We narrowed to 939 results for: TIM
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TypeBlog Post...Class I TEs are considered replicative since every time they jump they make a copy of themselves. This increases...active in mammalian cells compared to the codon-optimized wild-type piggyBac transposase. piggyBac plasmids...
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A History of Genome Engineering in Popular Culture
TypeBlog Post...and vice versa? We draw a parallel between the timelines of the scientific advancements and the noteworthy...amino acid recognition sites had to be designed each time we wanted to target new genomic sites. The discovery... -
Quick Guide to All Things Lentivirus
TypeBlog Post...concentrate your lentiviral particles to get them to the optimum concentration to infect your cells of interest....References 1. Miller, A. DUSTY, and C. A. R. O. L. Buttimore. "Redesign of retrovirus packaging cell lines ... -
Antibodies 101: Designing Your First Flow Panel
TypeBlog Post...are the most efficient (i.e., happen most of the time). Figure 1 shows a graph from the BD® Spectrum Viewer...show efficient absorption at 650 nm, APC-Cy7's optimal excitation happens at higher wavelengths of about... -
Special Delivery: Fluorophore Targeting for FRET Studies
TypeBlog Post...This is not an extensive list Acceptor labeling: Sometimes it's okay to be wrong In contrast to FRET donors...PubMed PMID: 16908183. 10. Saraheimo, Satu, et al. "Time-Resolved FRET-Based Approach for Antibody Detection–A... -
New Western Blot Protocol Video!
TypeBlog Post... a Western blot can be a bit daunting for first-timers. That's why we gave our written protocol a video... -
Plasmids 101 eBook 4th Edition
TypeBlog Post...new sections, including our popular "Dimers and Multimers" post. It's also been re-organized and updated... -
Introducing...Addgene Quick Tips!
TypeBlog Post...techniques — but perhaps are a little limited on time — check out Addgene’s new Quick Tip series on YouTube... -
Viral Vectors 101 eBook 2nd Edition
TypeBlog Post...from producing your own vectors in the lab to optimizing AAVs in your experiment. Our second edition... -
#DeckTheLab to Win a Tasty Winter Treat This Holiday Season
TypeBlog Post...how you’re decorating your lab for the upcoming wintertime festivities! Help us spread some cheer by snapping... -
2022 Depositor Week
TypeBlog Post...welcome to Depositor Week 2022! It’s that special time of year where we Addgenies use our social media ... -
'Tis the Season to #DeckTheLab
TypeBlog Post...how you’re decorating your lab for all upcoming wintertime festivities: Hanukkah, Christmas, ugly sweaters... -
Meet Aavery, Blugene's New Virus Friend!
TypeBlog Post...person, we selected the finalist based on the first time of submission.) We love the way the names... -
Addgene's 20th Anniversary Party
TypeBlog Post...welcoming committee. However, the majority of the time was spent with Addgenies meeting each other's friends... -
It’s CRISPR Week at Addgene!
TypeBlog Post...the repository that have been shared over 140,000 times to over 75 countries. That’s a lot of CRISPR! As... -
Addgene is Stopping Use of X (formerly Twitter)
TypeBlog Post...conflict with our values. For this reason, our timeline has accelerated. We significantly reduced posting... -
Trends in CRISPR and SynBio Technologies
TypeBlog Post...Zinc Finger, TALE, CRISPR, and SynBio plasmids over time. You can see these data in the slide share. ... -
Participate in the Pets of Addgene Scavenger Hunt
TypeBlog Post... HQ and for some of us, that means spending more time with our pets. These new “co-workers” have made ... -
We’re Going Viral This Week!
TypeBlog Post... titer, serotype, injection site, and incubation time. Learn more about the Data Hub in this blog post... -
2021 Deck the Lab Winners!
TypeBlog Post...Ahem, ahem, drum roll please! It's time to announce the winners of our 2021 Deck the Lab Contest! Though...