We narrowed to 955 results for: ARC;
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TypeBlog Post...greatest’ variants with the best properties for your research. But be aware that the fluorescent proteins reported...under the conditions that are relevant for your research. This will reveal the performance of fluorescent...
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RaPID Detection of RNA-protein Interactions
TypeBlog Post...coli biotin ligase BirA* from BioID and allows a researcher to identify proteins that bind an RNA motif of...biotinylated background proteins reported by other research groups that performed BirA*-based affinity purification... -
dTAG - You're it!
TypeBlog Post...without disrupting protein function. A literature search can help determine which terminus is favorable ...the ENL YEATS domain in acute leukemia. Nature. March 9 2017. 543(7644): 270-274. PubMed PMID: 28241139... -
Antibodies 101: Affinity Reagents
TypeBlog Post...and bind proteins. They’re used in a number of research and clinical applications. Most of the time, when...You never know what problems will arise in your research or what data you may need to collect. Understanding... -
Viral Vectors 101: An Introduction to AAV
TypeBlog Post...make room for a gene of interest, AAVs used in research have their genomes “gutted”, meaning that all ...trunk” space for genetic cargo. Typically, when researchers speak about AAVs, they are actually referring... -
All in a Twist: dsRNA
TypeBlog Post...to sense their formation, and how clinicians, researchers, and even farmers are harnessing these molecules...the introduction of dsRNA. Synthetic dsRNA Researchers have been harnessing the power of dsRNA for a... -
Visualizing Protein Turnover In Situ
TypeBlog Post...former Caltech researcher, now Director at the Max Planck Institute for Brain Research in Frankfurt am... -
Addgene's Tips for Plasmid Quality Control
TypeBlog Post... carefully track samples through our lab using barcoded tubes and plates. The critical pieces of information... to Verify Your Plasmid Learn How Addgene Uses Barcodes to Track Samples Resources on the Addgene Website... -
Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells
TypeBlog Post...refractory to gene delivery methods, but the researchers was able to edit hiPSCs, mouse bone marrow (BM...plasmids. These results are in line with previous research showing that the method of Cas9 and gRNA delivery... -
Hot Plasmids - October 2020
TypeBlog Post...excitatory synapses or that are Cre-inducible, researchers can explore synaptic integration and connectivity...fluorescence. Image from Eastman et al., 2020. The researchers tested several scenarios from embryo development... -
Plasmids for Endogenous Gene Tagging in Human Cells
TypeBlog Post...gleaned from the literature and engagement with researchers who study the protein or structure of interest...introducing tags into your gene of interest. Researchers have developed a variety of endogenous gene tagging... -
Quickest Way to Deposit Plasmids: The Deposit Spreadsheet
TypeBlog Post...This post was originally written by Tyler Ford in March 2017 and updated by Angela Holmes and Christina ...Addgene Blog Find Articles Citing Addgene Plasmids Searchable and Sortable gRNAs for Your Next CRISPR Experiment... -
Overcoming the Challenges of Lentiviral Production
TypeBlog Post...selection and expression in your target cell line, researchers often find themselves faced with a roadblock....cell line that is used for titering. Typically researchers will choose a relatively permissive cell line... -
Educational Planarian Tools for Teaching Developmental Biology Techniques
TypeBlog Post...the number of genes that other classrooms and researchers can easily investigate in planarians. References...Alvarado, The American Biology Teacher, Vol. 79 No. 3, March 2017; (pp. 208-223). Link: http://abt.ucpress.edu... -
Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools
TypeBlog Post...CIB1 domains to separate proteins of interest, a researcher can cause them to interact by stimulation with...decaffeination operon Article contributed by Angela Kaczmarczyk Listen to the synthetic decaffeination operon... -
Lab Automation at Addgene
TypeBlog Post...ever do! However, there is quite a bit of upfront research you have to do to find said robot and to find ...Biology Tips from the Repository Trenches: Using Barcodes to Track Samples Resources at Addgene.org Find... -
Hot Plasmids February 2024
TypeBlog Post...fluorescent proteins derived from StayGold. ResearchSquare (Preprint). https://doi.org/10.21203/rs.3.rs... brilliance By Hridaya Patel (Addgene co-op) Researchers in the Gradinaru lab have developed an engineered... -
CRISPR in the Clinic
TypeBlog Post...mutant allele. When all was said and done, the researchers had cured 24 mice of their disease. Meanwhile...traditional gene therapy pipelines (viral), and researchers are currently working on giving this treatment... -
Viral Vectors 101: Calcium Sensors
TypeBlog Post...targeting. By employing various AAV serotypes, researchers can precisely direct the calcium sensor to specific...complex brain circuit dynamics in vivo. Neuroscience Research. 2021 Aug 1;169:2-8. Suzuki J, Kanemaru K, Iino... -
A Look at Addgene's QC Process
TypeBlog Post...the accumulation of errors in DNA sequences. In research, these errors can affect experimental processes...importance of QC Plasmids are a fundamental part of research. They are easy to produce, easy to edit, and easy...