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Showing: 701 - 720 of 1006 results
  1. Addgene Depositors Get More Citations

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    Blog Post
    ...Microbiology, Biochemistry & Molecular Biology, and Biochemical Research Methods. We also pulled the big interdisciplinary...the papers with plasmids deposited at Addgene – typically about four times as many as papers without plasmids... of publication. Table 1 provides an example calculation of the weights. Table 1: Journal Weighting Example...
  2. Trading Spaces - Visiting Scientist Edition

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    Blog Post
    ...the host university does not hire or pay them (typically). A visiting scientist is distinct from equipment...the scientist to the new institute where their typical workdays will be carried out until the end of the...one) to communicate your plan as well. Making logistical and funding arrangements Visiting scientist positions...
  3. What Good Citizenship Can Do for Reproducibility in Science

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    Blog Post
    ...for actually making it happen would increase dramatically. I’m not sure what the best approach to doing...at The Scripps Research Institute in La Jolla, California. She joined Elsevier after her postdoctoral studies...primary research and review journals across life and physical science, and editorial contribution to the strategic...
  4. Hot Plasmids - January 2023

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    Blog Post
    ...30667360; PMCID: PMC6377228. Hyperfolder YFP- a chemically stable tool for microscopy by: Susanna Stroik...many of these applications have lagged. Fixation, chemical staining, and light exposure can negatively affect...Find YFP plasmids here!   Campbell, B.C., et al. Chemically stable fluorescent proteins for advanced microscopy...
  5. AAV Q&A with Tim Miles

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    Blog Post
    ...Tim Miles, PhD, Director of the CLOVER Center at CalTech? If so, you may have submitted a question that ...Have you or others been able to successfully systemically transduce and then retransduce a model organism...as multiple sclerosis?  A: Basic science and preclinical researchers have used the flexibility of AAV ...
  6. Antibodies 101: Isotypes

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    Blog Post
    ...chain constant regions result in several unique physical characteristics of these five isotypes, such as...their heavy chain constant domains. These are typically numbered, i.e. IgG1, IgG2, etc. Let’s dive in ...different constant domain gene. This process is called isotype switching and results in the B cell expressing...
  7. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

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    Blog Post
    ...study it. The process is fairly straightforward. Basically, you place your gene of interest in a plasmid ...genetic crosses with balancer chromosomes to make genetically stable fly lines. After a few weeks of waiting... aware of at least some of the possibilities. Basically, if you can devise an experimental assay that ...
  8. Addgene’s Viral Service - Why Virus? Why Now?

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    Blog Post
    ...North Carolina, Connie Cepko’s lab from Harvard Medical School, and Jonathan Ting’s lab from the Allen ...educational materials, and provides viral vector technical service (details about virus production protocols...word out about this new resource and providing fantastically useful educational content on viral vectors....
  9. Clear and Accessible Writing

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    Blog Post
    ... reader accessing the information? This can be physical or digital. For instance, you would present information...most helpful to use a lot of jargon and have a technical, detailed approach. Other times, it is more helpful...thought it would be most helpful to highlight the technical details of collecting the purified AAV–that the...
  10. Viral Vectors 101: Inverted Terminal Repeats

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    Blog Post
    ...Adeno-associated viruses (AAV) are an important biological tool that allow scientists to express a gene ...with biorender.com. ITR structure  WT ITRs are symmetrical 145-nucleotide sequences that flank the ends ...serotypes vary in their gene transfer efficiency. Our practical knowledge of ITRs is still limited, but this area...
  11. Behind the scenes: Addgene’s new search engine and more

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    Blog Post
    ..., a powerful open-source search engine that can scale to search millions of web resources and handle millions..., partial words, phrases, partial phrases, or numerical identifiers. We also combine the different terms...PI labs and institutions. There are many more technical details that make our new search engine unique...
  12. Plasmids 101: Methylation and Restriction Enzymes

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    Blog Post
    ...restriction endonucleases. A given prokaryote typically has genes encoding one or a few restriction modification...Well, even though Dam methylation sites are not specifically associated with any restriction modification... These specialized E. coli strains have been specifically engineered to be Dam and Dcm methylase-deficient...
  13. Human Germline Editing Using CRISPR

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    Blog Post
    ... rate would need to be much, much lower before clinical applications could be considered. It is important... efficiency and applicability. Given moral and ethical concerns surrounding such research, it is crucial... experiments are needed before we can consider clinical applications for germline editing. Although this...
  14. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

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    Blog Post
    ...developed cloning vectors to harness it. These vectors, called pKIL18 and pKIL19, contained the ccdB gene in frame...ccdB and therefore would die. This procedure dramatically reduces the number of clones that do not contain...and ccdB Survival strains have been developed specifically with ccdB-containing vectors in mind, any plasmid...
  15. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

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    Blog Post
    ...reprograming influence tumors development? Being pathological active regions, microenvironments shape a tumor...interested in science journalism and writing about biomedical issues. Follow her on Twitter @BrbaraPinho6. ...expression predicts early tumor recurrence and poor clinical outcome in human breast cancers." The American...
  16. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

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    Blog Post
    ... year, narrow by article type (review, patent, clinical study, research article etc). There are also numerous...conclusions. In any case, the reader should give a critical eye to all results in the relation to how they... biochemistry instructor in the Department of Biological Sciences at Louisiana State University. She is...
Showing: 701 - 720 of 1006 results