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We narrowed to 1,035 results for: URE

Showing: 701 - 720 of 1035 results

  1. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    ...an anti-CRISPR protein. Nature 546:436–439 . https://doi.org/10.1038/nature22377 Harrington LB, Doxzen ... species of bacteria.  A note on anti-CRISPR nomenclature Acr family proteins are named for the type of... DNA cleavage (Harrington et al., 2017). Figure 1: Anti-CRISPR proteins (Acr's) can block CRISPR...

  2. Synthetic promoter AAVs for cell-type specific expression in retinal cells

    Type
    Blog Post
    ...gene delivery, but it has a drawback: how do you ensure your gene goes where you want it to? Knowing that...transcriptionally active in retinal cell types in mice. Figure 1: The four strategies used to design synthetic...AAVs would express Cre-mCherry and GFP.   Figure 2: Synthetic promoters can be paired for targeted...promoter AAVs in mice, non-human primates and humans. Nature Neuroscience, 22, 1345-1356. https://doi.org/10.1038...

  3. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

    Type
    Blog Post
    ...those which you’ve already fluorescently labeled. Figure 1: Using Split-BioID to Identify Protein Protein...that give the strongest biotinylation signal, as measured via Western blot, and that result in proper localization...these two bait proteins stop interacting. This feature is particularly useful for in-depth characterization... of spatiotemporally defined protein complexes. Nature communications. PubMed PMID: 28585547. PubMed Central...

  4. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ...infections. She is particularly passionate about HIV cure research and science communication.     References...to the central and peripheral nervous systems." Nature Neuroscience 20.8 (2017): 1172-1179. PubMed PMID...affinity-purified receptor-targeted viral vectors." Nature communications 6 (2015): ncomms7246. PubMed PMID... the Addgene Blog Check out our Viral Vectors Featured Topic Page Learn about CRISPR-AAV vectors Learn...

  5. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

    Type
    Blog Post
    ...engineered CRISPR-Cas9 complex. Nature 517:583–588 . https://doi.org/10.1038/nature14136 Perez-Pinera P, Kocak ...acetyltransferase domain capable of rearranging chromatin structure. This method has shown higher activation levels...C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H, Nureki O, Zhang F (2014) Genome-scale transcriptional ...

  6. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ...the impacts of neural circuits on behavior. Figure 1: An overview of the chemogenetics system. ...characterize the compound, or compounds, you choose to ensure reliable results (Goutaudier et al., 2019). Using...Yang X, Stone O, Roth BL, Jin J (2015) The First Structure–Activity Relationship Studies for Designer Receptors...designed Gi-coupled receptor in transgenic mice. Nature Biotechnology 17:165–169 . https://doi.org/10.1038...

  7. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    ...animal’s skull using dental cement (a similar procedure as implanting a guide cannula). A fiber optic ...optogenetics but may become the standard in the future. Now that your animals are all wired up and expressing...long-term optogenetic manipulation of neural circuits. Nature protocols. 2012;7(1):12-23. doi: 10.1038/nprot.2011.413...Neuroscience research. 2011;70(1):124-7. doi: 10.1016/j.neures.2011.01.007. PubMed PMID: 21238511. 6. Jeong, ...

  8. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    ...been used to track protein localization, cell structure, intracellular trafficking, and protein turnover...plasmids to fluorescently tag markers of cellular structures in mammalian cells. These plasmids use fluorescent...reversibly degraded after the addition of auxin to the culture medium. Any number of regulatory sequences, including...“A Comprehensive Toolbox for Genome Editing in Cultured Drosophila melanogaster Cells.” G3 (Bethesda) ...

  9. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

    Type
    Blog Post
    ... 25°C. At low temperatures like 18°C, flies take longer to develop. At high temperatures like 30°C, flies...drift away with each passing generation.  Another feature of balancer chromosomes is that they produce dominant... during meiosis. This can sometimes be a handy feature when setting up genetic crosses. More importantly...

  10. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

    Type
    Blog Post
    ... explain the thinking behind the tool, its key features, how it works, and how to use it. Background on...frequency determination (CFD score) is used to measure specificity of a guide to the target. The CFD score...Optimal mutation in-depth One the most sophisticated features of CrispyCrunch is how it mutates guide sequences...more detailed information on using CrispyCrunch, be sure to check out the How To Guide. Conclusion CrispyCrunch...

  11. Academic vs. Industry Postdocs

    Type
    Blog Post
    ...graduate school who were at AstraZeneca and could assure me that they were having a positive experience,... years. This time limit can feel like a lot of pressure. However, it means that your manager (and you)... choose. If publishing is important to you, make sure that your project will not involve proprietary compounds...reviewed by an internal committee before submission to ensure that it does not divulge any propriety information...

  12. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    ... new experimental species. Discovering ways to culture, propagate, transform, store, and share all of ... "for development of tools, approaches, and infrastructure necessary for direct tests of cause and effect...was excited to be invited to attend. My job is to ensure that, in general, these labs all include materials...from Old Faithful in 60 °C soils. Dendrobatidae (Lauren O’Connell)  Poison frogs are great for studying...

  13. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    ...the same time, this project gives Snapgene great exposure to the community of biological scientists who ...institutions personally on conference calls and make sure to maintain these relationships by keeping in touch...an internal to an external connection is to make sure you keep open lines of communication with colleagues...interesting events, articles and newsletters I make sure to share them with a short note - this creates a...

  14. Career Insights: Technical Support Specialist

    Type
    Blog Post
    ...stages (i.e. postdoc level) and can include the pressure to publish in high-impact journals, and the requirement...with a custom-made reagent to manipulate cells (manufactured by one of the larger biotech companies), and...company, less exposed to the fast dynamics and pressure which are often found in sales or lab-based roles...name a few). Depending on the particular company structure you may be able to move into one of these departments...

  15. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...sent to the AbuGoot lab for testing, they weren’t sure what they would find… but it was safe to say the...dual activities: processing pre-CRISPR RNA into mature CRISPR RNA and cleaving RNA at positions defined.... But they weren’t done engineering quite yet – surely, they thought, the integration rate could be improved...with the single-protein CRISPR effector Cas7-11. Nature. 2021 Sep;597(7878):720-725. doi: 10.1038/s41586...

  16. March for Science

    Type
    Blog Post
    ...Makes Better Science Nature: What Trump’s new travel ban means for science Nature: Meet the scientists...job of having evidence”, scientists want to make sure having evidence is the job of everyone in government...all you need to do is select your state. State Legislatures: To find the website to contact representatives...

  17. Five Popular Model Organisms

    Type
    Blog Post
    ... than studies in other model organisms or cell culture. For example, Scott Lowe’s lab utilized mice to...double-stranded RNA in Caenorhabditis elegans." nature391.6669 (1998): 806. PubMed PMID: 9486653. Hesselson... and its relationship to the human genome." Nature496.7446 (2013): 498. PubMed PMID: 23594743. PubMed ...organism for studying somatic mutations and cancer." Nature Education 1.1 (2008): 183. Zuber, Johannes, et ...

  18. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    ...painstakingly cloned into the perfect empty backbone, made sure to add the right tags to your gene, and may have...provide enough background for you to distinguish the features of any common lab strain and determine whether... DNA, and more. Many mutations that make these features possible are present in most commercial strains...of protein expression in E. coli and the common features found in those bacteria. Check our our blog post...

  19. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...experimental results and 2) perform targeted off-target measurements at those sites to get an idea of how high the...effects, you still need to do your due diligence to ensure they are indeed a non-issue. Above we mentioned...these outcomes. Rigorous analysis is essential to ensure off-target events are below a given threshold for...your risk level is, how to lower it, and how to ensure off-targets don’t mess with your results when they...

  20. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ... either category, but those are a subject for a future post! How are repressible promoters regulated...commonly used in yeast. In the first 24 hours of culture, when glucose is abundant and ethanol concentrations...during the late ethanol consumption phase of yeast culture. Repressible Binary Systems GAL4/UAS In Drosophila...the system, researchers have used split GAL4 architecture or combined the system with Flp/FRT, as reviewed...
Showing: 701 - 720 of 1035 results