We narrowed to 932 results for: omp
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TypeBlog Post...epitopes available if it is being selected out of a complex mix of proteins that have been extracted from a...Picking the Right Antibody It can get even more complex if you’re looking for proteins that are interacting...
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Hot Plasmids and Viral Preps - September 2021
TypeBlog Post...to 266 μg/mL. The reaction only required three components: a master mix, the DNA of interest, and cell ...several pooled libraries: Cas13X and Cas13Y, two compact Cas13 proteins found in the metagenome datasets... -
Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC
TypeBlog Post...targets. Pre-formed primary and secondary antibody complexes (Figure 2B) Some labs have found that you can ...). One issue with this strategy is that large complexes may not permeate through your tissue samples as... -
Donations from Addgene to Yield Answers for Rare Disease Researchers
TypeBlog Post...the Rare Genomics Institute sponsors a global competition for researchers working on rare diseases, offering...paralysis (hemiplegia) that are often painful and accompanied by migraines. Those attacks can be brought on... -
Hot Plasmids: FRET-Based Biosensors
TypeBlog Post...interest,” Griesbeck said. Because they are chemical compounds, they have to be applied or injected, and they...give it a shot. “Beginners sometimes think it’s complicated, but actually it is very good,” he said. “It ... -
Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering
TypeBlog Post...the cells within them work together or perhaps compete against each other. Getting two-color stimulation...three or four hour conversation. The brain is very complex and we've hosted hundreds of visitors in our lab... -
Scientists Map the SARS-CoV-2-Human Interaction Network
TypeBlog Post...published an article on BioRxiv describing a comprehensive SARS-CoV-2 virus-human interaction network in... are a few key details. Our set of constructs comprises all SARS-CoV-2 viral proteins except for Nsp3 ... -
"Build Your Plasmid": The Game - Play to Teach
TypeBlog Post...playing a card game called “Magic”. There were complicated rules that governed how this game was played....should be “brains-on”, but as much as I try being compelling, interactive, and application-driven to boost... -
Celebrate National Book Month with Addgene
TypeBlog Post...Musicophilia is accessible and fascinating, all without compromising on the relevant science." - Randall Carter...Additional resources on the Addgene blog Learn about company culture at Addgene Read about other lunch activities... -
Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System
TypeBlog Post...would recognize serotonin. Using a combination of computer modeling, directed evolution, site-saturation ...and colonization opportunities for bacteria. To compare the artificial leaf to A. thaliana leaf, Bernach... -
Visualizing Genomic Loci with CRISPR-Sirius
TypeBlog Post...and sgRNA-Sirius-8XMS2 scaffolds performed, they compared them head-to-head against CRISPRainbow. Both tools...visualization tools can be used in live cells. Compared to its predecessor CRISPRainbow, CRISPR-Sirius... -
The Crispr Babies Saga Shows the Need for Action, Not More Delays
TypeBlog Post...techniques are continually being refined; it is completely plausible that scientists will eventually have...standardized process of good research practice: computer simulations, in vitro research, animal models,... -
Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS
TypeBlog Post...mutation that disrupts the function of YFG. You could compare this mutant fly to one lacking the mutation in ...wanted to express them within a specific neuronal compartment, you could express them individually or simultaneously... -
The Power Behind NGS Plasmid Validation: seqWell
TypeBlog Post...custom primers or "primer-walking" reactions are completely unnecessary for sequencing multi-kb DNA inserts...seqWell, Inc. He is a sequencing technologist and computational biologist with a passion for solving critical... -
The MTA at Addgene
TypeBlog Post...has placed a request for your plasmid, they must complete a Recipient Scientist Acknowledgment (RSA), which...use for the requested material. Once the RSA is completed, the recipient organization is sent the MTA to... -
Hot Plasmids - January 2023
TypeBlog Post... Fig. 1: Csm, shRNA, and Cas13 were compared head-to-head for gene expression changes of non-target...JA. Precise transcript targeting by CRISPR-Csm complexes. Nat Biotechnol. 2023 Jan 23. doi: 10.1038/s41587... -
Networking Follow-up: I Met a Person. Now What?
TypeBlog Post...link can be your new card. Follow your contact’s company on LinkedIn (or via industry newsletters such as...this because I want it to be clear that it is completely impossible for me or anyone to stay in touch ... -
With Our New Viral Service, We're Taking CRISPR Further!
TypeBlog Post... production of viruses used to deliver CRISPR components to cells or organisms under study. In the past...lentivirus production frustrating - a researcher can complete an entire lentiviral production protocol only ... -
Four Factors that Differentiate the Stem Cell Field
TypeBlog Post...field. Of course they are competing with each other, but it's a healthy competition. What an honor for Addgene... -
Plasmids 101: Screening Strategies Used in Plasmid Cloning
TypeBlog Post... creates a non-functional 𝛽-galactosidase. Complementing this mutation by expressing this deleted portion...get started, you will first need a primer that complements your plasmid sequence. Start with a backbone-...