We narrowed to 1,041 results for: ins
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TypeBlog Post... technologies were without controversy, and it remains to be seen how popular any one of them will become... though a little confusing to the uninitiated, contains much of the information needed to understand how...
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Science Communication for Everyone
TypeBlog Post...sessions led by scientists, which is hosted on our Instagram channel every Sunday. What's planned for the upcoming...participate in our one-hour Live Q&A session on Instagram every Sunday. Undergraduate and graduate students... -
Microbes: Look, Listen, and Tell
TypeBlog Post...loads of inspiration for the current and the next generation of microbiologists. If you got inspired to produce... -
Save Time and Money by Making Your Own Competent Cells
TypeBlog Post...protocol has worked well for us for multiple E. coli strains, including DH5alpha, XL1 Blue, TOP10, ccdB survivalTM...competent cells, then you're best off following the instructions that came with them. If you decided to make ... -
Let There Be LITE Plasmids
TypeBlog Post...lab at MIT, the use of customizable DNA-binding domains based on transcription-activator-like effectors...control gene expression in mouse neurons and in the brains of living mice both quickly and significantly. ... -
What's New in CRISPR - December 2019
TypeBlog Post...bionanoparticle system for packaging Cas9/gRNA ribonucleoproteins for transient expression. They replaced the...ADAR2 into a cytidine deaminase. This editor still retains A-to-I editing. This system is called RNA Editing... -
CRISPR Kinome Libraries Available: Pooled and Individual Plasmid Formats
TypeBlog Post...the Genetic Perturbation Platform at the Broad Institute, John Doench. A genetic screening project can ...still offering the potential for true biological insight. Pooled and individual plasmid formats A first ... -
Modulate the Activity of 17 Signaling Pathways with One Kit!
TypeBlog Post...Kathleen Ottina and David Sabatini at the Whitehead Institute and MIT, started with a list of 17 signaling pathways...pathways. With support from the National Cancer Institute, they expect to add another 200 or 250 constructs... -
Management for Scientists: Seeking Feedback
TypeBlog Post...series on Management for scientists. This second installment in the Management for Scientists series will ...Walking Around" – don't hide in your office. Stop by instead of emailing. Demonstrate you are open to feedback... -
X-CHIME: Context Dependent Germline Knockout in Immune Cells
TypeBlog Post...suite of four systems which utilizes custom mouse strains and lentiviral expression vectors (Table 1). The...generation of exhausted CD8+ T cell subpopulations and restrains tumor immunity. Nature Immunology 20, 1335–1347... -
SciComm with the Experts at Science in the News Part 2
TypeBlog Post...Maintaining Institutional Knolwedge and Continuity - SITN's key technique for retaining institutional knowledge... -
Searchable and Sortable gRNAs for Your Next CRISPR Experiment
TypeBlog Post...develop a searchable and sortable datatable that contains validated gRNA sequences. Validated in this context...to deliver your CRISPR components as RNPs (ribonucleoproteins) and therefore usually transcribe your gRNAs... -
CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]
TypeBlog Post...CRISPR/Cas9 for targeted genomic deletions We were inspired to produce intrachromosomal deletions based on...copy at a given locus may be useful to study haploinsufficiency. Subsequent modifications at the remaining... -
"What Makes a Good Mentor?" and 6 More FAQs About Science Mentoring
TypeBlog Post... own.” “Someone to act as your ‘cheerleader’ – inspire you.” “Also a mentor can give you realistic expectations...mentor does all of these things and more. It is instructive (and entertaining) to review this list of bad... -
2023 and 2024: Reflections and Looking Ahead
TypeBlog Post...June, we announced a new partnership with the Institute for Protein Innovation (IPI), in which Addgene.... The first IPI collection, 31 rAbs targeting integrins, will be released early this year. In September... -
Cre-ating New Methods for Site-specific Recombination in Drosophila
TypeBlog Post...Kotelevtseva N, King K, Davis JR, Mullins LJ, Chapman KE, Dunbar DR, Mullins JJ. Nucleic Acids Res. 2007;35... -
London Calling - Addgene’s New Europe Office in London
TypeBlog Post...75,000 plasmids to more than 1,500 different institutions in Europe. In the last four years alone, the...fantastic numbers, a lot of European scientists and institutions are not aware of what Addgene can do for them... -
New Viral Vectors - Fall 2024
TypeBlog Post...If the fall colors are inspiring you to paint your cells in equally vivid colors, you'll want to check...vectors from the Jonathan Ting lab at the Allen Institute, deposited as part of the BRAIN Armamentarium ... -
An Addgene Summer Internship
TypeBlog Post...toxin, or an oncogene, for safety reasons, and the insert size should be a max of 500 bp (preferably). I ...college and play the drum set as their personal instrument. They are the oldest sibling in a Guatemalan ... -
New CRISPR Web Resources and #12DaysofCRISPR Recap
TypeBlog Post...demethylation by Tet1. RNA Targeting: This page contains Cas13 constructs for mammalian and bacterial systems...off-target effects! This characteristic probably explains why pC016 - LwCas13a guide expression backbone...