We narrowed to 776 results for: Vars;

1. PhD Applications After COVID

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   ...are unable to attend in-person interviews for a variety of reasons (logistical, financial, etc…) now have...

2. Hot Plasmids and Viral Preps - May 2021

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   ... Various Addgenies...

3. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

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   ...training from Massachusetts General Hospital and Harvard Medical School. Connect with her on LinkedIn @ ...

4. Tips for a 1st time AAV user (by a Rookie AAV user)

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   ...MOI optimization to account for batch-to-batch variability. Check out this post to learn more about different...

5. 3D Printing Meets CRISPR Cas9

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   ... this interrogation process, they will almost invariably say something like “Oh,…I didn’t quite appreciate...

6. Fluorescent Tagging of Endogenous Genes with SapTrap

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   ...for easy screening of the insertion event, and a variety of connector modules (linker sequences between ...

7. Addgene's Cancer Collection Pages Connect the Research Community

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   .... Despite the prevalence of Ras mutations in a variety of cancers, Ras proteins have stumped the pharmaceutical...

8. Google Forums Round Up: First Impressions of NgAgo

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   ...Update (November 18, 2016): Researchers from a variety of institutions recently reported their inability...

9. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

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   ...biosensors were made with CFP and YFP, cyan and yellow variants of EGFP equally capable of dimerizing as EGFP....

10. PITChing MMEJ as an Alternative Route for Gene Editing

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    ..., and the balance of NHEJ, HDR, and MMEJ repair varies from organism to organism. MMEJ doesn’t yield a...

11. Anatomy of a Plasmid Page at Addgene

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    ...Check the Price of course! The price of some items vary, for example, plasmid kits are avaliable for less...

12. Genome-wide Screening Using CRISPR

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    ...modifying the genome. If you'd like to brush up on the various CRISPR technologies, check out Addgene's CRISPR...

13. Light Sheet Fluorescence Microscopy

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    ... reviews on several other LSFM configurations’ various features see (4,7).   Light sheet fluorescence ...

14. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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    ...BiFC experiments. The ideal control is a mutated variant of one or both of your PIPs in which the protein-interaction...

15. Isolating Genomic Regions of Interest with the CRISPR System

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    ...researchers have begun to make important discoveries in various biological fields. In 2015, the Fujii Lab identified...

16. Viral Vectors 101: The Retroviral Lifecycle

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    ...different genomic sequence from one another) — by various mechanisms, only one of the two strands’ DNA counterparts...

17. Droplet Digital PCR for AAV Quantitation

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    ...ddPCR. One issue with qPCR is that the results can vary by a factor of 2. This means that if you set up ...

18. Tips for arabidopsis transformation

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    ... or affinity tags (HA, FLAG). There are also a variety of plant selection markers (basta, hygromycin, ...

19. Better Dyeing Through Chemistry & Small Molecule Fluorophores

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    ...systems consist of a genetically encoded enzyme variant tag that reacts specifically and irreversibly with...

20. CRISPR Antimicrobials

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    ... based on the well-studied T7 phage, to target various types of bacteria. In mixed populations, their ...