids for Your Research Check out Our List of gRNA Design Tools ...

...requirements. These different PAM requirements allow researchers to target many different genomic loci. 3. Cleave.... When using CRISPR/Cas9 for genome editing, researchers simply need to express a gRNA designed to direct...elements separated by unique sequences. When researchers first discovered these arrays, they did not know...them “spacers” (see figure below). After years of research, we now know that each direct repeat, combined...leaving a F) double-strand break. A “gRNA” is a researcher-designed hybrid of the tracrRNA and the crRNA...

...story of base transversion editors starts with researchers in the Keith Joung lab, who were engineering ...editing between bacteria and mammalian cells Researchers in the Xueli Zhang lab were making similar observations...develop more efficient and precise editors. Most researchers agreed on the first part of the likely editing... efficacy varied among target sequences. The researchers hypothesized that the CGBE edit process occurs...experimented with adding different proteins to the CGBE architecture. They conducted a CRISPRi screen to identify....1038/nbt.4172 Kurt, I. C., Zhou, R., Iyer, S., Garcia, S. P., Miller, B. R., Langner, L. M., Grünewald...

...continue to accelerate research and discovery by improving access to useful research materials and information...have been lost to continue to be available to the research community.    Validation For most cloning projects...construct by Sanger sequence analysis. But for this research, it was likely that the sequence of the functional...range secondary antibodies are commonly used in research laboratories to detect the different subclasses...

... Neural Dynamics and HHMI’s Janelia Research Campus. His research focuses on developing biosensors and...GECIs) have become a vital tool in neuroscience research. These fluorescent proteins light up in response...sensors in multicolor experiments. With OCaMP, researchers can image multiple brain regions or cell types...medical student at the University of Calgary and a research associate with Alexander Lohman at the Hotchkiss...

...iPS cells and embryos make a case for conducting research in embryos should our society decide that germline...not being used to establish a pregnancy, the researchers were able to examine each individual blastomere...embryos and iPS models, it appears that embryo research is the only way to answer questions of efficiency...Given moral and ethical concerns surrounding such research, it is crucial that scientists proceed with caution...non-biologists alike. Given the public significance of this research, we have the obligation to discuss it as openly...

..., expanding its practicality and popularity in research (Tanenbaum et al., 2014, Konermann et al., 2015...the most popular methods for CRISPR activation, research in the area has continued to develop. These newer...an engineered CRISPR-Cas system. Nucleic Acids Research 41:7429–7437 . https://doi.org/10.1093/nar/gkt520...Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H,...Addgene's CRISPR Guide Find CRISPR plasmids for your research ...

...Find viral preps for your research Find chemogenetics plasmids for your research ...Jendryka et al., 2019), they have resulted in a search for alternative ligands, such as C21, CLZ, Perlapine.../10.1021/cn500325v Goutaudier, R., Coizet, V., Carcenac, C., & Carnicella, S. (2019). DREADDs: The Power...Sternson S (2019) Ultrapotent chemogenetics for research and potential clinical applications. Science 6436...

...again reminded of the extent that C. elegans researchers embrace open science and share resources and ... what’s new and popular! References Hammarlund, Marc, et al. "The CeNGEN Project: The Complete Gene Expression...developmental dynamics database." Nucleic acids research 41.D1 (2012): D732-D737. PubMed PMID: 23172286...23172286. PubMed Central PMCID: PMC3531189. Mangone, Marco, et al. "UTRome. org: a platform for 3′ UTR biology...biology in C. elegans." Nucleic acids research 36.suppl_1 (2007): D57-D62. PubMed PMID: 17986455. PubMed ...