We narrowed to 1,042 results for: URE

...Addgene is ending 2024 with big plans for our future. And while we’ve been excited to share them with...now-common business practices, like accepting e-signatures and designing a website users could easily navigate...grow accessibility alongside our repository.  “I’m sure you hear it all the time, but Addgene is a totally...institutions with standardized agreements and electronic signatures to dramatically speed up MTA approvals. In 2013...

...before me had been assigned a structure paper with two single-panel figures, one of which was a cartoon ...molecular biology class. A Cell paper. With six figures. Averaging ten panels each. (For this story, it...notebook, opened it, found my outline, read over Figure 4, took a deep breath, and, re-oriented, continued...

...plasmid, succumb to the toxicity of CcdB.   Figure 1: Image from Bernard, P., et al. showing the pKIL...still express ccdB and therefore would die. This procedure dramatically reduces the number of clones that...contain the desired insert should not grow.   Figure 2: Image from http://teachline.ls.huji.ac.il/72682...all of the players becomes ever more important to ensure that you choose the most appropriate method for...

...CARM1 methyltransferase. This attendant signaling feature afforded by coactivator complexes suggests that...the NURSA website, the new version of which will feature a simplified user interface, expanded numbers of...associated with identifying plasmids in the research literature and acquiring them in a timely manner. Addgene...accordingly, the new version of the NURSA website will feature direct links to listings of available plasmids ...

..., 2013). Two important features of this early system are its regulatable nature and its ability to multiplex...occurred. This powerful negative selection system ensures high editing efficiency; the only non-edited cells... pTargetF and pCas plasmids can be subsequently cured from the bacteria. Although developed in E. coli...Resources on the Addgene Blog Check out our CRISPR Featured Topic Page Learn more about Cpf1 Find out about...

...those which you’ve already fluorescently labeled. Figure 1: Using Split-BioID to Identify Protein Protein...that give the strongest biotinylation signal, as measured via Western blot, and that result in proper localization...these two bait proteins stop interacting. This feature is particularly useful for in-depth characterization... of spatiotemporally defined protein complexes. Nature communications. PubMed PMID: 28585547. PubMed Central...

...engineered CRISPR-Cas9 complex. Nature 517:583–588 . https://doi.org/10.1038/nature14136 Perez-Pinera P, Kocak ...acetyltransferase domain capable of rearranging chromatin structure. This method has shown higher activation levels...C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H, Nureki O, Zhang F (2014) Genome-scale transcriptional ...

...an anti-CRISPR protein. Nature 546:436–439 . https://doi.org/10.1038/nature22377 Harrington LB, Doxzen ... species of bacteria.  A note on anti-CRISPR nomenclature Acr family proteins are named for the type of... DNA cleavage (Harrington et al., 2017). Figure 1: Anti-CRISPR proteins (Acr's) can block CRISPR...

...gene delivery, but it has a drawback: how do you ensure your gene goes where you want it to? Knowing that...transcriptionally active in retinal cell types in mice. Figure 1: The four strategies used to design synthetic...AAVs would express Cre-mCherry and GFP.   Figure 2: Synthetic promoters can be paired for targeted...promoter AAVs in mice, non-human primates and humans. Nature Neuroscience, 22, 1345-1356. https://doi.org/10.1038...

...inheritance: 1. Genotype your parental mice In order to ensure that your genetic modification will be passed along...genotype all of your F1 mice, as well as progeny from future generations. 4. Continue breeding your colony You...they are likely to stay on separate chromosomes in future generations as well, a concept known as linkage...expression of Cre, loxP, and your gene of interest. To ensure that the effects you see in your experiment are...

...et al. "cGAL, a temperature-robust GAL4–UAS system for Caenorhabditis elegans." Nature methods 14.2 (2017... detailed information about C. elegans 3’UTRs structures, alternative polyadenylation for all protein ... novel findings. These results are normally one figure and can be negative and/or even replicated studies...

...infections. She is particularly passionate about HIV cure research and science communication.     References...to the central and peripheral nervous systems." Nature Neuroscience 20.8 (2017): 1172-1179. PubMed PMID...affinity-purified receptor-targeted viral vectors." Nature communications 6 (2015): ncomms7246. PubMed PMID... the Addgene Blog Check out our Viral Vectors Featured Topic Page Learn about CRISPR-AAV vectors Learn...

...the impacts of neural circuits on behavior. Figure 1: An overview of the chemogenetics system. ...characterize the compound, or compounds, you choose to ensure reliable results (Goutaudier et al., 2019). Using...Yang X, Stone O, Roth BL, Jin J (2015) The First Structure–Activity Relationship Studies for Designer Receptors...designed Gi-coupled receptor in transgenic mice. Nature Biotechnology 17:165–169 . https://doi.org/10.1038...

...efficiently and accurately in a “single pot.’   Figure 1: Single-Pot Assembly: a) Graphic depiction of... sensor modules targeting a specific chromatin feature (e.g. a methylated histone such as H3K27me3), a...neuron-astrocyte, and cancer-stromal cell pairs.   Figure 2: Schematic of the interaction between the sender...quantification of this depletion using imaging-based measurements (Ashley et al., 2021).   Schematic showing...

...animal’s skull using dental cement (a similar procedure as implanting a guide cannula). A fiber optic ...optogenetics but may become the standard in the future. Now that your animals are all wired up and expressing...long-term optogenetic manipulation of neural circuits. Nature protocols. 2012;7(1):12-23. doi: 10.1038/nprot.2011.413...Neuroscience research. 2011;70(1):124-7. doi: 10.1016/j.neures.2011.01.007. PubMed PMID: 21238511. 6. Jeong, ...

...graduate school who were at AstraZeneca and could assure me that they were having a positive experience,... years. This time limit can feel like a lot of pressure. However, it means that your manager (and you)... choose. If publishing is important to you, make sure that your project will not involve proprietary compounds...reviewed by an internal committee before submission to ensure that it does not divulge any propriety information...

... explain the thinking behind the tool, its key features, how it works, and how to use it. Background on...frequency determination (CFD score) is used to measure specificity of a guide to the target. The CFD score...Optimal mutation in-depth One the most sophisticated features of CrispyCrunch is how it mutates guide sequences...more detailed information on using CrispyCrunch, be sure to check out the How To Guide. Conclusion CrispyCrunch...

...been used to track protein localization, cell structure, intracellular trafficking, and protein turnover...plasmids to fluorescently tag markers of cellular structures in mammalian cells. These plasmids use fluorescent...reversibly degraded after the addition of auxin to the culture medium. Any number of regulatory sequences, including...“A Comprehensive Toolbox for Genome Editing in Cultured Drosophila melanogaster Cells.” G3 (Bethesda) ...

... 25°C. At low temperatures like 18°C, flies take longer to develop. At high temperatures like 30°C, flies...drift away with each passing generation.  Another feature of balancer chromosomes is that they produce dominant... during meiosis. This can sometimes be a handy feature when setting up genetic crosses. More importantly...

...the same time, this project gives Snapgene great exposure to the community of biological scientists who ...institutions personally on conference calls and make sure to maintain these relationships by keeping in touch...an internal to an external connection is to make sure you keep open lines of communication with colleagues...interesting events, articles and newsletters I make sure to share them with a short note - this creates a...