We narrowed to 1,017 results for: PLE;

...should receive the clinical sample to test their phages. Because the sample is limited, only the labs that...targeting organisms closely related to the clinical sample. For treatment, it is best to identify a cocktail...

...Science Communication group started. It's as simple as getting people together at a bar. Chapter 10: Maintaining...well as a platform for scientists to be viewed as people (#ActualLivingScientists). We also discuss the ...

...website as a “Publication Citing this Plasmid” - this simple act directly associates you with a network of researchers...functionality can be found in the following video: Please remember that the accuracy and reliability of our... plasmid is missing from our collections pages, please shoot us an e-mail at [email protected]. We’d love...

...affected by devastating diseases that affect far fewer people and therefore fail to garner much international...the Institute of Biosciences and Bioresources in Naples, Italy. His group works on spinal muscular atrophy...data from primary peritoneal mesothelioma tumor samples that they have used to identify candidate genes...

...YFP) to the new and improved fruit colors (e.g., apple, papaya, and tomato) and the photoactivatable fluors...quickly. Researchers should keep in mind that a very simple sequential or double restriction enzyme digest ...on the corresponding plasmid pages (e.g. see Supplemental Documents here). If you’re not sure where your...

...observe what’s happening inside cells for at least a couple of decades. Green is the classic color, but fluorescent...signal-to-background ratio, thus, allowing higher resolution in samples containing substantial autofluorescence background...direction of biosensors capable of revealing, for example, how muscles work inside a living animal. For those...

...University of Akron, Ohio. Congratulations! There was plenty of geeky fun to be had at ASM Microbe as well as...science theme, the Artwalk on the exhibit floor had plenty of wonderfully nerdy art for sale, and photos of...presented at the conference, it looks like there are plenty more exciting plasmid tools on the horizon for ...

...kind of walking them through the steps of, for example, cutting open a heart to look at the inside. We...a lot of interactives and videos, as well, to supplement the hands-on [aspect of the labs],” says Caitlin...new technologies in education and ask ‘Can we implement that?’ [And think about] where we [eScience] are...

...Iowa in the Department of Pharmacology, I taught a couple of lectures in the undergraduate pharmacology course... editing sphere.” But things aren’t always that simple, especially when your spouse is also in science... stumbled upon eScience by luck, by chance, by complete happenstance. It was on Indeed or one of the job...

...helps improve your interpersonal relationships with people you’re collaborating with remotely. Depending on...as Slack. Share data and documents While having multiple scientists edit the same document may sound like...collaborators. Being able to present data from multiple aspects of a collaboration and immediately discuss...

...CRISPR Antimicrobials Day 7: Multiplexing and Cas9 variants: CRISPR 101: Multiplex Expression of gRNAs and ...Cpf1: A New Tool for CRISPR Genome Editing and Multiplex Genome Editing with CRISPR-Cpf1 Day 4: Edit validation...

...skills help when it comes to finding a job. Many people in science will work in industry or take on collaborative...and their interest in science more generally to people outside of academia. Here are my recommendations... they start asking you questions. Remember that people like a good story and science involves characters...

...jGCaMP7 sensors with different properties (for example, overall brightness or on/off kinetics) that makes...quantify targeting of particular cell types. For example, the mDlx enhancer restricts expression to GABAergic... particular catalog item is available for order please email [email protected] with the vector name and...

...incorporate into their own labs. This week we are pleased to announce the submission of the results of our...be examined. Increased yields with sucrose supplementation is serotype specific In our study we observed...preparations for, we wanted to be certain that this supplementation strategy would have no effect on the purity...

...ever like to write about a recent plasmid deposit please sign up here.   Listen this episode of Hot Plasmids...APOBEC3A-Cas9n-UGI and APOBEC3B-Cas9n-UGI base editing complexes are more efficient than the BE3 plasmid containing...exhibiting its versatile utility in quantifying multiple gene editing activities. Click here to find the...

...common lexicon for genome editing studies. These complementary goals are aimed at improving translation of ... loosely paraphrased in the tweet below. The complexity and challenges of homology directed repair (HDR...editing and low off-target editing rates across multiple systems. Read our introductory blog post, then...

...manipulated with mutations in the replicon. For example, the pMB1 ori maintains about 20 copies per cell... any questions? Need something clarified? If so please, post in the comments.  References Chatterjee A...Schultz PG. A versatile platform for single- and multiple-unnatural amino acid mutagenesis in Escherichia...

...an ITR during routine plasmid propagation, with complete ITR loss abolishing AAV packaging. To prevent ... like as long as it’s ~4.4 kb or smaller. Some examples include a reporter like GFP, some smaller CRISPR-Cas...also drive different levels of expression. For example, CAG is a strong promoter that drives very high...

...Balazs. “By combining those with [sera] we got from people that have been given the vaccine, we've been able...used live virus (Cele et al., 2021). “We weren't completely shocked [with these results] because we were ... AG, Iafrate AJ, Naranbhai V, Balazs AB (2021) Multiple SARS-CoV-2 variants escape neutralization by vaccine-induced...

...many different things in parallel.” Alternating Hemiplegia of Childhood Melodi McSweeney (right), a graduate...responsible for the disease known as Alternating Hemiplegia of Childhood (AHC). Patients affected by AHC ...from recurrent attacks of unilateral paralysis (hemiplegia) that are often painful and accompanied by migraines...