We narrowed to 802 results for: Clu
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TypeBlog Post...transformation method. The general steps for this method include: Cloning and transforming a plasmid into the bacterium...
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AAVs in Retinal Gene Therapy
TypeBlog Post...identification of several disease-causing genes, including the RPE65 gene responsible for an autosomal recessive... -
INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons
TypeBlog Post...various gram-negative bacterial hosts of interest, including within mixed communities and into species not ... -
CRISPR Antimicrobials
TypeBlog Post... multiple approaches. Major restaurant chains including Chipotle and Panera Bread have pledged to eliminate... -
Cpf1: A New Tool for CRISPR Genome Editing
TypeBlog Post...Cpf1 found and tested? Class 2 CRISPR systems, including the type II Cas9-based system, require a single-component... -
Tips from the Repository Trenches: Using Barcodes to Track Samples
TypeBlog Post...assigning a physical location to your samples, include as much detail about that location as possible.... -
Negotiating Work and Life: How to Find the Joy
TypeBlog Post...people in the same situation. Go to church, join a club, meet the neighbors and create a network of support... -
Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research
TypeBlog Post...PEG-mediated transformation into plant cells. Conclusion & Future Directions TnpB represents a new generation... -
Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)
TypeBlog Post... your cloning. Most sequence analysis software includes Golden Gate cloning tools that simulate the restriction–ligation... -
Twenty Years of Sharing: Expanding Accessibility
TypeBlog Post...systemic changes, like using alt-text, captions, and inclusive design in our digital content. We have implemented... -
Plasmids 101: Stringent Regulation of Replication
TypeBlog Post...process. When enough copies of ATP-DnaA are bound, including at the low-affinity sites, the proteins work cooperatively... -
Hot Plasmids - November 2023
TypeBlog Post...about the whole-brain reference atlas efforts, including a list of validated antibodies, on their webpage... -
Transferable Skills Guide: Creativity
TypeBlog Post...dissenting opinions is to foster a culture of inclusivity. Work with and value people who think differently... -
Viral Vectors 101: Pseudotyping
TypeBlog Post..., Bieniasz PD, Jäger U, Porter CD, Friedman T, McClure MO, Weiss RA (1997) Sensitization of rhabdo-, lenti... -
Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone
TypeBlog Post... from many with experience mentoring in STEM, including Addgene’s Joanne Kamens. Expanding Binning Singletons... -
Transferable Skills Guide: External Collaborations
TypeBlog Post...Get sponsors for a training weekend for a sports club, find speakers for a meetup or recruit volunteers... -
Lab to Office Culture Shock
TypeBlog Post...whether you realize it or not. These skills might include collaborating, managing, and communicating. After... -
Supporting Reproducibility with a Connected ELN
TypeBlog Post... use of other tools they use in research. This includes chat apps like Slack, Microsoft Teams, and Google... -
Changing Labor Laws Bring Increased Postdoc Wages
TypeBlog Post...administered, which leads to many complications, including the inability of some institutions to know exactly... -
Neuronal labeling with Spaghetti Monster
TypeBlog Post...smFPs can result in better resolution images. Conclusion By combining advantages of FPs and epitope tags...