We narrowed to 761 results for: Rest

1. Plasmids 101: Stringent Regulation of Replication

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   Blog Post

   ...levels low even if plasmid copy numbers are high, restraining further replication.   Figure 3: Comparison...

2. Hot Plasmids Spring 2024

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   Blog Post

   ...extraneous genetic material to reconstitute the gene of interest, reducing the immunogenicity typically associated...

3. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

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   ... Massachusetts General Hospital with research interests in the fields of gene editing and stem cells. ...

4. Transferable Skills Guide: Creativity

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   Blog Post

   ...exercise: Consider the issue as if there were no restraints on your problem-solving resources. What would...

5. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

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   Blog Post

   ...they naturally find people with which they have interests and experiences in common. We often refer to this...

6. Neuronal labeling with Spaghetti Monster

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   Blog Post

   ...antigenic peptide sequences, attached to a protein of interest (POI), that facilitate immunohistochemistry (IHC...

7. Hot Plasmids - November 2023

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   Blog Post

   ...Credit: Zhuhao Wu, Weill Cornell Medicine.  Interested in this antibody, but wish it were from a different...

8. Changing Labor Laws Bring Increased Postdoc Wages

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   Blog Post

   ...Executive Director of Future of Research. He is interested in advocating for and with junior scientists,...

9. Healthcare Consulting: A Door to the Business of Life Sciences

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   Blog Post

   ...works as a healthcare consultant in Boston. He is interested in the business of life sciences and it's impact...

10. CRISPR Activation: A Practical Guide

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    Blog Post

    ... you can achieve using a CRISPRa. Your gene of interest might already be hitting an upper bound of activation...

11. Tips for CRISPR Gene Editing in Mice

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    Blog Post

    ... delete the entire gene? Perhaps you are more interested in the c-terminus and would like to target that...

12. The Challenges of Cell Culture

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    Blog Post

    ... there would be no viable viruses to study. Interestingly, due to certain high profile problems surrounding...

13. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

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    Blog Post

    ...lentiviral transfer plasmid encodes your insert of interest, flanked by long terminal repeat (LTR) sequences...

14. An Integrin Antibody Toolkit from IPI

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    Blog Post

    ...preferences of the αV subunit and its β partners. Of interest, we demonstrated that anti-integrin αVβ5.9 “essentially...

15. Transferable Skills Guide: Time Management

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    Blog Post

    ...Gilmore directly to see if he knows anyone who’s interested in meeting Send out as many emails as possible...

16. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

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    Blog Post

    ..., the partial genomes complement each other to restore the full-expression cassette. AAV’s natural ability...

17. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

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    Blog Post

    ...reads that we studied by deep sequencing, but interestingly (and to our frustration) in many cases we only...

18. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

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    Blog Post

    ...viral protein production, so researchers are also interested in precisely excising the HIV-1 genome from infected...

19. How to Negotiate a Successful Lab Start Up Budget

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    Blog Post

    ...This is actually why a department/institute is interested in you. Your science will define your success...

20. Hot Plasmids: Spring 2025

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    Blog Post

    ...promoter, tag, polyA site) are flanked by unique restriction sites so you can swap sequences effortlessly....