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We narrowed to 955 results for: ARC;

Showing: 351 - 360 of 955 results

  1. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type
    Blog Post
    ...adapted CRISPR nucleases for many more specific research needs. In this update, we will focus on the most... variants may be appropriate for your specific research question. Considerations when choosing your Cas...recognize a variety of different PAM sequences. Researchers have also used experimental evolution to develop...that may make it especially suitable for in vivo research and potentially treatments. Cas12b, characterized...al., 2018) Labs also shows promise for in vivo research due to its small size and specificity relative..., it is likely that the toolkit available to researchers will continue to expand. Final thoughts Since...

  2. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    ...provide a much needed boost to basic molecular plant research, more initiatives are needed to increase the body...Organizations like Open Plant can then build on this research and effectively carry out their mission of creating...selected a project in a field with 30 years of basic research standing behind it. Our color changing flower ... Petunias have been a model organism for plant research for decades, and the biochemical pathways involved...circadian rhythm in petunias. Because of the extensive research that has come before us, it’s possible for us ... flowers that change color. Without this basic research – this comprehensive understanding of the genetic...

  3. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    ...environments has never been easy and, for many years, researchers lacked good tools to directly follow immune cells...thymus to the lymph nodes. These methods gave researchers much more information regarding immune cell mobility...coloured lymphoid organs did in the past. How do researchers track fluorescently labeled cells? 1. Epifluorescence...For the first time, fluorescent proteins gave researchers the ability to track immune cells within organs... the next revolution in cell imaging, giving researchers the ability to monitor cellular movements in ...cells is the the Cre/lox recombination system. A researcher can flank fluorescent proteins in a plasmid with... PubMed Central PMCID: PMC3164316. 5. Chieppa, Marcello, et al. "Dynamic imaging of dendritic cell extension...

  4. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

    Type
    Blog Post
    ...technology that’s already had a huge impact on research and will be affecting our everyday lives very ...visualizing genomic loci, and much more. Now, researchers from the Zhang, Joung, and Doudna labs have improved...effects, respectively. Recognizing these issues, researchers from the Zhang lab at the Broad Institute, the...structure leads to the development of HypaCas9 Researchers in the Doudna Lab at Berkeley recently added ...eSpCas9, SpCas9-HF1, and HypaCas9 should enable researchers to make precise edits in mammalian cells and ...question very soon! We look forward to seeing how the research community makes use of these tools and are excited...

  5. Rabies and Neuronal Tracing

    Type
    Blog Post
    ... of us learned in biology is that the body’s architecture and plumbing are important. We started with ...oxygenates the body. However, in neuroscience, this architecture is frequently still an open question. The connections...recombinant viruses typically used in neuroscience research (e.g., AAV) are useful for labeling the individual...recombinant forms of these viruses typically used in research aren’t able to replicate, and for a virus to cross...receptor (TVA) in user-defined cells. For example, a researcher can deliver Cre-dependent TVA to Cre-expressing... (i.e., it is taken up by projection neurons). Research into these phenomenon has shown that the RABV ...

  6. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ...team effort and many late nights, not unlike most research, but the urgent need for the test did catalyze...yet never hesitated to reach out to the greater research community when we needed additional expertise....uses portions of the pathogen’s genome as unique barcodes to identify samples containing the virus. Of the...rapid sharing of these tools with the Zika virus research community. Find the Zika Sensor Constructs at ... of Pharmacy at the University of Toronto. His research is focused on developing cell-free synthetic biology...Arizona State University. His group carries out research in synthetic biology, nanomaterials science, and...

  7. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...molecular biology in our Plasmids 101 eBook! Researchers use various techniques to control protein expression..., biotechnological, and medical applications. Researches can visualize proteins in vivo by tagging them... also be purified for use in molecular biology research (eg. polymerases and other enzymes might be purified...types of plants such as maize, tobacco, rice, sugarcane, and even tubers of potatoes have been used for...and biochemistry. When using S. cerevisiae, researchers often place recombinant proteins under the control...Resources on Addgene.org Bacterial expression systems Search for plasmids by expression system ...

  8. PhD Applications After COVID

    Type
    Blog Post
    ...Principle Investigators (PIs) - whose labs does research that aligns with your interests and goals.  Reaching...gives a more comprehensive idea of that advisor’s research interests which can be helpful in creating your...where you can further discuss aspects of the lab, research, and provide them with a CV or transcripts if ...interests, but will ask you questions about your research goals and education, too! Prepping for the interview...quickly reference your questions about the PI’s research or the name of the award you won for your conference... student at George Washington University whose research focuses on Drosophila-parasite pathogen interactions...

  9. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

    Type
    Blog Post
    ... more than 10 modules often rely on two-step hierarchical approaches using different Type IIS restriction...Development of Golden Gate Assembly test systems Our research focused on three different levels of assembly:...number of transformants. To compensate for this, researchers can plate larger volumes of the outgrowth containing... to minimize this possibility. Recently, NEB researchers profiled the comprehensive fidelity of cohesive...important tools for many areas of science, and researchers continue to test the limits of DNA assembly approaches...and bias during DNA end-joining." Nucleic acids research (2018). PubMed PMID: 29741723. PubMed Central ...

  10. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    ...and professional development opportunities for researchers interested in connecting with the public. My ...viewing my activities as types of experiments. I searched for new opportunities with an open mind and open..., funny, picture-filled blog posts about their research? Then we could get everyone to share their articles...This seemed like a great way to share some of the research taking place at our university with the community...resource that has gained a lot of traction with researchers. It is an excellent way to meet people with similar...Resources on the Addgene.org Find Plasmids for Your Research Learn About A Variety of Different Fields on Our...
Showing: 351 - 360 of 955 results