We narrowed to 957 results for: Cre
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TypeBlog Post...across the hall, fields, and other professions increases your chances of finding a well-suited job. EAs...of qualified scientists entering the workforce increases, EAs allow graduate students and postdocs an opportunity...
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Predicting Adverse Reactions to Monoclonal Antibody Drugs
TypeBlog Post...inactivate the drug and increase drug clearance, lowering its efficacy and increasing the risk of an adverse... -
Learning Technical Skills at a Lab Central Workshop
TypeBlog Post...easier. NEBNext Ultra FS DNA Library Prep Kit To create a DNA library, one must first fragment a DNA sample...snacks. At the end of the workshop, all our newly created libraries were analysed to see how well the kit... -
Celebrate National Book Month with Addgene
TypeBlog Post...conclusion? In this series, there is always another secret." - Marcy Patrick, Associate Director of Quality...something even more sinister. 1000 pages long. Incredibly imaginative, funny, bittersweet, and shocking... -
Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**
TypeBlog Post...two iodine residues. Compared to MG, MG-2I has increased ROS generation when bound by the dL5** FAP. The... KillerRed requires mitochondrial targeting to increase cell ablation efficiency, but dL5** phototoxocity... -
Visualizing Translation at the Single Molecule Level
TypeBlog Post...translationally silent. Both of these methods increase our understanding of translation dynamics, but...but they come with a few caveats. In both cases, creating a reporter mRNA requires inserting multiple hairpin... -
The Power Behind NGS Plasmid Validation: seqWell
TypeBlog Post...advances in technologies that permit large-scale creation and synthesis (“writing”) of longer pieces of ...samples from several plates are pooled together to create a single library containing fragments carrying ... -
DAP arrays for multiplex gene editing
TypeBlog Post...potential in vivo applications or multiplex CRISPR screening. Using tRNAs Luckily, a fun feature of tRNA...engineering of crops or allow for sophisticated CRISPR screenings that would perturb the unprecedented number of... -
Using CRISPR/Cas9 to Edit Disease Out of the Genome
TypeBlog Post...genetic diseases As CRISPR technology becomes increasingly more cost effective and precise, researchers...influence editing efficiency. In 2022, despite the increasing effectiveness of CRISPR/Cas9, scientists are ... -
The Crispr Babies Saga Shows the Need for Action, Not More Delays
TypeBlog Post...in China was using CRISPR/Cas9 in an attempt to create gene-edited babies. Several hours later, the scientist...the media and academic conference response to He created a positive feedback loop that rewarded unethical... -
Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish
TypeBlog Post...such as pACBSR-hyg and pFLP-hyg will be used to create gene deletions of important unknown regulatory ...sense contaminants and emit light as a response. To create this bacterial sensor, he will construct plasmids... -
R Bodies: Membrane-Rupturing Microscopic Tools
TypeBlog Post...mutagenesis to extend at different pH values. Using a screen that takes advantage of the fact that coiled R-...both lower and higher pH. We initially used this screen to find R body mutants that couldn’t extend at ... -
Components of CRISPR/Cas9
TypeBlog Post...xCas9 is a variant with increased PAM flexibiliy and eSpCas9/SpCas9-HF1 have increased targeting specificity... -
Synthetic Photobiology: Optogenetics for E. coli
TypeBlog Post...harness the power of light, Christopher Voigt’s lab created the first E. coli light-sensitive two-component...pathways. They also reduced system leakiness and increased dynamic range to around 100-fold. These improvements... -
Management for Scientists: Delegating is Key
TypeBlog Post...leadership roles leads to more ideas, initiative and creative thinking. The team members will be more invested...Organization Can Help You Develop Leadership Skils Set Concrete Goals with Your Mentor through an Individual Development... -
Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis
TypeBlog Post...time-intensive, so we recommend a variety of ways to screen and verify your plasmids. Here, we'll cover restriction...500bp to 10kb, with the 3.0kb fragment having increased intensity to serve as a reference band. The uncut... -
Viral Vectors 101: Gamma-Retroviral Packaging Systems
TypeBlog Post... recognition site for proviral DNA synthesis. Increases transduction efficiency and transgene expression...that stimulates the expression of transgenes via increased nuclear export. LTR Subcomponents: -U3 -... -
Why and how to start studying chromatin (with Addgene's help)
TypeBlog Post...genes with plasmids on Addgene.org AGO1 BRCA1 CREBBP CUL1 H2AFX H2AFY H3F3A H3.1 H3.3 HIST1H1E HIST1H2BB...Gerlich DW Cell. 2017 Aug 24;170(5):956-972.e23 Screen identifies bromodomain protein ZMYND8 in chromatin... -
A Quick Guide to a Career in Software Product Management
TypeBlog Post...next-gen sequencing (NGS), and high-throughput screening. While some developers had science backgrounds...solution is more complicated. You’ll need to be creative to be able to work with others to come up with... -
Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer
TypeBlog Post...Immuno-Oncology”) is exploding. Researchers are increasingly able to harness the body’s immune system to ...microenvironment Tumor Immunosurveillance and Defense With increasingly sophisticated visualization techniques it is...