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... or affinity tags (HA, FLAG). There are also a variety of plant selection markers (basta, hygromycin, ...

...ddPCR. One issue with qPCR is that the results can vary by a factor of 2. This means that if you set up ...

...systems consist of a genetically encoded enzyme variant tag that reacts specifically and irreversibly with...

... based on the well-studied T7 phage, to target various types of bacteria. In mixed populations, their ...

...between column runs. The TEV protease itself, with various mutations used to increase its stability activity...

...simple and easy technique to implement. Success might vary between experimenters. Vacuum infiltration (Mortensen...

...manipulation. The ReMOT control system (Receptor-Mediated Ovary Transduction of Cargo)  delivers Cas9 RNP to the...

...some of our Addgenies had an idea: sqh mutant fly ovaries had “squiggly” actin fibers that resemble spaghetti...

...control (QC). The specific QC experiments performed varies for each viral lot. To learn which specific QC ...

...ended up being very straightforward to engineer various cell lines from different tissues and mammalian...

...re-use or borrow existing code and control how the various pieces were connected, as this enabled us to rapidly...

...then used this information to identify a large variety of related bacteria with similar Cas systems. Some...

...bacteria. They consist of a toxin which can affect a variety of cellular processes and an antitoxin that suppresses...

...detection: fluorescent protein structure and color variants." Cold Spring Harbor Protocols 2009.12 (2009):...

...featured topic page xCas9: Engineering a CRISPR Variant with Pam Flexibility RNA Editing with Cas13 and...

...Addgene have minor differences due to in silico variations and lack of verification. To address this issue...

...eukaryotes. But be careful — antibiotic mechanisms vary! Aminoglycoside antibiotics also block protein synthesis...

...packages needed to travel meant the accessibility varied country to country.   Figure 1: Number of countries...

...application. Even within an organization, there can be variance. At Addgene, our content team, where writing is...

...karyotypically normal and are able to differentiate into various cell types of all three germ layers [7]. To further...