We narrowed to 809 results for: Mak

1. Plasmids 101: Methylation and Restriction Enzymes

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   Blog Post

   ...or for diagnostics, we suggest double checking to make sure your results will not be affected by methylation...

2. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

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   Blog Post

   ...machinery to process gRNAs. Hijacking tRNA processing to make gRNAs In a recently published paper, Yinong Yang...

3. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

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   Blog Post

   ... and devoted 100% of his energy and effort into making the startup self-sustainable. Within a year from...

4. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

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   ... away. As a result, variable gene expression can make data hard to interpret or can interfere with the...

5. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

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   ...summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you...

6. Human Germline Editing Using CRISPR

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   ...differences in editing between iPS cells and embryos make a case for conducting research in embryos should...

7. Choosing the B(right)est Fluorescent Protein: Photostability

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   ...issues with some of the experimental designs used to make these measurements and hope this information can...

8. Transferable Skills Guide: Problem Solving

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   ...other skills such as critical thinking, decision making, creativity, or data analysis. Conveniently, these...

9. Transferable Skills Guide: Cross-team Communication

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   ...We took steps towards solving this problem by 1) making sure we qualified these words when we discussed...

10. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

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    Blog Post

    ...bioelectrical signals affect pattern memory and decision-making in somatic cell networks. “Developmental bioelectricity...

11. Troubleshooting Your Plasmid Cloning Experiment

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    Blog Post

    ...Be mindful of the restriction enzymes you chose. Make sure that enzymes that you are using are not blocked...

12. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

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    Blog Post

    ...summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you...

13. Supporting Rare Disease Research with the 2020 BeHEARD Awards

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    Blog Post

    ... the main source of IL-36 cytokines in the skin making it important to study what is happening within ...

14. Giving gRNAs a Facelift - Synthetic and Beyond

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    Blog Post

    ...modifications What is more stable than RNA? DNA! One way to make your gRNA more efficient on a budget is to simply...

15. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

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    ... has revolutionized how we decode the genome by making it easy to create specific genetic perturbations...

16. New Tool for Lineage Tracing: The ClonTracer Library

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    Blog Post

    ...deposited the library with Addgene because she wanted to make it as easy as possible for researchers to use this...

17. Plasmids 101: Cre-lox

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    ...types, during certain stages of development, or by making the Cre inducible (such as with tamoxifen or doxycycline...

18. Working with Nuclear Receptors

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    ...with a one-stop shop for plasmids and vectors, and making them available to all researchers for a reasonable...

19. Viral Vectors 101: Virus Safety

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    Blog Post

    ...having an ‘off’ day – you are both more likely to make a mistake and more likely to want to cut a few corners...

20. Antibodies 101: Choosing the Right Isotype

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    Blog Post

    ...antibodies rather than generally reactive ones. And make sure to check for any reported cross-reactivity ...