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Addgene
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  1. Finding and Joining Your Dream Lab

    Type
    Blog Post
    ...the current members to share short-term goals and chat about awesome science. Important factors in the ...is the lab? While larger labs tend to be more "chaotic" and offer less micromanaging, they typically offer...

  2. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    ...as the LTRs or structural proteins are not interchangeable between the two systems, other, more general...j.gendis.2017.04.001 Maetzig T, Galla M, Baum C, Schambach A. Gammaretroviral vectors: biology, technology...

  3. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ...recombinase-dependent vectors, this can cause challenges for the experimental design as these vectors ...neurons reside.   Impact for scientists While the mechanisms by which retrograde AAV work are not yet understood...

  4. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    ...manipulations (CRISPR, etc.), and finish with the characterization of a function or phenotype in the surviving...significant survival advantage, such as a non-essential mechanism or any pathway with cellular redundancies, a screen...

  6. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    ...easier to navigate. These practical and effective changes will give you the ability to find the plasmid with... community of scientists) for suggesting these changes, and the SnapGene and Addgene software development...

  8. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    ...and David Root from the Broad Institute and Alex Chavez from the Wyss Institute were instrumental in helping...research and discovery, while also pushing us to challenge assumptions, holding us to nonprofit best practices...

  12. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    ... in your professional circle to vouch for the character you painted in your personal statement. Make certain...hand to get you into that program of your dream.  “Chance favors the prepared mind” by Louis Pasteur These...

  13. Antibodies 101: ChIP

    Type
    Blog Post
    ...such as sequencing or qPCR. Yay!  Where’s the challenge in that?  So while this method is certainly long...the sample type and size.) It can be quite the challenge, and take a few rounds of adjustments, to get ...

  14. Hot Plasmids - February 2022

    Type
    Blog Post
    ...labeling. Here, Liu et al. engineer a chimeric single chain variable fragment (scFv), grafting the binding region...fusion proteins, such as Cas9 and Cas12a, creates challenges for in vivo delivery and cell engineering applications...

  15. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    ...original publication but its name was subsequently changed to clarify that it does not contain the trademarked... information, see the manual provided by the Weiderschain lab. Find Tet-pLKO-puro. lentiGuide-Puro - This...

  16. Addgene: The First Twenty Years

    Type
    Blog Post
    ...MTA system. We also started our blog and YouTube channel in 2013, which continue to grow today. “I generally...Neuroscience 2023 conference. In 2020, the world changed in response to the COVID-19 pandemic, and Addgene...

  17. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ... which target and cleave RNA through the same mechanism as traditional spCas9. If you are looking to modify...it perfect for targeting mRNA and useful as a mechanism of transient knockdown of gene expression. This...

  18. Human Germline Editing Using CRISPR

    Type
    Blog Post
    .... Created with BioRender.com. In later characterization studies, the edited embryos proceeded to the...heterozygous mutations, it will clearly be much more challenging for homozygous mutations. Moreover, introducing...

  19. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    ...fluorescent measurements from red, blue, and green channels overlaid on a bright-field image. Image from Ma...intrachromosomal loci, a major benchmark in characterizing the 4D nucleome. CRISPRainbow: Scaling up to...

  20. What's New in CRISPR - November 2021

    Type
    Blog Post
    ...recently identified Cas12f systems from uncultivated archaea are much smaller and would be ideal for such AAV...system (CasMINI) engineered from the uncultivated archaea Cas12f (Un1Cas12f1, or Cas14a1) system. The CasMINI...
Showing: 701 - 720 of 946 results