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...possibilities enabled by this highly modular AAV - that’s probably why it’s so popular! We’re excited to see how ...serotypes AAV1, AAV5, and AAV retrograde (the most popular form), this viral vector is used to deliver DNA...specific Cre can provide you with that control in a modular way. For example, say you would like to label ...Cre-controlled gene of interest makes pmSyn-BFP-Cre highly modular. Further uses of pmSyn1-EBFP-Cre Of course there...there are also more complicated ways to use this particular AAV. For instance, you might use it as part of...

...reinventing the wheel is inefficient. An effective team regularly seeks expertise wherever it can be found. Listen...allies" – these are employees in your group that regularly step up to give feedback and fill you in on percolating...constructive input from your team: Solicit feedback regularly and often A one-time event doesn't work to get...in non-public settings. Ask over coffee.  Have regular 1:1 meetings with team members (don’t become known...experts recommend using anonymous surveys with regular frequency. Anonymity can be a great way to get ...patience, pause; A = ask at least one question to clarify the situation; C = confirm that you have understood...

...scientists want to transfer large genes, smaller binary vectors with a larger cloning capacity would be ... of DNA that will be transferred to the plant, similar to viral vectors’ terminal repeats. It’s a lot ... most first-generation binary vectors are quite large (>10 kb.) Since transformation efficiency is inversely...pKYLX vectors, respectively, making it ideal for large gene transfer. The M24 promoter provides constitutive... the plasmid can also be customized due to its modular structure. Two multiple cloning sites (MCS's) flank...

...cells infected with malaria or other parasites. Addgene: Do you have any particular tips or tricks for ...generating new tools to manipulate biology based on molecular features found in nature, with the ultimate goal...improve the diagnosis of disease, and cancer in particular. They recently introduced the SpyTag/SpyCatcher...What is the advantage of peptide tags for building larger protein assemblies? Howarth: Peptide tags can be...systems and they shouldn’t get in the way much. Particularly we wanted to be able to build assemblies through...

...over the years, he and his colleagues built a Molecular Expressions collection including some 3,300 plasmids...designed in the same vector backbone with very similar restriction enzymes. You can essentially cut and...of many proteins known to localize to specific cellular structures.The Davidson lab even included some...his team created with the research community at large, "[Fluorescent proteins are] a whole new way of ...everywhere to get a clearer picture of that intracellular world, even as the Davidson lab prepares to ...scientists, and the FSU team who have made this large, important plasmid deposit possible.   ...

...Haoyi Wang! Wenning Qin is a molecular biologist with Biogen and is particularly interested in applying CRISPR...the go-to choice for studying mammalian biology. Similar to any model, mice are not without their problems...manipulate the mouse genome using CRISPR/Cas9. Similar to the human genome, the mouse genome is made up...exciting – the cell sends out an “SOS” signal and cellular repair mechanisms rush in to repair the damage...generating small mutations with HDR, when it comes to larger scale genome editing, such as replacement of a ...degradation by the omnipresent RNase. You may want to declare your bench “RNase free” and refrain from talking...

...Transposagen. He has a long-standing interest in molecular and cellular biology and brings 10 years of research...mutations behind. Furthermore, piggyBac™ offers a large cargo-carrying capacity (over 200 kb has been demonstrated...Controlling the expression levels of your gene may be particularly useful when the gene being expressed has threshold...accessory transposon sequences.  The use of PBx is particularly important to avoid any possible random re-integration...a cell line with the edit of interest. With the larger piggyBac™ donor vector, it is possible to make ...recombinase. However, the use of negative selection largely overcomes this obstacle. Furthermore, we have found...

...Resources on Addgene.org Molecular Biology Reference Pages Basic Molecular Biology Protocols Diagnostic...feature detection with an easy to use interface. We regularly use Snapgene for our quality control process because...will confirm for us is whether it is a linear or circular sequence. Next, we use the “Detect Common Features...analyzing deposits with many plasmids that contain similar features and it allows you to more quickly identify...annotated in our plasmid maps, including our most popular primers and certain restriction enzymes, but you...

...Drawings is similar to using PowerPoint to draw figures. It’s part of Google Drive so it has a similar interface...figures quickly. Inkscape Inkscape is the most similar to Adobe Illustrator out of the three options for...like lab meeting presentations. You can download larger images without the watermark, which are better ...four main categories: anatomy and the human body, cellular biology, medical specialities, and general items... tools settings let you tailor your search to particular types of images. For example, by selecting “Tools...

...scientific sharing worldwide but a select few are particularly special. Over 1000 of our 60,000+ deposited ... the way in which we share plasmids and other molecular tools with the scientific community worldwide,...disseminates throughout the research community. These popular plasmids are categorized into a 3-level system ...can be a time-consuming chore for labs that have popular plasmids but lack the resources or personnel to...our plasmids via Addgene. Given the increasing popularity of CRISPR in the plant community, this has saved... as a whole. To all of our depositors- and in particular our Blue Flame Award winners- we’d like to thank...