We narrowed to 910 results for: Nes

1. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

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   Blog Post

   ...dissociate from the ligase and bind to exposed lysines on neighboring proteins. Thus to capture protein...monitor protein-protein interactions Split-BioID combines a protein fragment complementation (PCA) technique...

2. An Integrin Antibody Toolkit from IPI

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   ...development, extended-open integrins enable tissue morphogenesis, organ development, and the shaping of the extracellular...the scientists and scientific process behind the scenes to the new antibodies and research coming out of...

3. New Optogenetic Tools for Cytoskeleton and Membrane Control

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   ...constructs for targeting Golgi, ER, and endosome membranes) (Figure 4). The authors used SuperPLD to manipulate...illumination, substantial PA accumulates in the plasma membranes, indicated by the PA probe colocalizing with SuperPLDx30...

4. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

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   ...a challenge. Enter Easi-CRISPR: a method that harnesses the power of ssDNA donor molecules for homology...mouse embryonic stem cells. After selecting for clones with the recombined product, you’d microinject ...

5. Building Global Connections with the International Mentorship Program USA-EUROPE

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   ...skills, positive disposition and motivation, open mindedness, and the ability to accept feedback and adapt...receive immense personal gratification as they witness their student’s progress and increases in self ...

6. When Fidelity Matters: A frank discussion about ligase fidelity

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   ...PMC1933048. 16. Cheng, Changming, et al. "Electrochemiluminescent assay for detection of extremely rare mutations... probe DNA-immobilized magnetic beads and chemiluminescence detection." Analytical Sciences 29.7 (2013...

7. Deep Dive: Statistical Tests (Comparisons)

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   ...Statistics – The basics Statistical analysis is what determines if there is statistical significance among data...There are many, many statistical tests beyond the ones detailed in this article. These are just some of...

8. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

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   .... Yu, J., et al., Induced pluripotent stem cell lines derived from human somatic cells. Science, 2007....duodenal homeobox gene 1 induces expression of insulin genes in liver and ameliorates streptozotocin-induced ...

9. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

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   ...of prime editing tools. We’ll summarize the major ones in Addgene’s repository later in the post. Advantages...). Efficient site-specific integration of large genes in mammalian cells via continuously evolved recombinases...

10. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

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    ... knowledge to inform on the qualities of what determines good sgRNA design. Incidentally, the first software...Cas-OFFinder will suffice. Cas-OFFinder provides a bare-bones data dump of ALL strings in a genome using any IUPAC...

11. Thanks to Addgene Depositors, We’re Now Sharing over 100,000 Reagents!

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    ...grade DNA!     This is just the first of many milestones Addgene is poised to reach in the near future...

12. 2022 Depositor Week

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    ...this is it!)  Tuesday: Stats roundup on Twitter Wednesday: 2022 Blue Flame Depositors Hall of Fame  Thursday...

13. Hot Plasmids Podcast Episode 2: New RFPs, AAVs, & More

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    ...Toolbox for quickly generating zebrafish transgenic lines to study neurodegenerative diseases. Listen to Our...

14. It’s CRISPR Week at Addgene!

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    ...focus on generating knockouts using CRISPR and Wednesday will highlight some Cas9 variants. Next, we’ll...

15. Addgene's 20th Anniversary Party

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    ...words of welcome! Executive Director Chonnettia Jones and Board Member Joanna Brownstein were also on ...

16. New FLIS Plasmid (April Fools 2022)

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    ...Light Interval System, or FLIS, incorporates a bioluminescence protein into the chitin that can be activated...

17. Lucky Thirteen: Guest Contributors of 2023

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    ...)Author: Jason PellettieriNew Acoustic Reporter Genes: Ultrasound Imaging of Gene ExpressionAuthors: Marjorie...

18. Overwhelmed? Take a Break With Our 5 Favorite Science Comics

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    ...) ourselves back into our regular post-holiday routines, why not take a little time to appreciate the ...

19. Congrats to the 2019 Deck The Lab Winners

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    ...from lab gloves. The tree was adorned with a bioluminescent tree topper, tube “lights,” and autoclave tape...

20. Enabling high school research at the Journal of Emerging Investigators

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    ...published their work on a protein required for cytokinesis in JEI. Developing an interested in biology, ...