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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 967 results for: Cre

Showing: 701 - 720 of 967 results

  1. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    ...empirical essays had also stirred some of my more creative faculties into motion, such that a desire to once...filmmaking, podcasting, philosophy of science, creative writing, designing and curating exhibitions, animation...

  2. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ...different biochemical secondary energy sources to increase protein yields from 36 μg/mL to 266 μg/mL. The...oxytetracycline enzymes. Next, this system was used to recreate natural biosynthetic pathways. Biosynthetic pathways...

  3. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

    Type
    Blog Post
    ...out-of-the-box ideas that might jump start your learning. 1. Create a peer mentoring group We don’t all get the mentoring...that there’s some correct level of intimacy that creates the magic of a safe space. No matter why, now is...

  5. Plasmid Cloning by PCR

    Type
    Blog Post
    ...temperatures, but you may need to increase your primer length and increase the Tm if you are trying to clone...

  7. Mentoring for Scientists: I Have a Mentor, What Now?

    Type
    Blog Post
    ...her mentoring style. Set goals with a mentor: The secret of accountability To make a mentoring relationship...perspectives Develop coaching skills Encourage your creative thinking Enhance leadership skills Support inclusion...

  8. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    ...across the hall, fields, and other professions increases your chances of finding a well-suited job. EAs...of qualified scientists entering the workforce increases, EAs allow graduate students and postdocs an opportunity...

  9. Celebrate National Book Month with Addgene

    Type
    Blog Post
    ...conclusion? In this series, there is always another secret."     - Marcy Patrick, Associate Director of Quality...something even more sinister. 1000 pages long. Incredibly imaginative, funny, bittersweet, and shocking...

  12. DAP arrays for multiplex gene editing

    Type
    Blog Post
    ...potential in vivo applications or multiplex CRISPR screening.   Using tRNAs Luckily, a fun feature of tRNA...engineering of crops or allow for sophisticated CRISPR screenings that would perturb the unprecedented number of...

  13. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    ...genetic diseases As CRISPR technology becomes increasingly more cost effective and precise, researchers...influence editing efficiency. In 2022, despite the increasing effectiveness of CRISPR/Cas9, scientists are ...

  15. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    ...easier. NEBNext Ultra FS DNA Library Prep Kit To create a DNA library, one must first fragment a DNA sample...snacks. At the end of the workshop, all our newly created libraries were analysed to see how well the kit...

  17. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ...translationally silent. Both of these methods increase our understanding of translation dynamics, but...but they come with a few caveats. In both cases, creating a reporter mRNA requires inserting multiple hairpin...

  18. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    ...advances in technologies that permit large-scale creation and synthesis (“writing”) of longer pieces of ...samples from several plates are pooled together to create a single library containing fragments carrying ...

  19. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    ...mutagenesis to extend at different pH values. Using a screen that takes advantage of the fact that coiled R-...both lower and higher pH. We initially used this screen to find R body mutants that couldn’t extend at ...

  20. Components of CRISPR/Cas9

    Type
    Blog Post
    ...xCas9 is a variant with increased PAM flexibiliy and eSpCas9/SpCas9-HF1 have increased targeting specificity...
Showing: 701 - 720 of 967 results