We narrowed to 940 results for: Mpl;
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  TypeBlog Post..., in turn, the big picture of the project. For example, how can future regulations impact your work? What...sounds interesting, TAKE ACTION! Taking action: Examples of extracurricular activities The table below...
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Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools
TypeBlog Post...Thus, the bEFPs bind the UAS via the dCas9-gRNA complex which allows transcription from the promoter. ...should serve as both a powerful tool for generating complex regulatory systems and further the study of natural... - 
  
Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping
TypeBlog Post...binds to TIR1 of the SCF-TIR1 ubiquitin ligase complex, promoting its interaction with the degron sequence...used ChRs in that they cause a rapid and almost complete desensitization when exposed to continuous bright... - 
  
New and Upcoming Viral Vectors - June 2019
TypeBlog Post...size 20 µL samples of control AAV that scientists can use for serotype testing. These samples are available... - 
  
Virtual Science Conference Coming Up? Three Addgenies Share Their Experience
TypeBlog Post...other ways to make your session more engaging, for example, by using short animations and visuals, or asking...be a “place” for networking within the app. For example, the NeuroSymposium had virtual tables that you... - 
  
Trading Spaces - Visiting Scientist Edition
TypeBlog Post...from a month to a year. The researcher remains employed by their home institute, meaning the host university...and a priority list of experiments you hope to complete. Share this plan with your host prior to arrival... - 
  
Hot Plasmids - January 2023
TypeBlog Post...JA. Precise transcript targeting by CRISPR-Csm complexes. Nat Biotechnol. 2023 Jan 23. doi: 10.1038/s41587...antibodies allows you to use them in parallel to help amplify your signal - giving you the benefit of a polyclonal... - 
  
Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors
TypeBlog Post...breaks down the HMO of interest into its component simple sugars (including lactose), and a lac operon controlled...to determine the amount of HMO originally in the sample. The second sensor is a fusion protein consisting... - 
  
Predicting Adverse Reactions to Monoclonal Antibody Drugs
TypeBlog Post... binding affinity to major histocompatibility complexes (MHC class I and II). There are multiple algorithms...approach The in silico approach cannot replicate the complexity of antigen processing events that take place ... - 
  
Choosing Your Fluorescent Proteins for Multi-Color Imaging
TypeBlog Post... proteins should be separated by 50-60 nm. For example, CFP (ex 430 nm / em 474 nm) and YFP (ex 514 nm...protein of interest has a similar spectrum. For example, here's a screenshot from the Chroma Spectra Viewer... - 
  
Communicating Your Science With Help From ComSciCon
TypeBlog Post...an interview setting, this means persuading an employer that your past work has prepared you to contribute...audience and ask for their feedback before you complete your work. If you’re giving a public talk, ask... - 
  
Why and how to start studying chromatin (with Addgene's help)
TypeBlog Post...interact with each other. Chromatin regulation is complex and influences--directly or indirectly--many other...phosphoinositol-dependent binding of the SWI/SNF-like BAF complex to chromatin after T lymphocyte receptor signaling... - 
  
Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection
TypeBlog Post...plasmid can give them different phenotypes. For example, this plasmid encodes a green fluorescent protein...eukaryotic cells via a virus or viral vector. One example are bacteriophages that attach to bacterial membranes... - 
  
Viral Vectors 101: Types of viruses
TypeBlog Post...that use reverse transcriptase to synthesize complementary DNA copies of their genome upon infecting a ... These viruses have a cargo capacity of ~ 8 kb, ample room for most experimental needs, and can transduce... - 
  
Viral Vectors 101: Viral Applications
TypeBlog Post...shRNAs are short RNA sequences which target complementary mRNA sequence for degradation. Viral introduction...targeting an exon in your gene of interest, for example. Some customization is required, but many viral... - 
  
A Deep Dive into BioBuilder
TypeBlog Post... in March to exhibit projects at any stage of completion, from pitch to prototype. Last year’s BioBuilderClub... for a local school’s Science Department, or to simply tour the lab as a starting point into further STEM... - 
  
Luminescent Imaging with Nano-lanterns
TypeBlog Post... handy. This approach is especially suited to complement optogenetic tools, where light used for excitation...external light source. Therefore, especially thick samples may not be appropriate for use with Nano-lantern... - 
  
How Addgene Creates Content
TypeBlog Post... look at how we create this content, using the example of our Antibody Guide, which was published alongside...require updating or retiring. CRISPR/Cas9, for example, has gone through rapid change in the past few ... - 
  
OCaMP: A New Calcium Indicator for Neural Imaging
TypeBlog Post...jRGECO1a and jRCaMP1a are widely used as well. To complement these tools, we recently developed a new orange...cell types at once using existing indicators. For example, you might want to use OCaMP to monitor Ca2+ signals... - 
  
Fluorescent Proteins 101: Luciferases
TypeBlog Post...chemical reaction takes place. Figure 2: Simplified chemical reaction catalyzed by luciferase, releasing... proteins to be visualized simultaneously. Complementation systems for examining protein-protein interactions...