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Showing: 701 - 720 of 960 results

  1. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...target. In addition, when they looked at the transformed E. coli expressing Cas7-11, they found no evidence...system as Class I, Type III-E, and then used this information to identify a large variety of related bacteria...

  2. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...called CROPseq-multi (Walton et al. 2024). This platform is compatible with enrichment, single-cell sequencing...Ace2N-mNeon also led to similar improvements in performance.      Figure 4: Structure of Voltron...

  3. The Challenges of Cell Culture

    Type
    Blog Post
    ...making it easier for comparable studies to be performed on a cell line even decades after it is initially...passage the cells (as well as recording this information in your lab book and on the actual flasks of ...

  4. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...faciliate proper folding; may increase multimer formation Δ(ara-leu)7697 ΔlacX74 ΔphoA PvuII phoR araD139... T7 RNAP expression alleviates inclusion body formation. Expression vector cannot have p15A origin of ...

  5. Transferable Skills Guide: Time Management

    Type
    Blog Post
    ...found that many researchers feel that they lack formal training in a variety of transferable skills. At..., the task will likely be much easier. Having a formal plan for your day will make this easier - if you...

  6. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...the probe for their needs. You’ll find basic information on NIR FPs, their properties and applications...mIFP6. Learn more about the characteristics, performance, and applications of near-infrared calcium indicators...

  7. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    ... use, but when you need to juggle filming and performing on camera, it’s a lot to handle. We decided the...talent could just focus on their interview or performance. By this point, towards the end of the summer...

  8. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ...day. Note that intracellular labeling must be performed only after viability staining and surface labeling...surface labeling. Intracellular labeling can be performed on cytosolic and/or nuclear markers. Lastly, stains...

  9. Tips for Getting a Faculty Position

    Type
    Blog Post
    .... I will emphatically deny that I have a magic formula to guarantee a reader the faculty job of his/her... I would not recommend putting these into slide format. You want people to focus on you and your ideas...

  11. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...submerged in a tank filled with a transfer buffer formulated to conduct the electric current. The wet transfer...addition, the thickness of the band provides information about the relative abundance of the protein in...

  12. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ... iGluSnFR3 mutants, identifying the two  best-performing variants as iGluSnFR4f (fast deactivation) and...11%. It has higher efficiency and lower indel formation than methods relying on homology-directed repair...

  13. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ...of one AAV serotype with the capsid of another. Formally, pseudotypes are described with two numbers separated...using the genomic structure of AAV2, so they are formally called rAAV2/1, rAAV2/2, and so on, although we...

  14. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...can be combined with CRISPR/Cas9 to efficiently perform genetic manipulations in animal models or cells...piggyBac™ for gene editing allows you to efficiently perform seamless genetic manipulations, while avoiding ...

  16. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ...scholar/student: visiting from another university to perform research   Why should I try research? Personally...reflect on what I wanted for my own future. Informational interviews with people who have taken career...

  19. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ... minimal limitations. Background In its simplest form, PCR based cloning is about making a copy of a piece...recipient plasmid backbone. Transformation Proceed with the transformation according to the manufacturer...from your transformation will give you the first indication as to whether your transformation worked. Your...Digest of Plasmid DNA DNA Ligation Bacterial Transformation Summary PCR based cloning is incredibly versatile...see (Link opens in a new window) NEB for more information). Thus, we recommend that you add 3-6 bases upstream...should ensure that the bases do not result in the formation of a hairpin structure within your primer. In ...competent cells. For most standard cloning, you can transform 1-2μl of your ligation reaction into competent...

  20. Pouring LB Agar Plates

    Type
    Protocol
    ...our case 220 mL) to the same bottle and swirl to form a medium/agar colloid. Cover the opening of the ...lab. The addition of agar to LB results in the formation of a gel that bacteria can grow on, as they are...
Showing: 701 - 720 of 960 results