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TypeBlog Post...difficult to obtain. These can then be used to perform cell therapy and to model diseases in vitro. Find...et al., Promotion of direct reprogramming by transformation-deficient Myc. Proc Natl Acad Sci U S A, 2010...
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Career Insights: Technical Support Specialist
TypeBlog Post...resources and databases are scrutinized, and this information is enriched with your technical knowledge and...data sets, troubleshooting protocols, analyzing information, and solving customer problems. Technical support... -
An Inside Look into Addgene's Dev Team
TypeBlog Post... these steps, we discover we need additional information. It is quite possible that the clarifications...hows and whys of our users, we are able to make informed software design choices, resulting in software... -
Supporting Reproducibility with a Connected ELN
TypeBlog Post...documents into RSpace, maintaining the original formatting. In this way data is moved from a static and ...conversations and import them into RSpace. The kind of informal conversations about research that can take place... -
The Challenges of Cell Culture
TypeBlog Post...making it easier for comparable studies to be performed on a cell line even decades after it is initially...passage the cells (as well as recording this information in your lab book and on the actual flasks of ... -
Plasmids 101: E. coli Strains for Protein Expression
TypeBlog Post...faciliate proper folding; may increase multimer formation Δ(ara-leu)7697 ΔlacX74 ΔphoA PvuII phoR araD139... T7 RNAP expression alleviates inclusion body formation. Expression vector cannot have p15A origin of ... -
Quick Guide to Near-Infrared Fluorescent Proteins
TypeBlog Post...the probe for their needs. You’ll find basic information on NIR FPs, their properties and applications...mIFP6. Learn more about the characteristics, performance, and applications of near-infrared calcium indicators... -
Tips for Getting a Faculty Position
TypeBlog Post.... I will emphatically deny that I have a magic formula to guarantee a reader the faculty job of his/her... I would not recommend putting these into slide format. You want people to focus on you and your ideas... -
Antibodies 101: Beyond Surface Labeling
TypeBlog Post...day. Note that intracellular labeling must be performed only after viability staining and surface labeling...surface labeling. Intracellular labeling can be performed on cytosolic and/or nuclear markers. Lastly, stains... -
Transferable Skills Guide: Time Management
TypeBlog Post...found that many researchers feel that they lack formal training in a variety of transferable skills. At..., the task will likely be much easier. Having a formal plan for your day will make this easier - if you... -
Filming Science Videos in the Age of Social Distancing
TypeBlog Post... use, but when you need to juggle filming and performing on camera, it’s a lot to handle. We decided the...talent could just focus on their interview or performance. By this point, towards the end of the summer... -
Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods
TypeBlog Post...hour or so, the sample is immediately ready to transform into competent cells. The master mix of enzymes...Gibson assembly master mix for 1 hour, and then transform into competent cells. The success rate of this... -
Exploring Applications of the Bioluminescent HiBiT Tag
TypeBlog Post...also known as KDR), orchestrating blood-vessel formation. Precise, real-time pharmacology of this target...modifications to the binding sites and various isoforms led to subtle changes in co-receptor dynamics,... -
Hot Plasmids: Spring 2025
TypeBlog Post... iGluSnFR3 mutants, identifying the two best-performing variants as iGluSnFR4f (fast deactivation) and...11%. It has higher efficiency and lower indel formation than methods relying on homology-directed repair... -
Viral Vectors 101: AAV Serotypes and Tissue Tropism
TypeBlog Post...of one AAV serotype with the capsid of another. Formally, pseudotypes are described with two numbers separated...using the genomic structure of AAV2, so they are formally called rAAV2/1, rAAV2/2, and so on, although we... -
Antibodies 101: The Basics of Western Blotting
TypeBlog Post...submerged in a tank filled with a transfer buffer formulated to conduct the electric current. The wet transfer...addition, the thickness of the band provides information about the relative abundance of the protein in... -
PiggyBac-ing Through the Genome Editing Field
TypeBlog Post...can be combined with CRISPR/Cas9 to efficiently perform genetic manipulations in animal models or cells...piggyBac™ for gene editing allows you to efficiently perform seamless genetic manipulations, while avoiding ... -
When Fidelity Matters: A frank discussion about ligase fidelity
TypeBlog Post... studies through a high-sensitivity multiplexed format (21, 27). This methodology has been used to rapidly...to screen additional ligases, conditions, and formulations and has led to the development of the new HiFi... -
A Guide to Getting Started in Undergrad Research
TypeBlog Post...scholar/student: visiting from another university to perform research Why should I try research? Personally...reflect on what I wanted for my own future. Informational interviews with people who have taken career... -
CRISPR 101: Multiplex Expression of gRNAs
TypeBlog Post...and Agrobacterium-mediated transformation are available. For more information on this system, check out...