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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 747 results for: UTY

Showing: 701 - 720 of 747 results

  1. Lentivirus Production

    Type
    Protocol
    ...hydrochloric acid until the solution clears. Check the pH of the solution. Use hydrochloric acid or sodium...hydroxide to adjust the pH to 7.0. Typically, the solution will be basic and will need adjustment with hydrochloric...hydrochloric acid first. Pro-Tip The pH of this solution will drift rapidly upon addition of acid or base...over or undershooting the desired pH. Allow the solution to mix for 10 min and then recheck the pH to ensure... ensure that it has not drifted. Filter the solution through a 0.22 μm membrane. Aliquot 500–1000 μL into... tubes at -20 °C or -80 °C. After thawing, the solution can be stored at 4 °C for up to 2 months. After...

  4. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...References Rabinowitz R, Offen D. Single-Base Resolution: Increasing the Specificity of the CRISPR-Cas...

  7. AAV Purification by Iodixanol Gradient Ultracentrifugation

    Type
    Protocol
    ...preparations contain ~20% empty particles. 60% iodixanol solution is available under the name OptiPrep (Link opens...iodixanol and 45 μL of phenol red Underlay each solution into a QuickSeal tube in the order below using...the tube is punctured, AAV-containing Iodixanol solution will flow from the needle set at the 40–60% interface...microcentrifuge tubes are well positioned to collect the solution, or you will lose a significant amount of your...exchange: Stocks of Pluronic F68 Pluronic F68, 10% solution A: 0.1% in PBS: 49.5 mL PBS + 500 µl Pluronic .... Figure 3: Example of an SDS-PAGE gel of 10 consecutive gradient fractions followed by silver stain. ...

  8. Affinity Purification of Recombinant Antibodies with Protein A or Protein G

    Type
    Protocol
    ...should be properly disposed of following your institution’s hazardous waste procedures. Last Update: February...Millipore Sigma S7907-500G 1 M Trizma hydrochloride solution pH 9.0, Millipore Sigma T2819-1L 250 mL sterile...BD302832 Filter, 0.2 µm (luer-lock), VWR 431229 IgG Elution Buffer, Thermo Fisher 21028 Protein G GraviTrap... Warm the affinity column, binding buffer, and elution buffer to room temperature (RT) before use. Reagent... columns at the bottom. Add 5 mL of Pierce IgG Elution Buffer to the capped Gravitrap column and incubate...column at 1000 x g for 2 min to remove storage solution. Place a mark on the side of the column where ...

  13. Personal Protective Equipment (PPE) for BSL-1 and BSL-2 Labs

    Type
    Protocol
    ...includes all of the precautions needed in BSL-1, however there are additional precautions that lie beyond ...infection or disease in healthy humans. Many of the precautions are meant to protect individuals from potential..., but not so tight that you cannot perform your duties. Be sure to try out different sizes to know your... nitrile gloves as an alternative. For extra precautions, tuck your sleeves into your gloves to prevent...

  18. Weighing Reagents Protocol

    Type
    Protocol
    ... you’ll need to make buffers, media, or other solutions. A key part of this task is making sure you’re... weighing all reagents precisely to create the solution you expect. Understanding how to obtain the correct...important to ensure the accurate composition of your solutions and buffers. Now that you have your reagents weighed... weighed out, you can begin making your solutions!...

  19. Pouring LB Agar Plates

    Type
    Protocol
    ...flame. Spray down the bench with a 70% ethanol solution and wipe down with a paper towel. Count out the...molten gel mix, you should create a 1000x stock solution. For example: If you’ll be preparing plates with... 100 ug/mL ampicillin, you should make a stock solution of 100,000 ug/mL (100 mg/mL). Simply measure out...technique. Swirl the agar bottle to ensure even distribution of the antibiotic throughout the agar. Open ...

  20. DNA Quantification

    Type
    Protocol
    ...sure to ‘blank’ the spectrophotometer using the solution the DNA is resuspended in, but with no DNA added...despite the accuracy of the NanoDrop, if two consecutive samples have significantly different concentrations...and consistent readings from DNA in a buffered solution. Reference Page Top Index...
Showing: 701 - 720 of 747 results