We narrowed to 653 results for: EPO
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TypeCollection...activity reporter of SARS-CoV-2 main protease Mpro. (Unpublished) A FlipGFP-based activity reporter of SARS-CoV... also lists several luciferase and fluorescent reporter plasmids that have been used for measuring viral...method termed Specific High Sensitivity Enzymatic Reporter UnLOCKING (SHERLOCK and SHERLOCKv2). The Broad... termed DNA Endonuclease Targeted CRISPR Trans Reporter (DETECTR). Mammoth Biosciences has published information...(Link opens in a new window) CRISPR tools and reporters now available from Stanley Qi's lab. COVID-19 ...previously we will reach out to the lab to ask them to deposit the plasmid with us. Return to top Pooled Libraries...
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Microbiology Resources
TypeCollection... aren't in the repository? Help us widen our resources for the community by depositing your useful tools...Microbiology Plasmids and Resources Addgene’s repository contains plasmid tools for a range of microbial...bacteria, and more. Synthetic Biology : Browse our deposited tools and resources, or read our guide on assembly... reference page for CRISPR resources, such as depositor protocols and gRNA design software. Addgene yeast...Genome Engineering blog post to see how Addgene depositors are making this possible in a range of organisms... -
CRISPR Plasmids - Tagging
TypeCollection...The Eric Mendenhall and Richard Myers labs have deposited plasmids for a CRISPR-based system to add a tag...homology arms and selection marker. The first deposited plasmids in this CRISPR-Cas tagging system were... Tagging System Recently, Yannick Doyon's lab deposited a plasmid which introduces an N- or C- terminal...PITCh Tagging System The Takashi Yamamoto lab has deposited the PITCh system plasmids for CRISPR-based knock-in...microhomologous sequences and gRNA target sites. The first deposited PITCh plasmids were tested by fusing EGFP-2A-PuroR...with the following article: Natsume, et al. Cell Reports 2016 Förstemann Drosophila Cell Tagging System ... -
Bikard Lab - CRISPR Repression Collection
TypeCollection... Depositor Collections Bikard Lab - CRISPR Repression Collection...David Bikard Lab at the Institute Pasteur has deposited a collection of CRISPR arrays to modulate the ...whole range of expression levels for these two reporters simultaneously. By fusing sfGFP and/or mCherry...cross‐talk. (A) Schematic of the strain expressing two reporters and dCas9 under a P-tet promoter integrated in...phage attachment sites. The levels of the two reporters, mCherry and sfgfp in this case, can be controlled... -
Zebrafish Plasmid Collection
TypeCollection...editing, empty backbones, fluorescent proteins, reporters, biosensors, and Cre expression plasmids. Large...studying diseases, are listed below: Lipid droplet reporters - Richard White Lab. An imaging platform to study...using red light. LipoGlo - Steven Farber Lab. A reporter system that allows direct assessment of lipoprotein...International Resource Center (ZIRC) - A US-based repository for zebrafish strains and materials and information...European Zebrafish Resource Center (EZRC) - A repository at the Karlsruhe Institute of Technology (KIT... -
CRISPR Guide
TypeCollection...and a ssDNA-fluorescently quenched reporter is added. As this reporter is degraded by Cas12a, it will release... using a fluorescent reporter. In SHERLOCK, a quenched ssRNA fluorescent reporter is added to the reaction...following the amplification protocol specified by the depositing lab. After amplification, you’ll need to use ... target sequence, it promiscuously cleaves the reporter as well, and a quantifiable signal is produced...CRISPR 101 eBook (3rd edition) CRISPR software and depositor protocols JOVE video reprint: Protocol for genomic...NNNNGATT Streptococcus thermophilus (ST) 3' NNAGAAW Treponema denticola (TD) 3' NAAAAC Additional Cas9s from...all-in-one CRISPR/Cas9 vector system. Scientific Reports , 4 (1). PMID: 24954249 Shechner, D. M., Hacisuleyman... -
Cell Migration Consortium Plasmids
TypeCollection... Addgene, which highlights CMC depositing labs and plasmids.... Depositor Collections Cell Migration Consortium...laboratories of the Cell Migration Consortium have deposited plasmids useful for cell migration research at...at Addgene. CMC investigators who have deposited plasmids include: Joan Brugge , Harvard Medical School... -
Plan Your Experiment
TypeCollection...promoter is typically used for gRNA May contain reporter gene (e.g. GFP) to identify and enrich positive...vector or separate transfer vectors May contain reporter gene (e.g. GFP) or selection marker to identify...Additional Resources: CRISPR protocols from Addgene depositors JOVE Video Reprint: Protocol for genomic deletions...in question (see CRISPR protocols from Addgene depositors ). Deliver Cas9 and gRNA Choose a delivery method...facilitates CRISPR/Cas9-based genome editing. Scientific Reports . 5 , 11315. PMID: 26066060 Zuris, J. A., Thompson... -
Brzezinski Lab CRISPR Collection
TypeCollection...with EF1alpha addition of an mCherry reporter variant nuclear reporters via an H2B fusion shuttle plasmid... Depositor Collections Brzezinski Lab CRISPR Collection Brzezinski...advantage of the Zhang lab all-in-one (guide/Cas9/reporter) pX458 plasmid family. They have made several ... -
Distribution to Industry
TypeCollection...Addgene’s deposit agreements allow Addgene to distribute plasmids on behalf of the depositing institutions...legal agreement between the recipient and the depositing institution(s). It governs the transfer of biological...entity is qualified to receive materials from our repository. All purchases are subject to Addgene’s Terms...