We narrowed to 1,014 results for: REN;
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TypeBlog Post...validated in. If a plasmid's Addgene ID # has been referenced in other publications, you'll find a link to ...Additional features Once you've clicked on the "# References" link under the "Resource Information" heading...Addgene’s Plasmids 101 eBook Protocols and Plasmid References @Addgene ...
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Designer PUF Proteins for Any RNA Target
TypeBlog Post...splicing, imaging, and more. Abil says she is currently working on a version that she hopes will change...PUF proteins lose their solubility. Her lab is currently investigating the issue and hopes to have a better...happy to share updated versions of the kit.” Reference Abil Z, Denard CA, Zhao H. Modular assembly of... -
Developing Transferable Skills During Science Training
TypeBlog Post...colleagues you help will be future advocates and references. Collaborate with another lab, in another field... a committee and do something big like plan a conference Recruit many supporters and mentors that will...skills too. Teach classes Go to workshops and conferences on interviewing, resume writing, and networking... -
Let There Be LITE Plasmids
TypeBlog Post...the researchers applied the LITE system to 28 different genes to establish it as “a novel mode of optogenetic...activators are easy to expand or combine with different effectors. I’d encourage people to get creative...readers, how will you get creative with LITE? Reference: Silvana Konermann et al. "Optical control of ... -
Stepping into a Virtual Internship: My Experience at Addgene
TypeBlog Post...internship would be a perfect way of exploring my different interests but also a way of challenging what I...would look like. While working remotely was a different experience, It was helpful to have Addgenies reassure...spoke with amazing people that were a part of the different departments of the company. I asked questions ... -
A new platform for fast functional characterization of potentially cancerous mutations
TypeBlog Post... a given mutation will have a different phenotypic effect in a different system. These plasmids can also...well-studied candidates. The possibilities are vast. References Ng, Patrick Kwok-Shing, et al. "Systematic functional... -
Six Spooky Science Stories and Halloween at Addgene
TypeBlog Post...nervous system: infected grasshoppers expressed different proteins in their brains compared to uninfected...and geotactic behavior (Biron et al., 2005). References Biron, D. G., et al. "Behavioural manipulation...Isopoda)." Copeia 1983.3 (1983): 813-816. Gilbert, Lawrence I. "Halloween genes encode P450 enzymes that mediate... -
Searchable and Sortable gRNAs for Your Next CRISPR Experiment
TypeBlog Post... gRNA table You can access the datatable here. Current table columns are target gene, species, sequence...ID, and depositor. The CRISPR applications we currently have data for are: cut, activate, interfere, visualize...jointly by Brook Pyhtila and Nicole Waxmonsky. References McDade J, Waxmonsky NC. Emerging CRISPR Technologies... -
IDP and your PI: A Roadmap for Career Planning and Personal Development
TypeBlog Post...section! References 1. Yearly Planning Meetings: Individualized Development Plans Aren't Just More Paperwork...you can identify suitable careers based on your current interests and skillset. With this information in... -
Easing the Protein Purification Process with pCri
TypeBlog Post... might not fold well, you should screen a few different vectors to see which gives you the most functional...two sets, each of which includes plasmids with different varieties of tags, both N- and C-terminal. Each...individually or as a complete kit from Addgene. References 1. Goulas, Theodoros, et al. "The pCri system... -
Science as Art
TypeBlog Post...LA, where it is currently on display. Many universities are now following the trend and encouraging... biology and I believe it could have made the difference in some areas of conservation.” Appreciating ... -
CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]
TypeBlog Post... by Daniel Bauer and Matthew Canver of Boston Children’s Hospital and Harvard Medical School. Addgene ...hematologist and principal investigator at Boston Children’s Hospital and Harvard Medical School. His lab ...Bioengineering from the University of Pennsylvania. References: 1. Lee HJ, Kim E, Kim JS. Targeted chromosomal... -
Inside Addgene's 10 Year Anniversary Gala
TypeBlog Post...Addgene grow into the successful, internationally renowned plasmid repository that it is today. Although ...faces of some of our depositing scientists who weren’t able to join us in person. Joanne Kamens, Melina...entire night was when Melina and Ken called their parents up to the podium to be recognized. John and Stephanie... -
Celebrating Accomplishments in the Lab
TypeBlog Post...lots of interesting things from children, their classmates, and parents”. He also cooks and cleans with...academics because it offers more flexibility and you aren’t locked into a schedule.” George Church comments... -
Three Tips to Organize Your Lab Notebooks in the New Year
TypeBlog Post...post-its to make finding your most used protocols & references easy to find It’s happened to all of us. It’s...consider using color coded binders and tabs (for different experiments or projects) to make it easier to ...small by identifying the protocols, guides, or references that your labmates ask to borrow most frequently... -
Science Guides
TypeGuide...Pooled libraries are single preparations of many different plasmids. Plasmids within a given library have...the same backbone, but they express or target different genes. Some libraries cover the majority of the...Find educational guides to learn more about the different types of viruses that are commonly used in research... -
Supporting Rare Disease Research with BeHeard 2017
TypeBlog Post...in diabetes and lymphoma research or the many different ways you can improve your heart health. These .... Like other forms of mesothelioma, there are currently no targeted treatments for peritoneal mesothelioma...rare disease research funding opportunities. References 1. Jones, C., et al. "PP09. 1–2352: Systematic... -
2023 and 2024: Reflections and Looking Ahead
TypeBlog Post...With renewed energy and optimism that comes at the start of every new year, it is a wonderful opportunity... from 1,106 labs in 504 organizations from 37 different countries, into our repository, while expanding... We will pursue opportunities to build on our strengths that have successfully served the scientific community... -
New Norepinephrine Indicators: nLightG and nLightR
TypeBlog Post...assistance with this blog post. References and resources References Feng, J., Zhang, C., Lischinsky, ... nLight nLight sensors are based on alpha-1a adrenergic receptor; nLightG contains circularly permuted... -
SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture
TypeBlog Post... to use the same peptide tag for all sorts of different protein systems and they shouldn’t get in the ...marker expression level. Labs have been trying different approaches to improve CTC capture, but I hope ...Addgene. I am a big fan; thanks for the T-shirt! References: SpyLigase peptide-peptide ligation polymerizes...