We narrowed to 805 results for: Eng

1. The time and cost required to make a plasmid

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   Blog Post

   ...these rough estimates, it’s clear that community engagement with and use of repositories like Addgene provides...

2. Plasmids 101: The protein expression toolbox

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   Blog Post

   ...promoters Tetracycline-dependent promoters are engineered by placing tet response elements upstream of ...

3. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

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   Blog Post

   ...structural knowledge of the proteins. To overcome this challenge, the Uppalapati and Woolley lab developed a phage...

4. Reproducibility for Everyone: Lessons from an Open Science Collaboration

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   Blog Post

   ...working with communities, not for them - Another strength of this collaboration is that it is driven by ...

5. RNA Extraction Without A Kit

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   Blog Post

   ...until homogenization and cell lysis. This can be challenging depending on your laboratory situation or tissue...

6. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

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   Blog Post

   ...editors at Addgene. Levy et al., Nature Biomedical Engineering, 2020. PubMed PMID: 31937940. Detection of neural...

7. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

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   Blog Post

   ...Galvan et al., 2019). The hM4Di receptor is an engineered Gi protein-coupled receptor, one of the Designer...

8. RaPID Detection of RNA-protein Interactions

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   Blog Post

   ...bind an RNA motif of interest in living cells. Challenges with existing RNA-protein interaction methods...

9. Supporting Rare Disease Research with the 2020 BeHEARD Awards

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   Blog Post

   ... Empower and Accelerate Research Discoveries) Challenge provides grants for rare disease research. This...

10. Summer SciComm: Preprints

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    Blog Post

    ...and feedback giving authors the opportunity to strengthen the manuscript prior to submission. Preprints...

11. Human Germline Editing Using CRISPR

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    Blog Post

    ...heterozygous mutations, it will clearly be much more challenging for homozygous mutations. Moreover, introducing...

12. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

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    Blog Post

    ...new plasmids and/or cells or organisms that are engineered can be shared to simplify the comparison by others...

13. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

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    Blog Post

    ...~6.5 - 9 *Optimal sequential excitations wavelengths as determined by Rosado et al: see Table 2 for...

14. Developing Lab Management Software for Biology

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    Blog Post

    ...in on the fun too. Kris Shamloo is a Software Engineer at Addgene and is interested in writing software...

15. Antibodies 101: Stripping and Reprobing Western Blots

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    Blog Post

    ...time you strip a blot, you should not compare the strength of bands from different rounds of detection. Finally...

16. Viral Vectors 101: Chemogenetics

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    Blog Post

    ...Chemogenetics relies on genetically introducing engineered receptors that are only activated by otherwise...

17. Antibodies 101: Choosing the Right Isotype

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    Blog Post

    ...secondary antibody binding and therefore the signal strength (Fig. 1). Monoclonal primaries If you do decide...

18. Adenoviral Vector Production and Troubleshooting

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    Blog Post

    ...not integrate into the host’s genome. The main challenge with using AdV is that it triggers a strong immune...

19. Educational Planarian Tools for Teaching Developmental Biology Techniques

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    Blog Post

    ...experiments are either not accessible or too challenging for amateur scientists. Planarians, wormy creatures...

20. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

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    Blog Post

    ...T-to-G•C base changes (Figure 1), but it can be challenging to make these base changes, especially in primary...