We narrowed to 1,073 results for: TOR;

...express in a broad number of cell types, such as inhibitory neurons, rods, cones, or retinal ganglion. Rather...repeating mouse cell type specific transcription factor binding sites interspaced with random sequences...: transcription start site. TFBS: transcription factor binding site. Image from Jüttner et al., 2019 with...types (ex: rods or cones) or cell classes (ex: photoreceptors which encompassed both rods and cones).  Targeted... to target a cell class in mice is not a good predictor for targeting the same cell class in NHPs (R=0.38... to target a cell class in monkeys was a good predictor for targeting the same cell class in humans (R...also expressed. If Cre-mCherry expresses in photoreceptors and horizontal cells, and Cre-dependent-GFP...

... plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles.  Here's what...single-molecule biophysics Fast and responsive voltage indicator ONE-GO biosensors kit Antibodies for neurodegeneration...order to study the conformational dynamics of the motor protein kinesin-1, the Twelvetrees Lab took advantage...2024.12.20.629623.  A fast and responsive voltage indicator with enhanced sensitivity for unitary synaptic...synaptic events By Brian O’Neill The Lin Lab and collaborators recently created ASAP5, a faster and more responsive...responsive genetically-encoded voltage indicator (GEVI) than previous versions. ASAP5 can be imaged using... and subthreshold events, including miniature excitatory postsynaptic potentials (mEPSPs) (Hao and Lee...

...fluorophores can “spill over” into detectors that are not the primary detector for that fluorophore. For example...single (primary) detector to identify a fluorophore, spectral flow cytometers use all detectors for all fluorophores...panels: five laser spectral flow machines have 64 detectors, allowing for the use of up to 64 markers in a...emitted by a fluorophore is guided to a single detector using a series of mirrors and filters. Importantly...Importantly, conventional flow cytometry uses one detector to detect one fluorophore and therefore one cellular... FITC signal is spilling over into the primary detector for PE. In order to correct for this, compensation...the fluorescent molecules can be quite broad, detectors will usually only pick up a small portion of that...

...to their laboratory. A single mouse click on a gene of interest will direct the investigator to a list... cancer are yielding promising results in the laboratory, and Addgene is accelerating this research by...proto-oncogenes and tumor suppressors to the accelerator and brake pedal of a vehicle, respectively. In... (now an oncogene), this is analogous to the accelerator of a vehicle being stuck down. Similarly, a mutation...cells still need to successfully infiltrate the circulatory or lymph systems to spread, or metastasize. Many...Many cancerous cells will die trying, as the circulatory and lymph systems are not ideal environments ...Clone Collection 2.0. The collection is a set of vectors for use with the Gateway Cloning Platform (Life...

...from a GFP variant and a DA receptor such that GFP fluoresces when the receptor is bound by DA. Two variants... variant bound to a DA receptor, such that the GFP fluoresces upon DA-receptor binding (Sun et al., 2018...Find dopamine and other biosensors in our AAV vector inventory. References Darvesh, Altaf S., et al. "In... of our viral vectors blog posts Find tips for preparing AAVs Download our viral vectors 101 eBook Resources...in reinforcement learning, decision making, and motor control.  What do we understand about Dopamine?...ms on and 0.7 or 2.5 s off, for mouse or human receptors, respectively). Using GRABDA sensors in experiments...rat brain, and its similarity to the “dopamine receptor”." Proceedings of the National Academy of Sciences...

...finally decide the LEGO laboratory was good enough. There are no pre-made LEGO laboratory sets so we had to ...to show people the very human struggles in the laboratory routine. We hope that sharing and laughing at...the LEGOs were mirrored by the nearly infinite regulatory capabilities of gene pathways just waiting to...was creating a LEGO stage that resembles a real laboratory. It only took one night to build the first model...use our imaginations to mold it all into a full laboratory. It is priceless to see our “toys” gaining life...working as scientists in a custom made, colorful laboratory. When we started, the hardest part of the process...building each scenario, I go “WOW, now I have my own laboratory” (the main character is much more than a piece...

...Despite its relatively recent development, the history of optogenetics begins almost 50 years ago with...Wick and Krook-Magnuson, 2018). Optogenetic FLEx vectors contain a strong promoter upstream of an opsin ...Cre-dependent viruses, such as the optogenetic FLEx vectors in combination with transgenic animals or rAAVs...example, Taylor and colleagues used rAAV FLEx-based vectors to study the neural circuitry behind the mechanisms...paper, de Jong and colleagues used rAAV FLEx-based vectors to answer this question, mapping and characterizing...spatio-temporal precision and reversible modulation of FLEx vectors combined with the use of several Cre transgenic...motivated behaviors (de Jong et al., 2019). FLEx vectors represent, therefore, an ideal partner to optogenetics...

... learning. 1. Create a peer mentoring group We don’t all get the mentoring support we need, and most of...to access great mentorship is by recruiting your own peers and classmates! Your mentors don’t need to be...Own Peer Mentoring Group: A How-To Guide for Scientists.   Once in-person, peer-mentoring groups ... of us could use more mentors to listen, guide and support us during training and throughout our careers...goals.  I’ve been surprised to see that virtual mentoring groups seem to be as successful as in person groups...? You could do a talk virtually for your peer mentoring group (see above). However, now that we are all...initiative. In fact, hear from Addgene’s former blog editor Tyler Ford on how initiative landed him at Addgene...

...transcriptional regulator to modulate expression at that locus. The use of regulators allows a greater...shifted in recent years toward tinkering with regulatory networks and multiplex gene control. However,...approaches are often met with the challenges of regulatory bottlenecks and their scope is limited by the...Far removed from the bio-industry’s vision of ‘biofactories’, most successes in metabolic engineering have...us one step closer to the vision of metabolic biofactories by demonstrating the use of CRISPR-based RNA...scaffolds that can recruit various transcriptional regulators (Zalatan et al. 2015).  These scaffolds are stretches...levels dependent upon the type of transcriptional regulator or the number of designed scaffold binding sites...

... at some of the stories behind the names. We also have many plasmids in our repository that you can use...to a specific set of DNA-binding transcription factors to repress the expression of genes primarily involved...Cancer Institute! Sonic Hedgehog, an important regulator of embryonic development The largest conference...the hedgehog signalling pathway, which is a key regulator of all bilateral animal development. SHH plays...publication, Ivics et al 1997. Spaghetti Squash, a regulator of cellular contraction You may think that a gene...vessels). Spaghetti squash (Sqh) is the Drosophila Regulatory Light Chain (RMLC) of nonmuscle myosin II (Karess... Jordan and Karess, 1997), which contains the regulatory regions that are necessary for myosin activity...