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  1. Management for Scientists: Delegating is Key

    Type
    Blog Post
    ...Medical School researcher Connie Cepko to learn about her management style. Recipes for delegation success...accountability? What types of issues do you want to know about right away? Provide adequate support. The manager...

  3. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ...targets compared to Cas9.  Read on to learn more about Cpf1 multiplexing.  For an in-depth review of Cpf1... Addgene. If you have any questions or thoughts about Cpf1 multiplexing, leave them in the comments below...

  4. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    ...advantages over mismatch nuclease assays that will be elaborated below. Digital PCR Digital PCR (dPCR) involves...type targets. Comparatively, detection limits of about 5% mutant targets have been reported for mismatch...

  6. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    ...vector name and serotype you’d like to be notified about. Chemogenetics Plasmid Serotype Name 44362 ...vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols and...

  7. Viral Vectors 101: Biosensors

    Type
    Blog Post
    ...component of a biosensor is the thing that you care about within your cellular or animal system. This ‘thing...for expression and downstream monitoring. Nervous about making a virus for your biosensor? Don’t be! We ...

  8. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...Viruses as Biological Tools blog can help you learn about the different types of viruses and pick one for ...Addgene.org Browse Our Viral Vector Pages Learn About Viral Vector Biosafety Deposit Your Viral Vectors...

  9. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ... in science brings stronger networking across laboratory and disciplinary boundaries and can allow for... that are otherwise cut off from the process.   About the author: Benedict Diederich received his PhD ...

  12. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    ...packaging methods. The three components described above (envelope, packaging, and transfer) are supplied...gamma-retrovirus.    Frequently asked questions about γ-retroviral vectors How do engineered γ-retroviruses...

  13. AAV Q&A with Tim Miles

    Type
    Blog Post
    ...labeling in the brain with PHP.eB: A recent CLOVER collaboration injected 1e+13 vg per mouse to get phenotypes...coverage. Please see this paper for more information about PHP.eB brain labeling at various doses.  As always...

  17. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    ...questions is the same--methylation! Read on to learn about how DNA methylation may affect your restriction ...be methylated. Using the same example plasmid as above (which has the XbaI site blocked by methylation)...

  18. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...with limited packaging capacity. Still confused about the various tRNA expression strategies? No worries... Addgene. If you have any questions or thoughts about this technology, leave them in the comments below...

  19. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    ... extremely heterogeneous. There is consequently about a two-thirds chance of causing a frameshift mutation...favor accumulation of the latter products. As noted above, a single cycle of cleavage and accurate repair ...

  20. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    ...communities. The ARRIVE guidelines for the reporting of laboratory animals during in vivo research and the CARE ...writing! Let us know if you have any other questions about writing and publishing your manuscript, or add your...
Showing: 701 - 720 of 963 results