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...This post was updated on March 21, 2018. Most of the time, plasmid prepping is a breeze. You get your...copy number of the empty plasmid backbone or a researcher’s personal observations. If you are experiencing...

...If you follow CRISPR research, you know all about using non-homologous end-joining (NHEJ) to make deletions...al.’s use of MMEJ represents another strategy researchers can exploit for CRISPR gene editing. In organisms...

...enabling microscopes to visualize the cellular architecture of whole tissues in 3D with unprecedented detail...A. Dawood, M. Carballosa, et al, (2015) eNeuro, March/April 2015, 2(2) e0001-15.2015. Pubmed. Resources...

...strategies most widely incorporated into plasmids for research purposes are described below. IRES Elements Translation...to plasmids, allowing you to spend less time researching plasmid basic features and spend more time developing...

...the Future Initiative. Endy's team made news in March with a publication in Science describing the development...translation initiation." Nature Methods. 10, 354-360 (10 March 2013). ...

...Molecular Biology. Jake Watson is a postdoctoral researcher in neurophysiology at the MRC Laboratory of Molecular...homologous recombination in vivo." Nucleic acids research 21.15 (1993): 3601. PubMed PMID: 8346047. PubMed...

...structure (Figure 1). Here, MXS Chaining gave researchers the ability to generate the four initial unique...acid. The Journal of biological chemistry. 1961. March;236:864–875. Pubmed PMID: 13790780. 5. Shetty RP...

...nm peak is more specific to proteins and why researchers traditionally use the A260/280 ratio as an indicator...Qiagen Gene Expression Newsletter. Issue 15/10. March 2010. https://www.qiagen.com/de/~/media/1ea8aec3bfa24543a28fcaea25986514...

...derivatives are a staple in the lab. GFP is used in research across a vast array of biological disciplines ...protein and facilitating its widespread use in research. Many other mutations have since been introduced...

...commentary on their application to neuroscience research. What is CRISPR-based activation? To summarize...immunohistochemistry, it is not amenable to FACS.  Thus, the researchers inserted human CD8, a gene that encodes a surface...

...→ office culture shock? Working in an academic research lab involves a few key features that can be difficult...community (and sometimes conduct their own independent research!). There are also organizations like Science from...

...importance of bioinformatics at Addgene and how our search for solutions led us to a partnership with Seqera... programs to a uniform language.  The modern architecture of Nextflow included built-in support for containers...

...A recent survey of PhDs found that many researchers feel they lack formal training in a variety of transferable...'re helping to fill this gap by both helping researchers recognize their MANY transferable skills learned...

...polyethyenimine transfection protocol. I also recommend searching PubMed for any publications associated with the...feel more confident incorporating them into your research. And, of course, if you have any questions, don...

...pathways and spontaneous mutations.  Here, the researchers were able to target multiple genes in M. abscessus...catalyze the luminescence reaction (Figure 5). The researchers used the reporter to visualize biologically relevant...

...target!) and use them in numerous fields including research and development, diagnostics, drug discovery, ...interfere with conjugates. Make sure to do your research before conjugating at the bench.   Indirect ELISAs...

...Coastal & Estuarine Research Federation (CERF) Biennial Conference, Gordon Research Conference, and the...

...pathogen more safely. Recently, many SARS-CoV-2 researchers began pseudotyping the SARS-CoV-2 spike protein...Improve Gene Modification of Hematopoietic Cells for Research and Gene Therapy. Viruses 12:1016 . https://doi.org...

... children and patients with skin disorders for research and clinical trials, as it allows access to a ... fellow at Massachusetts General Hospital with research interests in the fields of gene editing and stem...

... 16 labs around the world and can be used by researchers to validate their qPCR assay (Lock et al., 2010...titrating AAV vectors." Medical science monitor basic research19 (2013): 187. PubMed PMID: 23828206. PubMed Central...