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We narrowed to 1,010 results for: PLE;

Showing: 701 - 720 of 1010 results

  1. In Living Color: The Skinny on In Vivo Imaging Tools

    Type
    Blog Post
    ...observe what’s happening inside cells for at least a couple of decades. Green is the classic color, but fluorescent...signal-to-background ratio, thus, allowing higher resolution in samples containing substantial autofluorescence background...direction of biosensors capable of revealing, for example, how muscles work inside a living animal. For those...

  2. Fostering communication in remote collaborations

    Type
    Blog Post
    ...helps improve your interpersonal relationships with people you’re collaborating with remotely. Depending on...as Slack. Share data and documents While having multiple scientists edit the same document may sound like...collaborators. Being able to present data from multiple aspects of a collaboration and immediately discuss...

  3. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    ...CRISPR Antimicrobials Day 7: Multiplexing and Cas9 variants: CRISPR 101: Multiplex Expression of gRNAs and ...Cpf1: A New Tool for CRISPR Genome Editing and Multiplex Genome Editing with CRISPR-Cpf1 Day 4: Edit validation...

  4. Oh, The Places You Can Go: Careers in Science Communication - Curriculum Development

    Type
    Blog Post
    ...kind of walking them through the steps of, for example, cutting open a heart to look at the inside. We...a lot of interactives and videos, as well, to supplement the hands-on [aspect of the labs],” says Caitlin...new technologies in education and ask ‘Can we implement that?’ [And think about] where we [eScience] are...

  5. Oh, The Places You Can Go: Careers in Science Communication - Product Development

    Type
    Blog Post
    ...Iowa in the Department of Pharmacology, I taught a couple of lectures in the undergraduate pharmacology course... editing sphere.” But things aren’t always that simple, especially when your spouse is also in science... stumbled upon eScience by luck, by chance, by complete happenstance. It was on Indeed or one of the job...

  6. Addgene at the ASM Microbe 2016 Conference

    Type
    Blog Post
    ...University of Akron, Ohio. Congratulations! There was plenty of geeky fun to be had at ASM Microbe as well as...science theme, the Artwalk on the exhibit floor had plenty of wonderfully nerdy art for sale, and photos of...presented at the conference, it looks like there are plenty more exciting plasmid tools on the horizon for ...

  7. 10 Ways to Share Your Science!

    Type
    Blog Post
    ...skills help when it comes to finding a job. Many people in science will work in industry or take on collaborative...and their interest in science more generally to people outside of academia. Here are my recommendations... they start asking you questions. Remember that people like a good story and science involves characters...

  8. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...4 of the Cong, et. al, 2013 Science paper. For example, if you want to use double nickase, you could express...selection marker to select transfected cells (for example, plasmid with a puromycin resistance cassette, ...individual genotyping (using Sanger sequencing, for example) on each colony in order to screen for positive...

  10. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    ...jGCaMP7 sensors with different properties (for example, overall brightness or on/off kinetics) that makes...quantify targeting of particular cell types. For example, the mDlx enhancer restricts expression to GABAergic... particular catalog item is available for order please email [email protected] with the vector name and...

  12. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

    Type
    Blog Post
    ...incorporate into their own labs. This week we are pleased to announce the submission of the results of our...be examined. Increased yields with sucrose supplementation is serotype specific In our study we observed...preparations for, we wanted to be certain that this supplementation strategy would have no effect on the purity...

  13. Plasmids 101: Origin of Replication

    Type
    Blog Post
    ...manipulated with mutations in the replicon. For example, the pMB1 ori maintains about 20 copies per cell... any questions? Need something clarified? If so please, post in the comments.  References Chatterjee A...Schultz PG. A versatile platform for single- and multiple-unnatural amino acid mutagenesis in Escherichia...

  14. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    ...an ITR during routine plasmid propagation, with complete ITR loss abolishing AAV packaging. To prevent ... like as long as it’s ~4.4 kb or smaller. Some examples include a reporter like GFP, some smaller CRISPR-Cas...also drive different levels of expression. For example, CAG is a strong promoter that drives very high...

  15. CRISPR Challenges: Standardization and Homology Directed Repair

    Type
    Blog Post
    ...common lexicon for genome editing studies. These complementary goals are aimed at improving translation of ... loosely paraphrased in the tweet below. The complexity and challenges of homology directed repair (HDR...editing and low off-target editing rates across multiple systems. Read our introductory blog post, then...

  16. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type
    Blog Post
    ...many different things in parallel.” Alternating Hemiplegia of Childhood Melodi McSweeney (right), a graduate...responsible for the disease known as Alternating Hemiplegia of Childhood (AHC). Patients affected by AHC ...from recurrent attacks of unilateral paralysis (hemiplegia) that are often painful and accompanied by migraines...

  17. Mentoring for Scientists: I Have a Mentor, What Now?

    Type
    Blog Post
    ...discussion. The Forced Choice Analysis is a fun example and you can find it in our Mentoring for Scientists...help the Mentee feel a sense of accountability to complete action items and make progress. To illustrate ...with regular accountability checks. Focus on implementation of your goals and respect the time your Mentor...

  18. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    ... SARS-CoV-2 interacts with human proteins. For example, we used HEK293T cells in our first study, but ...collaborative science, and making it accessible to all people, not just scientists! *If you are requesting plasmids...plasmids for coronavirus or COVID-19 research, please email Addgene at [email protected] with your order...

  20. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    ... It doesn’t have to be this way. We can never completely eliminate the risk that a minor variation in ...collective priority list. There has been progress—for example, a number of funders (e.g., the NIH) now look for...Press.  She trained originally in cell biology, completing her PhD at the MRC LMB in Cambridge UK and then...
Showing: 701 - 720 of 1010 results