We narrowed to 958 results for: Cre

...easier. NEBNext Ultra FS DNA Library Prep Kit To create a DNA library, one must first fragment a DNA sample...snacks. At the end of the workshop, all our newly created libraries were analysed to see how well the kit...

...translationally silent. Both of these methods increase our understanding of translation dynamics, but...but they come with a few caveats. In both cases, creating a reporter mRNA requires inserting multiple hairpin...

...advances in technologies that permit large-scale creation and synthesis (“writing”) of longer pieces of ...samples from several plates are pooled together to create a single library containing fragments carrying ...

...potential in vivo applications or multiplex CRISPR screening.   Using tRNAs Luckily, a fun feature of tRNA...engineering of crops or allow for sophisticated CRISPR screenings that would perturb the unprecedented number of...

...genetic diseases As CRISPR technology becomes increasingly more cost effective and precise, researchers...influence editing efficiency. In 2022, despite the increasing effectiveness of CRISPR/Cas9, scientists are ...

...inactivate the drug and increase drug clearance, lowering its efficacy and increasing the risk of an adverse...

...two iodine residues. Compared to MG, MG-2I has increased ROS generation when bound by the dL5** FAP.  The... KillerRed requires mitochondrial targeting to increase cell ablation efficiency, but dL5** phototoxocity...

...mutagenesis to extend at different pH values. Using a screen that takes advantage of the fact that coiled R-...both lower and higher pH. We initially used this screen to find R body mutants that couldn’t extend at ...

...xCas9 is a variant with increased PAM flexibiliy and eSpCas9/SpCas9-HF1 have increased targeting specificity...

...harness the power of light, Christopher Voigt’s lab created the first E. coli light-sensitive two-component...pathways. They also reduced system leakiness and increased dynamic range to around 100-fold. These improvements...

...leadership roles leads to more ideas, initiative and creative thinking. The team members will be more invested...Organization Can Help You Develop Leadership Skils Set Concrete Goals with Your Mentor through an Individual Development...

...time-intensive, so we recommend a variety of ways to screen and verify your plasmids. Here, we'll cover restriction...500bp to 10kb, with the 3.0kb fragment having increased intensity to serve as a reference band. The uncut...

...next-gen sequencing (NGS), and high-throughput screening. While some developers had science backgrounds...solution is more complicated. You’ll need to be creative to be able to work with others to come up with...

...genes with plasmids on Addgene.org AGO1 BRCA1 CREBBP CUL1 H2AFX H2AFY H3F3A H3.1 H3.3 HIST1H1E HIST1H2BB...Gerlich DW Cell. 2017 Aug 24;170(5):956-972.e23 Screen identifies bromodomain protein ZMYND8 in chromatin...

...Immuno-Oncology”) is exploding. Researchers are increasingly able to harness the body’s immune system to ...microenvironment Tumor Immunosurveillance and Defense With increasingly sophisticated visualization techniques it is...

...the number of fluorescent proteins is steadily increasing. Often, the choice for a fluorescent protein ...of a fluorescent molecule. A higher brightness increases the detected signal and therefore high brightness...

... or phosphate molecules of the RNA backbone to increase stability.  Nature has evolved ways to prevent...surprisingly well-tolerated by the Cas9 protein and can increase on-target efficiency. In the same vein, locked...

... recognition site for proviral DNA synthesis. Increases transduction efficiency and transgene expression...that stimulates the expression of transgenes via increased nuclear export. LTR Subcomponents: -U3 -...

... college teaching experience, Kuldell sought to create a program that would capitalize on the excitement...to SynBio Assembly Standards Find Plasmids for Creating and Controlling Gene Networks Browse All SynBio...

...must be utilized or a new combination needs to be created in advance of the experiment. The marker provided...physiological levels due to high copy numbers. This creates a potential metabolic burden on the yeast cells...