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Showing: 701 - 720 of 965 results
  1. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ...of droplets) can be sufficient to capture the information needed for your PCR experiment. The process to...Furuta-Hanawa et al., 2019). Tips and tricks for performing ddPCR Clean everything You must have a clean ...
  2. Great Results Start with Great Standard Curves

    Type
    Blog Post
    ...the standards versus the response and use that information to extrapolate the concentration of an unknown...generated by replicate values provides useful information about the accuracy of the curve, which we will...
  3. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...standard FPs. The Looger Lab was thus able to easily perform a four-color labeling experiment using smFPs and...References 1. Viswanathan, Sarada, et al. "High-performance probes for light and electron microscopy." Nature...
  4. New CRISPR Tools: Cas7-11 and PASTE

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    Blog Post
    ...target. In addition, when they looked at the transformed E. coli expressing Cas7-11, they found no evidence...system as Class I, Type III-E, and then used this information to identify a large variety of related bacteria...
  5. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    ... these steps, we discover we need additional information. It is quite possible that the clarifications...hows and whys of our users, we are able to make informed software design choices, resulting in software...
  6. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ...documents into RSpace, maintaining the original formatting. In this way data is moved from a static and ...conversations and import them into RSpace. The kind of informal conversations about research that can take place...
  7. Career Insights: Technical Support Specialist

    Type
    Blog Post
    ...resources and databases are scrutinized, and this information is enriched with your technical knowledge and...data sets, troubleshooting protocols, analyzing information, and solving customer problems. Technical support...
  8. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...called CROPseq-multi (Walton et al. 2024). This platform is compatible with enrichment, single-cell sequencing...Ace2N-mNeon also led to similar improvements in performance.      Figure 4: Structure of Voltron...
  9. The Challenges of Cell Culture

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    Blog Post
    ...making it easier for comparable studies to be performed on a cell line even decades after it is initially...passage the cells (as well as recording this information in your lab book and on the actual flasks of ...
  10. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...faciliate proper folding; may increase multimer formation Δ(ara-leu)7697 ΔlacX74 ΔphoA PvuII phoR araD139... T7 RNAP expression alleviates inclusion body formation. Expression vector cannot have p15A origin of ...
  11. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...the probe for their needs. You’ll find basic information on NIR FPs, their properties and applications...mIFP6. Learn more about the characteristics, performance, and applications of near-infrared calcium indicators...
  12. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    ... use, but when you need to juggle filming and performing on camera, it’s a lot to handle. We decided the...talent could just focus on their interview or performance. By this point, towards the end of the summer...
  13. Transferable Skills Guide: Time Management

    Type
    Blog Post
    ...found that many researchers feel that they lack formal training in a variety of transferable skills. At..., the task will likely be much easier. Having a formal plan for your day will make this easier - if you...
  14. Antibodies 101: Beyond Surface Labeling

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    Blog Post
    ...day. Note that intracellular labeling must be performed only after viability staining and surface labeling...surface labeling. Intracellular labeling can be performed on cytosolic and/or nuclear markers. Lastly, stains...
  15. Tips for Getting a Faculty Position

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    Blog Post
    .... I will emphatically deny that I have a magic formula to guarantee a reader the faculty job of his/her... I would not recommend putting these into slide format. You want people to focus on you and your ideas...
  16. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...submerged in a tank filled with a transfer buffer formulated to conduct the electric current. The wet transfer...addition, the thickness of the band provides information about the relative abundance of the protein in...
  17. Exploring Applications of the Bioluminescent HiBiT Tag

    Type
    Blog Post
    ...also known as KDR), orchestrating blood-vessel formation. Precise, real-time pharmacology of this target...modifications to the binding sites and various isoforms led to subtle changes in co-receptor dynamics,...
Showing: 701 - 720 of 965 results