We narrowed to 997 results for: lars;

...generating a frameshift mutation. The majority of cellular repair events will generate these types of mutations...hundred bps of sequence. However, if you have a larger knock-in (200 bp – 2 kb), a dsDNA donor will need...oligonucleotides. These donors traditionally have larger homology arms in the 500 bp to 1 kb range, however...

...beautifully compelling photos and videos, while molecular, micro, and cell biology are often more suited...microscopy) Visually compelling. Easy to convey a large number of details without overwhelming your audience...Difficult to do in-depth storytelling. Requires regular content production. Internet "trolls"   More...

...Addgene, as a proxy for global activity in our particular scientific subfields.  Plasmid requests correlate...plasmid requests correspond to publications of popular papers. Figure 1: Plasmid requests by date...-examine our global data at a finer temporal granularity. For each paper, we observed that the demand ...

...journal articles. From paper to plasmid Authors regularly indicate that their plasmids are available from... very likely find a useful plasmid, but what’s popular? The coveted Blue Flame is more than an award for... Tags: Identify a specific marker protein Use: Similar to “Vector Type” you can filter by experimental...

...vectors are replication-deficient. Thus, a very large number of phagemids would be needed for an in vivo...Diana P. Pires, and Timothy K. Lu. “Engineering Modular Viral Scaffolds for Targeted Bacterial Population...Methods for Bacterial Genome Engineering Treating Muscular Dystrophy with CRISPR Gene Editing Using CRISPR...

...known to have replicons with broad host ranges. Popular replicons from these groups include: RK2 (IncP)...vectors for gene expression in bacteria. Methods in Molecular Biology (Clifton, N.J.), 765, 327–343. https:/...endonucleases on the broad host-range plasmid RK2. Molecular & General Genetics: MGG, 152(3), 129–135. https...

...are not always positioned correctly to target a particular gene. Furthermore, a target sequence may have... desired target locus. The PAM sequence is of particular concern when trying to edit a gene using homology...cleavage events for the VQR and VRER variants is similar to wild-type SpCas9, indicating that the variants...

...level of Gene X in cells treated with Plasmid A is similar to the expression level of Gene X in the untreated... controls are negative controls that produce a similar product, but do not target an endogenous gene in... variable and helps ensure the phenotype isn’t singular or associated with only one aliquot/preparation...

... Service Viral Vector Guides Virus Blog Posts Molecular Biology Reference Introduction This protocol describes... lower or higher dilutions depending on their particular sample. For additional tips on AAV titering using... bucket 96-well freezer blocks (x 3) Reagents Molecular Biology Grade Water, Hyclone, SH30538.02 GeneAmp...10X Buffer 25 µL of 10% Poloxamer 188 4475 µL Molecular Biology Grade Water Procedure Preparation Before...

... matter how many times I say this, candidates regularly try to convince me that their search committee...whiskies, but I remember the days of modest postdoc salaries all too well. If there's a group to make money...search!   Erik Lee Snapp received his PhD in Molecular Microbiology and Immunology from Oregon Health...

...functional screen of your liking. This method is similar to site-directed mutagenesis (SDM) PCR, but with...Random Mutagenesis by Error-Prone PCR”. Methods in Molecular Biology In Vitro Mutagenesis Protocols, pg. 103...Measuring the Activity of Protein Variants on a Large Scale Using Deep Mutational Scanning.” Nature Protocols...

..., but I always seek out information about the particular audience and venue before tailoring each presentation...peer mentors to check your slides for typos and clarity in a big room.  Finally, don’t pass up opportunities...informal poll by asking for a show of hands on a particular question. I like to ask about how many people...

...efficiency differed from 48-100% between strains. In a similar experiment, CRISPR reduced the HIV-1 copy number...evidence that an HIV-1 cure may be within our reach. Similar studies have shown that CRISPR can be used to combat...Additional Resources on the Addgene Blog Treating Muscular Dystrophy with CRISPR Gene Editing Learn to Use...

...appropriate secondary antibodies. The general process is similar to standard immunofluorescence, but with some extra...selecting an antibody, some of the requirements are similar to standard IF. Here, we’ll walk you through the...if the localization and intensity patterns are similar between the two experiments. If not, it might be...

...Mentorship Program work? IMP Pilot Program 2015-2015 Scholars need strong proactive mentors who have relevant...President from the non-profit IMFAHE, Inc. She is particularly interested in breast cancer and metastasis as... Medical School, where she studies age-related macular degeneration. She is also a blogger who writes ...

... to choose from that sequence preferences must largely be ignored. Created with BioRender.com. ...nuclease-mediated off-target effects.” Further, large-scale datasets of hundreds of genetic screens using...Tsherniak A, Iorio F (2019) Agreement between two large pan-cancer CRISPR-Cas9 gene dependency data sets...

...reason most people enter postdoctoral training. Similarly, there are several factors to consider when selecting...to strengthen your new model?  PFs need to be regularly thinking about manuscripts. The other items will...Erik Snapp! Erik Lee Snapp received his PhD in Molecular Microbiology and Immunology from Oregon Health...

...samples. This is a commonly used technique for molecular cloning, such as PCR - or restriction enzyme -...you get better separation of bands? If you have similarly sized bands that are running too close together..., and a lower percentage gel will help separate larger bands. 10% Rule For each sample you want to load...

...result in similar sized bands, thus making this simple digest less informative. This is particularly true ...occurring bacterial endonucleases that recognize a large range of DNA sequences. Given the variety of these...

...less efficient at taking up larger plasmids. If you need to transform large plasmids, it is a good idea...very few if any transformants when transforming large plasmids (>10 kb) or BACs, what can I do? Chemically...