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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 819 results for: cell

Showing: 701 - 720 of 819 results

  1. Adenovirus Guide

    Type
    Guide
    ...enters the host cell, and encode proteins necessary for viral DNA replication and host cell manipulation....expressed by the packaging cell line, normally the human embryonic kidney 293 (HEK293) cell line. There is a rare...transformed into AdEasier-1 cells (Addgene #16399) , which are BJ5183 E. coli cells containing pAdEasy. pAdEasy... construct flanked by ITRs. Cells from the packaging HEK293 or HER911 cell lines expressing E1 are transfected...HER911 cells throughout viral vector production. During experiments, GFP can be used to sort cells infected...transfected into HER911 E4+ cells for rAdV vector production, as HEK293 cells do not contain E4. Download...immune cells and antibody production that develops after exposure to an antigen. AdEasier-1 cells BJ5183...

  2. CRISPR Guide

    Type
    Guide
    ...with Haploid Embryonic Stem Cells Carrying a Guide RNA Library. Cell Stem Cell . 17 (2), 221–232. PMID: ...in the same cell. This is called multiplexing , and increases the likelihood that any cell containing ...can increase efficiency. Synchronizing cells or modulating the cell cycle can help as HDR takes place during...expressing stable cell line. After applying your screening conditions to your transduced cell population, you...tracking of RNA in live cells, including orthogonal RNA/DNA labeling in the same cell. Figure 11: Overview...promiscuity is unique to bacterial cells and does not occur in mammalian cells. SHERLOCK , developed by the ...genomic loci in living human cells by an optimized CRISPR/CAS system. Cell . 155 (7), 1479–1491. PMID: ...

  3. Molecular Biology Reference

    Type
    Guide
    ...daughter cells. These daughter cells contain the same genetic information as the parental cell, and are...with the host cell. Like the bacterial chromosomal DNA, plasmid DNA is replicated upon cell division, and...and each daughter cell receives at least one copy of the plasmid. By the 1970s the combined discoveries...are thus termed clones of the original cell. The plasmid DNA is similarly referred to as cloned DNA, and... and ideally would replicate these plasmids upon cell division in addition to their own genomic DNA. Because...additional replication burden, the rate of bacterial cell division is reduced (i.e., it takes more time to...against the propagation of these plasmids through cell division. To ensure the retention of plasmid DNA...

  10. Chemogenetics Guide

    Type
    Guide
    ...Human Stem Cell-Derived Neurons Repair Circuits and Restore Neural Function. Cell Stem Cell. 28:112-126...targeted to specific tissues, cell types or even subcellular regions of a neuron. Cell-specific expression of...actuators for specific cellular pathways, receptors, or ion channels targeted to specific cell populations (most...signaling, therefore allowing precise control over the cell population they are targeted to. To find chemogenetics...coupled receptors, or GPCRs. The largest class of cell surface receptors, GPCRs are seven-pass transmembrane...Common promoters in chemogenetics plasmids Promoter Cell Specificity hSyn1, CaMKIIa Neurons GFAP Glia CD68... AAV-delivered constructs can be controlled with cell-type specific promoters. Table 4 lists some common...

  11. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    ...Guide RNA and Target DNA. Cell, 156(5), 935–949. https://doi.org/10.1016/j.cell.2014.02.001 Osuka, S., Isomura...efficiency and are important for robust activity in cells (Jiang & Doudna, 2017). Within an interior cavity...it, so many researchers think the enzyme needs cellular recycling factors to release its handiwork (Sternberg...

  12. Optogenetics Guide

    Type
    Guide
    ...subpopulation of cells within the injection site of the viral vector, rather than in all cells. For example...2010 Molecular and cellular approaches for diversifying and extending optogenetics. Cell. 196:1-28. PMID ...and genetic engineering to measure and manipulate cells (frequently neurons) and their governing biomolecular...detect, measure, and control molecular signals and cells in order to understand their functions. Example ...wavelength, the pore opens, cations flow into the cell (yellow dots), and the neuron is activated. Optogenetics...which conducts the flow of ions into or out of the cell. Scientists have identified a variety of naturally...adding trafficking signals to localize opsins to the cell membrane. Microbial opsins, such as those described...

  16. Summer SciComm: Preprints

    Type
    Blog Post
    ...Lacy was also employed by the American Society for Cell Biology in a position working with preprints in ...

  17. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    ...expression in knock-out or knock-down down tissues and cell lines; orthogonal strategies that compare antibody... project aimed at understanding how a critical cellular pathway is regulated. You’ve read all the background...

  19. Addgene: The First Twenty Years

    Type
    Blog Post
    ...Facebook and Gmail, were launched. And Melina Fan, a cell biology graduate student, was frustrated by the ...to receive the Massachusetts Nonprofit Network Excellence Award for our electronic MTA system. We also ...

  20. Antibodies 101: Fab Fragments

    Type
    Blog Post
    ...fragments for multiplexed super-resolution microscopy. Cell Rep Methods 2(10):100301. https://doi.org/10.1016...
Showing: 701 - 720 of 819 results