We narrowed to 780 results for: mos

1. Antibodies 101: Conventional vs Spectral Flow Cytometry

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   Blog Post

   ...Flow cytometry is one of the most powerful tools available to immunologists, allowing for the rapid analysis...

2. Viral Vectors 101: Producing Your rAAV

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   Blog Post

   ...simplex virus to facilitate efficient replication. The most common production system, however, is a “helper-...

3. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

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   Blog Post

   ...PRIDICT saves time and resources by selecting the most promising pegRNA designs and especially facilitates...

4. Hot Plasmids and Viral Preps - May 2021

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   Blog Post

   ...RBD responsible for binding ACE2 is the target of most anti-SARS-CoV-2 antibodies. In the library, each...

5. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

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   Blog Post

   ...NEB (1, 2). While T4 DNA Ligase, the mainstay of most biotechnological cloning efforts for over 50 years...

6. PITChing MMEJ as an Alternative Route for Gene Editing

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   Blog Post

   ...termed PITCh (Precise Integration into Target Chromosomes). Using their PITCh plasmids, GFP knock-in cell...

7. CRISPR 101: Mammalian Expression Systems and Delivery Methods

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   Blog Post

   ...protein such as GFP? All of these outcomes can be most effectively achieved with different CRISPR components...

8. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

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   Blog Post

   ...Institute) has consistently been one of the repository’s most frequently requested plasmids. I wondered how many...

9. Isolating Genomic Regions of Interest with the CRISPR System

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   Blog Post

   ...numerous genomic loci through proteomics and the chromosome conformation capture (3C) technique. In addition...

10. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

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    Blog Post

    ... R, Jiang Y, Yang J, Yang S (2020) Multicopy Chromosomal Integration Using CRISPR-Associated Transposases...

11. CRISPR 101: RNA Editing with Cas13

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    Blog Post

    ...42% editing efficiency. They note that RESCUE is most active in a 30 nt guide when C or U base-flips are...

12. AAVs CREATed for Gene Delivery to the CNS and PNS

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    Blog Post

    ...   Adeno-associated viral (AAV) vectors are the most frequently used gene-transfer tools in the study...

13. Negative Can Be Positive: Open AAV Data with Addgene

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    Blog Post

    ...available to evaluate AAV vector performance. Yet, most of the technical data needed to replicate and build...

14. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

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    Blog Post

    ...cellular and tissue morphology. Formaldehyde is the most commonly used cross-linker for IF. However, you ...

15. Course-Based Undergraduate Research Experiences (CUREs)

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    Blog Post

    ...), decided to take a different approach. His own most impactful learning experiences happened in the lab...

16. CRISPR 101: Making a Knock-In Cell Line

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    Blog Post

    ... other edits Like I said earlier, HDR is not the most common editing pathway for DNA repair. To tilt the...

17. Droplet Digital PCR for AAV Quantitation

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    Blog Post

    ...virus in reaction mix D = Dilution factor of virus Most droplet readers have a few channels for detecting...

18. Tips for arabidopsis transformation

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    Blog Post

    ... region, it may cause unexpected phenotypes. For most phenotypic analysis it is appropriate to begin working...

19. Simplify Cloning with in vivo Assembly

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    Blog Post

    ...common lab cloning strain has failed so far. What is most important is the transformation efficiency of the...

20. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

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    Blog Post

    ...overexpression library identified several overlapping genes, most genes were unique to the individual libraries. These...