...painstakingly cloned into the perfect empty backbone, made sure to add the right tags to your gene, and may have...provide enough background for you to distinguish the features of any common lab strain and determine whether... DNA, and more. Many mutations that make these features possible are present in most commercial strains...of protein expression in E. coli and the common features found in those bacteria. Check our our blog post...

... either category, but those are a subject for a future post! How are repressible promoters regulated...commonly used in yeast. In the first 24 hours of culture, when glucose is abundant and ethanol concentrations...during the late ethanol consumption phase of yeast culture. Repressible Binary Systems GAL4/UAS In Drosophila...the system, researchers have used split GAL4 architecture or combined the system with Flp/FRT, as reviewed...

...experimental results and 2) perform targeted off-target measurements at those sites to get an idea of how high the...effects, you still need to do your due diligence to ensure they are indeed a non-issue. Above we mentioned...these outcomes. Rigorous analysis is essential to ensure off-target events are below a given threshold for...your risk level is, how to lower it, and how to ensure off-targets don’t mess with your results when they...

...contest is over, we will host other contests in the future. Subscribe to the blog to stay in the know!   ... hunt works works: You will be trying to find pictures of Addgene pets with Addgene-branded clothing ...pet gives you a clue to help you find the next picture. If you find the next correct photo, you will see...

...Addgene gave to me: a method to capture promoter-enhancer 3D chromatin structures using CRISPR. On the eleventh... you’ve been enjoying our CRISPR blog series. Be sure to subscribe to the CRISPR blog posts so you don...

...flick the tube several times. This will help ensure ensure equal distribution. Lab tip #3: Barcode everything... the latest episode.  Have some suggestions for future tips?  Send your ideas to [email protected].  Additional...

... or hydroelectric in nature. The operation of -80C and -20C freezer temperatures, biosafety cabinet ventilation... lighting/HVAC requires a significant energy expenditure here at Addgene. These are critical systems that...

...key step to any experiment and can help prevent future headaches. To help you plan PEI transfections for...simple to make adjustments for the type of tissue culture dish and number of dishes used. Here’s to making... pHelper: 2 RepCap Select what type of tissue culture dish you will be performing the transfection in...

...What's a lab group in industry? The collaborative nature of working in industry and how delegating can empower...success. The importance of thinking about the big picture and not just the next experiment. Chapter 6: Advice... the treatment of various diseases, from heart failure to Alzheimer’s.  You can follow her on Twitter ...

...29:54 - 31:25 : Nature's defenses against gene drives. 31:26 - 33:08 : Plans for future work at the Wyss...advisory board where he will help shape Addgene's future. Listen to this episode for insights into the process...

...for years – searching our photo archives I found pictures back through 2009, though I suspect that our costume...months in advance. Our teams comb the news (pop culture events of the past year obviously make for good...takes home the ultimate prize – a glorious trophy. Sure it’s plastic and you have to share 1 trophy among...

...what the founders hope to see in the repository’s future. If you’ve ever been curious about what it takes...success Chapter 6 - Developing Addgene’s Brand and culture Chapter 7 - Building Addgene as a global organization...Tufts Launchpad, and more Chapter 10 - Addgene's Future, the research team, and the importance of sharing...

...and look forward to hearing from her in the future. Future awardees We will be opening the Addgene/Seeding...them the most. Check back here to learn more about future awardees and their inspiring research. Additional...

...perform in vivo screens in other cell types. A key feature of this system is a lentiviral gRNA expression ...the statistical power of in vivo screens. Figure 1: Using FITS to perform in vivo CRISPR screens...clonal dynamics in lymph nodes (LN) and tumor cells. Figure used with permission from Milling and Markson et...

...and proteins associated with pathogenesis. In his future work. Dr. Bengyella would like to use the expertise...to do excellent work in the biological sciences. Future awardees Now that this first round of awards has...enable scientists in developing regions in the near future. Additional Resources Apply to Recieve Equipment...

...also be expanded to include even more loci. Figure 1: Schematic overview of the all-in-one CRISPR/... work successfully to target multiple sites in cultured human cells. While it has not yet been demonstrated...dCas9-KRAB in our system,” Sakuma said. “These are sure to be powerful tools to modify the expression of...

...Today’s episode of the Addgene Podcast features guest host and Addgene European Outreach Scientist, Benoit...cooperation, and antibiotic production. Chapter 5: The future of Synthetic Biology. Wide adoption of synthetic...Biology and Innovation, Imperial College London and lectures synthetic biology for the Department of Bioengineering...

...University). Right there on the floor outside the lecture hall. It was wonderful to see the ad-hoc ‘sciencing...in vivo using ultrasound (for an example see the figure below). In addition to low costs, deep tissue penetration... imaging of microorganisms in mammalian hosts." Nature 553.7686 (2018): 86.PubMed PMID: 29300010. PubMed...

...more secrets - during a collaboration, members of Maureen Hanson’s and Rima Menassa's labs made the accidental...nm, photoconverted with 405 nm light, and then captured in the red state after excitation at 561 nm. This...conversion did not occur. Tobacco suspension cell culture with cytosol-targeted GFP. White circles represent...

...substantial amount of properly folded, relatively pure protein, but getting to this stage is often much... coli strains designed for protein expression. Cultures are then lysed and the protein of interest is ...disulfide bond formation. pCri also includes a special feature for difficult-to-purify membrane proteins. pCri...