We narrowed to 802 results for: Eng

1. Reproducibility for Everyone: Lessons from an Open Science Collaboration

   Type

   Blog Post

   ...working with communities, not for them - Another strength of this collaboration is that it is driven by ...

2. Supporting Rare Disease Research with the 2020 BeHEARD Awards

   Type

   Blog Post

   ... Empower and Accelerate Research Discoveries) Challenge provides grants for rare disease research. This...

3. RNA Extraction Without A Kit

   Type

   Blog Post

   ...until homogenization and cell lysis. This can be challenging depending on your laboratory situation or tissue...

4. Summer SciComm: Preprints

   Type

   Blog Post

   ...and feedback giving authors the opportunity to strengthen the manuscript prior to submission. Preprints...

5. Viral Vectors 101: An Introduction to AAV

   Type

   Blog Post

   ...and a self-complementary AAV (scAAV) vector. Full-length ssAAV vector genome of both polarities are rescued...

6. Transferable Skills Guide: Public Speaking

   Type

   Blog Post

   ...gives your entire audience a chance to comfortably engage with you. If you can give a seminar, lead a journal...

7. The time and cost required to make a plasmid

   Type

   Blog Post

   ...these rough estimates, it’s clear that community engagement with and use of repositories like Addgene provides...

8. Plasmids 101: The protein expression toolbox

   Type

   Blog Post

   ...promoters Tetracycline-dependent promoters are engineered by placing tet response elements upstream of ...

9. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

   Type

   Blog Post

   ...Galvan et al., 2019). The hM4Di receptor is an engineered Gi protein-coupled receptor, one of the Designer...

10. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type

    Blog Post

    ...editors at Addgene. Levy et al., Nature Biomedical Engineering, 2020. PubMed PMID: 31937940. Detection of neural...

11. Developing Lab Management Software for Biology

    Type

    Blog Post

    ...in on the fun too. Kris Shamloo is a Software Engineer at Addgene and is interested in writing software...

12. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

    Type

    Blog Post

    ...features that allow such “chemical rescue” in vivo. Engineering the human disease alleles into cells for testing...

13. Adenoviral Vector Production and Troubleshooting

    Type

    Blog Post

    ...not integrate into the host’s genome. The main challenge with using AdV is that it triggers a strong immune...

14. Educational Planarian Tools for Teaching Developmental Biology Techniques

    Type

    Blog Post

    ...experiments are either not accessible or too challenging for amateur scientists. Planarians, wormy creatures...

15. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type

    Blog Post

    ...outside of the reader’s field of view because of its length, and will be more difficult to read. The Thompson...

16. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

    Type

    Blog Post

    ...T-to-G•C base changes (Figure 1), but it can be challenging to make these base changes, especially in primary...

17. Viral Vectors 101: Chemogenetics

    Type

    Blog Post

    ...Chemogenetics relies on genetically introducing engineered receptors that are only activated by otherwise...

18. Antibodies 101: Choosing the Right Isotype

    Type

    Blog Post

    ...secondary antibody binding and therefore the signal strength (Fig. 1). Monoclonal primaries If you do decide...

19. Antibodies 101: Stripping and Reprobing Western Blots

    Type

    Blog Post

    ...time you strip a blot, you should not compare the strength of bands from different rounds of detection. Finally...

20. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type

    Blog Post

    ...DNA Extracts. Pooled libraries are a bit more challenging to QC by NGS as they are often quite different...