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We narrowed to 958 results for: Cre

Showing: 701 - 720 of 958 results
  1. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    ...easier. NEBNext Ultra FS DNA Library Prep Kit To create a DNA library, one must first fragment a DNA sample...snacks. At the end of the workshop, all our newly created libraries were analysed to see how well the kit...
  2. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ...translationally silent. Both of these methods increase our understanding of translation dynamics, but...but they come with a few caveats. In both cases, creating a reporter mRNA requires inserting multiple hairpin...
  3. The Power Behind NGS Plasmid Validation: seqWell

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    Blog Post
    ...advances in technologies that permit large-scale creation and synthesis (“writing”) of longer pieces of ...samples from several plates are pooled together to create a single library containing fragments carrying ...
  4. Celebrate National Book Month with Addgene

    Type
    Blog Post
    ...conclusion? In this series, there is always another secret."     - Marcy Patrick, Associate Director of Quality...something even more sinister. 1000 pages long. Incredibly imaginative, funny, bittersweet, and shocking...
  5. R Bodies: Membrane-Rupturing Microscopic Tools

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    Blog Post
    ...mutagenesis to extend at different pH values. Using a screen that takes advantage of the fact that coiled R-...both lower and higher pH. We initially used this screen to find R body mutants that couldn’t extend at ...
  6. Components of CRISPR/Cas9

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    Blog Post
    ...xCas9 is a variant with increased PAM flexibiliy and eSpCas9/SpCas9-HF1 have increased targeting specificity...
  7. Synthetic Photobiology: Optogenetics for E. coli

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    Blog Post
    ...harness the power of light, Christopher Voigt’s lab created the first E. coli light-sensitive two-component...pathways. They also reduced system leakiness and increased dynamic range to around 100-fold. These improvements...
  8. Management for Scientists: Delegating is Key

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    Blog Post
    ...leadership roles leads to more ideas, initiative and creative thinking. The team members will be more invested...Organization Can Help You Develop Leadership Skils Set Concrete Goals with Your Mentor through an Individual Development...
  9. Giving gRNAs a Facelift - Synthetic and Beyond

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    Blog Post
    ... or phosphate molecules of the RNA backbone to increase stability.  Nature has evolved ways to prevent...surprisingly well-tolerated by the Cas9 protein and can increase on-target efficiency. In the same vein, locked...
  10. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    ...next-gen sequencing (NGS), and high-throughput screening. While some developers had science backgrounds...solution is more complicated. You’ll need to be creative to be able to work with others to come up with...
  11. Viral Vectors 101: Gamma-Retroviral Packaging Systems

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    Blog Post
    ... recognition site for proviral DNA synthesis. Increases transduction efficiency and transgene expression...that stimulates the expression of transgenes via increased nuclear export. LTR Subcomponents: -U3 -...
  12. A Deep Dive into BioBuilder

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    Blog Post
    ... college teaching experience, Kuldell sought to create a program that would capitalize on the excitement...to SynBio Assembly Standards Find Plasmids for Creating and Controlling Gene Networks Browse All SynBio...
  13. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    ...must be utilized or a new combination needs to be created in advance of the experiment. The marker provided...physiological levels due to high copy numbers. This creates a potential metabolic burden on the yeast cells...
Showing: 701 - 720 of 958 results