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Showing: 701 - 720 of 961 results

  1. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...standard FPs. The Looger Lab was thus able to easily perform a four-color labeling experiment using smFPs and...References 1. Viswanathan, Sarada, et al. "High-performance probes for light and electron microscopy." Nature...

  2. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...called CROPseq-multi (Walton et al. 2024). This platform is compatible with enrichment, single-cell sequencing...Ace2N-mNeon also led to similar improvements in performance.      Figure 4: Structure of Voltron...

  3. The Challenges of Cell Culture

    Type
    Blog Post
    ...making it easier for comparable studies to be performed on a cell line even decades after it is initially...passage the cells (as well as recording this information in your lab book and on the actual flasks of ...

  4. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...faciliate proper folding; may increase multimer formation Δ(ara-leu)7697 ΔlacX74 ΔphoA PvuII phoR araD139... T7 RNAP expression alleviates inclusion body formation. Expression vector cannot have p15A origin of ...

  5. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...the probe for their needs. You’ll find basic information on NIR FPs, their properties and applications...mIFP6. Learn more about the characteristics, performance, and applications of near-infrared calcium indicators...

  6. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    ... use, but when you need to juggle filming and performing on camera, it’s a lot to handle. We decided the...talent could just focus on their interview or performance. By this point, towards the end of the summer...

  7. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ...day. Note that intracellular labeling must be performed only after viability staining and surface labeling...surface labeling. Intracellular labeling can be performed on cytosolic and/or nuclear markers. Lastly, stains...

  8. Transferable Skills Guide: Time Management

    Type
    Blog Post
    ...found that many researchers feel that they lack formal training in a variety of transferable skills. At..., the task will likely be much easier. Having a formal plan for your day will make this easier - if you...

  9. Tips for Getting a Faculty Position

    Type
    Blog Post
    .... I will emphatically deny that I have a magic formula to guarantee a reader the faculty job of his/her... I would not recommend putting these into slide format. You want people to focus on you and your ideas...

  11. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ...left undisturbed for 13 days. (a, b, c) Colonies formed by expansion of single cells for 13 days. lentiCas9... of a stable cell culture after transduction. Performing antibiotic selection on transduced cells enables...5 dilutions plus one 'no virus' control well). Perform a "reverse transduction" by seeding 50,000 cells...untransduced well (0 µL lentivirus, above) are dying. Perform regular media changes and monitor the growth of...

  12. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ...inverting several times. A white precipitate will be formed which contains the bacterial proteins and genomic...Optional: Perform phenol-chloroform extraction - see protocol below . Note: Phenol-chloroform extraction...mL RNase A TE or water-saturated phenol-chloroform Chloroform 100% ethanol or isopropanol 90% ethanol ...Phenol-Chloroform Extraction of DNA Samples Add an equal volume of TE-saturated phenol-chloroform to the...volume of chloroform to the recovered aqueous DNA layer. Repeat steps 2-4. Note: Phenol-chloroform is a hazardous...purified without a kit it is advisable to perform a phenol/chloroform extraction of the supernatant after step...Bacterial Culture You may also like... Bacterial Transformation Agarose Gel Electrophoresis Agarose Gel DNA...

  13. What is Polymerase Chain Reaction (PCR)

    Type
    Protocol
    ...supplied by the buffer stock, where it is known to form a gradient when frozen. DMSO helps with denaturation...Digest of Plasmid DNA DNA Ligation Background Information A standard Polymerase Chain Reaction (PCR) is...Watch the protocol video below to learn how to perform Polymerase Chain Reaction (PCR). Basic PCR Program...pairs. The initial denaturation step is commonly performed at 94–98°C. Denature 30 seconds at 94°C: Continued...reagent by the number of individual PCRs you wish to perform and add 10% extra to account for pipetting error...

  14. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...submerged in a tank filled with a transfer buffer formulated to conduct the electric current. The wet transfer...addition, the thickness of the band provides information about the relative abundance of the protein in...

  15. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ... iGluSnFR3 mutants, identifying the two  best-performing variants as iGluSnFR4f (fast deactivation) and...11%. It has higher efficiency and lower indel formation than methods relying on homology-directed repair...

  16. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ...of one AAV serotype with the capsid of another. Formally, pseudotypes are described with two numbers separated...using the genomic structure of AAV2, so they are formally called rAAV2/1, rAAV2/2, and so on, although we...

  17. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...can be combined with CRISPR/Cas9 to efficiently perform genetic manipulations in animal models or cells...piggyBac™ for gene editing allows you to efficiently perform seamless genetic manipulations, while avoiding ...

  18. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ...scholar/student: visiting from another university to perform research   Why should I try research? Personally...reflect on what I wanted for my own future. Informational interviews with people who have taken career...
Showing: 701 - 720 of 961 results