We narrowed to 780 results for: mos
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TypeBlog Post...Flow cytometry is one of the most powerful tools available to immunologists, allowing for the rapid analysis...
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Viral Vectors 101: Producing Your rAAV
TypeBlog Post...simplex virus to facilitate efficient replication. The most common production system, however, is a “helper-... -
PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs
TypeBlog Post...PRIDICT saves time and resources by selecting the most promising pegRNA designs and especially facilitates... -
Hot Plasmids and Viral Preps - May 2021
TypeBlog Post...RBD responsible for binding ACE2 is the target of most anti-SARS-CoV-2 antibodies. In the library, each... -
Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity
TypeBlog Post...NEB (1, 2). While T4 DNA Ligase, the mainstay of most biotechnological cloning efforts for over 50 years... -
PITChing MMEJ as an Alternative Route for Gene Editing
TypeBlog Post...termed PITCh (Precise Integration into Target Chromosomes). Using their PITCh plasmids, GFP knock-in cell... -
CRISPR 101: Mammalian Expression Systems and Delivery Methods
TypeBlog Post...protein such as GFP? All of these outcomes can be most effectively achieved with different CRISPR components... -
Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments
TypeBlog Post...Institute) has consistently been one of the repository’s most frequently requested plasmids. I wondered how many... -
Isolating Genomic Regions of Interest with the CRISPR System
TypeBlog Post...numerous genomic loci through proteomics and the chromosome conformation capture (3C) technique. In addition... -
INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons
TypeBlog Post... R, Jiang Y, Yang J, Yang S (2020) Multicopy Chromosomal Integration Using CRISPR-Associated Transposases... -
CRISPR 101: RNA Editing with Cas13
TypeBlog Post...42% editing efficiency. They note that RESCUE is most active in a 30 nt guide when C or U base-flips are... -
AAVs CREATed for Gene Delivery to the CNS and PNS
TypeBlog Post... Adeno-associated viral (AAV) vectors are the most frequently used gene-transfer tools in the study... -
Negative Can Be Positive: Open AAV Data with Addgene
TypeBlog Post...available to evaluate AAV vector performance. Yet, most of the technical data needed to replicate and build... -
Deep Dive: Fixing and Permeabilizing for Immunofluorescence
TypeBlog Post...cellular and tissue morphology. Formaldehyde is the most commonly used cross-linker for IF. However, you ... -
Course-Based Undergraduate Research Experiences (CUREs)
TypeBlog Post...), decided to take a different approach. His own most impactful learning experiences happened in the lab... -
CRISPR 101: Making a Knock-In Cell Line
TypeBlog Post... other edits Like I said earlier, HDR is not the most common editing pathway for DNA repair. To tilt the... -
Droplet Digital PCR for AAV Quantitation
TypeBlog Post...virus in reaction mix D = Dilution factor of virus Most droplet readers have a few channels for detecting... -
Tips for arabidopsis transformation
TypeBlog Post... region, it may cause unexpected phenotypes. For most phenotypic analysis it is appropriate to begin working... -
Simplify Cloning with in vivo Assembly
TypeBlog Post...common lab cloning strain has failed so far. What is most important is the transformation efficiency of the... -
New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries
TypeBlog Post...overexpression library identified several overlapping genes, most genes were unique to the individual libraries. These...