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We narrowed to 1,033 results for: ELL

Showing: 701 - 720 of 1033 results

  1. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type
    Blog Post
    ...R-factors could be transferred between bacteria via cell-to-cell contact; however, scientists noted that, unlike...cytoplasmic factors that could be transferred between cells. At the time, these extranuclear agents of heredity...PMCID: PMC3785787. References: Lederberg, Joshua. "Cell Genetics and Hereditary Symbiosis" Physiological...Transmissible Drug Resistance in an Epidemic Strain of Salmonella Typhimurium.” The Journal of Hygiene 60.3 (1962...

  2. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    ...to supplement your cells with biliverdin for maximal brightness. In mammalian cells, one of the improved...Imaging Center at UCSF. A common requirement for live cell imaging experiments is the ability to follow multiple...emission spectra of the protein, so that the protein is well excited by your microscope and the fluorescence ...laboratory of Ronald Vale, after which he was a fellow at the Bauer Center for Genomics Research at Harvard...

  3. Early Career Researcher Toolbox: Social Media for Scientists

    Type
    Blog Post
    ...can also find Dr. Page and Hello PhD on Twitter: @ThePurplePage and @HelloPhD "Role" call. #BLACKandSTEM...Science Communication” at the American Society for Cell Biology (ASCB) 2018 Conference. I don’t exactly ...Scientists SHOULD Be Using Twitter" podcast from Hello PhD In this podcast the hosts, Josh and Dan, discuss...there’s some great reminders for Twitter pros as well, like tip #4: “Follow people for about a week and...

  4. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    ...recognized and valued for its immense importance, it may well be what determines whether you get the job after...as an opportunity to practice communicating.  Excellent scientists learn from advisers and other mentors...forget that you have a much bigger job than merely telling your audience about the work you've done. If you...help graduate students in all STEM fields become excellent science communicators. To empower graduate students...in science communication The strength of story telling Science communication: 9 strategies to get your...

  5. From Lab Bench to Leadership: Transitioning into Management as a PhD

    Type
    Blog Post
    ...team potential. I often thought that performing a cell culture experiment myself would take less time than...Another team member was highly self-motivated and excelled when given the freedom to explore projects autonomously...management skills. Finally, developing emotional intelligence is vital. Learning to "read the room," understanding...part of your broader career development.   Matteo Tardelli authored the book "Beyond Academia: Stories and...molecular biology and metabolism. He now guides fellow PhDs in their careers with actionable steps and...

  6. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    ...stimulate AraC binding to I1 and I2 sites. Supplementing cell growth media with glucose decreases cAMP and represses...Steroid-regulated LexA promoter XVE (synthetic) pFZ19 (yellow flame) Temperature inducible promoters Temperature... Because transcription machinery differs between cell types or organisms, promoters are similarly variable...variable. Bacterial promoters only work in prokaryotic cells and typically only in the same or closely related... were derived. Similarly, the various eukaryotic cell types (mammalian, yeast, plants, etc.) require unique...

  7. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

    Type
    Blog Post
    ... Many thanks to our guest blogger David Mellor! David Mellor leads the policy and incentive programs ...This post was contributed by David Mellor from the Center for Open Science. In the last decade, researchers...Before coming to the Center for Open Science, Dr. Mellor worked with citizen scientists to design and implement...at https://orcid.org/0000-0002-3125-5888 and @EvoMellor.  ...

  8. Will You Be My Mentor? Finding and Asking for Mentoring Support

    Type
    Blog Post
    ...have lunch or coffee with someone I don’t know very well at least twice a week. I have done this with people... career advice. Your family and friends know you well and might have good ideas for who you should connect...Healthcare Businesswomen’s Association hosts an excellent group mentoring program in many of its chapters...keep working with you is report back with progress. Tell them how their advice made a difference, helped ...in a leap in your skills. If a first meeting goes well, you can say, “This was really helpful, perhaps ...

  9. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ...system, antibodies are produced by B cells. Each individual B cell produces antibodies that all recognize...also all the same isotype. But together, all the B cell clones in the immune system make different isotypes...antibodies starts with selecting just one of these B cell clones for further antibody production, polyclonal... blood from the immunized animal. The red blood cells and the serum are separated, and the serum - which...

  10. Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

    Type
    Blog Post
    ...thinking. Being able to learn things quickly. Story telling. The ability to know what’s a story and how to ...to hook your readers with it.” She feels that storytelling is an impactful tool for science communicators...through connections and that’s something that good storytellers in science can do.” Susan’s number one piece... starting a blog, but with one caveat: “I hate telling people to work for free because you shouldn’t work...social media as a scientist here Learn more about storytelling in science ...

  11. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    ...Professional Career Coaching can be an excellent tactic for scientists making a career shift or who wish...of this to get the information you need to choose well. What are their qualifications? Do they have certifications...does not endorse any of these coaches and I can't tell you which one will be a match for your needs, but...Consulting is part of that group and she is especially excellent in coaching for communication skills. Industry...Industry transition is also an area of specialty for Michelle Capes at Adeptify Me. While based in Madison, ...

  12. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...currently doing this using two approaches: single-cell RNA-sequencing to identify neuronal class and deep...Drosophila and zebrafish articles published there as well.   Worm Development Dynamics Database (WDDD) The...WDDD aims to acquire quantitative data on all the cell divisions of the C. elegans embryo when all essential...Wang et al., 2018).” With the split system, only cells that have both halves of cGAL can drive expression... Alyssa Cecchetelli...

  13. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ... question about what should go into a cell, hover over the cell and instructions will pop up. When you...spreadsheet (Figure 2) and are described in Table 1. Some cells have drop-down options for you to choose from, while... into the appropriate full and partial sequence cells. *Note: If you believe you need to deposit a collection...Relevant Mutations Indicate the amino acid change as well as any functional consequences of that change. For...

  14. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    ...encode proteins. They play important roles in many cellular and regulatory processes, but much of their functionality...RNA (sgRNA) to create a double-strand break works well because very small deletions and insertions in the... should be able to devise an experiment that can tell you more about its function.  Resources and references...screening for functional long noncoding RNAs in human cells by Cas9 targeting of splice sites. Nat Biotechnol...non-coding RNAs with the CRISPR/Cas9 system in human cell lines. Nucleic Acids Research. 2014;43(3):e17. doi...

  15. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ...plethora of antibody options for your experiment (all well validated for your application, of course!) When...the isotype, and subclass affect my experiment?” Well, it depends on the application and target. If you... blot and probing a single target that expresses well, then probably not. In this case, take a look at...degree of affinity, then species choice could very well matter. Take rabbit versus mouse monoclonal antibodies...for Light and Electron Microscopy. In Methods in Cell Biology (pp. 283–310). Elsevier. https://doi.org...

  16. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    ...better than a nitrocellulose membrane. If you have already run your blot using nitrocellulose, you may decide...protein of interest in a complicated solution like cell lysate. But they can be a lot of work — and what...instructions for the membrane you are using. Dry nitrocellulose membranes are brittle, so proceed with caution...to solutions. It is also volatile and toxic (and smells bad!), so be sure to use it under a fume hood. ...

  17. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    ...since the lab was highly focused on protecting intellectual property, it worked away in isolation. It published...joint. Silly ideas (mining local salt deposits to sell at farmers markets), and serious ideas (how to stop...color-changing flowers was a great business idea as well, and they thought we could be taught. They invested...Biochemistry Department at the University of Manchester as well as the Biology Department at CSU. When not pursuing...enjoys spending time with her husband and two Jack Russell Terriers. More SynBio Reading Read more Addgene...

  18. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    ... in mammalian cells Hu et al. compared xCas9 activity with wt SpCas9 in mammalian cells, finding xCas9...ABE7.10, as well as improvements at NG and GAT PAMs. xCas9 3.7 Activity in Mammalian Cells Construct... percent editing efficiencies (edit frequency in cell population measured with NGS) are shown as percentages...

  19. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

    Type
    Blog Post
    ...overexpress YFG in a Drosophila cell line. Knock downs in Drosophila cell lines are extremely effective....days after setting up the genetic cross). You can tell when pupae are ready to hatch the next day because...person. Animals are diploid except for their germ cells (sets of genomic material are contributed by mother...various fly reagents on the Bloomington website as well. Additionally, they have useful information on topics...

  20. Academic vs. Industry Postdocs

    Type
    Blog Post
    ...doing a brief academic postdoc. Why did I switch? Well, my long-term goal was to enter industry since I...” that you’ve sold out. You’ve given up your intellectual freedom and integrity. (Not the case, as I’ve...s ability to manage others and work as a team as well as their scientific abilities. At AstraZeneca, scientists...who get postdocs and do not support or manage them well are simply no longer allowed to have postdocs. In...Scientist at AstraZeneca. She has a background in cell and developmental biology and is applying this towards...
Showing: 701 - 720 of 1033 results