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  1. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    ...Guide RNA and Target DNA. Cell, 156(5), 935–949. https://doi.org/10.1016/j.cell.2014.02.001 Osuka, S., Isomura...efficiency and are important for robust activity in cells (Jiang & Doudna, 2017). Within an interior cavity...it, so many researchers think the enzyme needs cellular recycling factors to release its handiwork (Sternberg...
  2. Science Guides

    Type
    Guide
    ...receptors to selectively control and investigate cell signaling pathways. Receptors are remotely controlled...and genetic engineering to measure and manipulate cells (frequently neurons) and their governing biomolecular...detect, measure, and control molecular signals and cells in order to understand their functions. Read More...
  3. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    ...expression in knock-out or knock-down down tissues and cell lines; orthogonal strategies that compare antibody... project aimed at understanding how a critical cellular pathway is regulated. You’ve read all the background...
  4. Summer SciComm: Preprints

    Type
    Blog Post
    ...Lacy was also employed by the American Society for Cell Biology in a position working with preprints in ...
  5. Promoters

    Type
    Guide
    ...describes the specifics of these regions in eukaryotic cells. Core Promoter The core promoter region is located...bind. Histones are proteins found in eukaryotic cells that package DNA into nucleosomes. Histone binding...element controls the rate of transcription. Bacterial cells contain sigma factors which assist the RNA polymerase...ribosomal RNA (rRNA) which is a main component of a cell’s ribosome structure. Ribosomes are the site of protein...
  6. Antibodies 101: Fab Fragments

    Type
    Blog Post
    ...fragments for multiplexed super-resolution microscopy. Cell Rep Methods 2(10):100301. https://doi.org/10.1016...
  7. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    ...protein of interest in a complicated solution like cell lysate. But they can be a lot of work — and what...better than a nitrocellulose membrane. If you have already run your blot using nitrocellulose, you may decide...instructions for the membrane you are using. Dry nitrocellulose membranes are brittle, so proceed with caution...
  8. To Each HIS Own

    Type
    Blog Post
    ...This can be pretty complicated given that a single cell has tens of thousands of proteins functioning in...
Showing: 701 - 720 of 812 results