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We narrowed to 996 results for: ran

Showing: 701 - 720 of 996 results

  1. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...the embryos underwent repair not from the single-stranded oligonucleotide (ssODN) template supplied by Ma...maternal allele rather than the supplied single-stranded oligonucleotide (ssODN) template. Embryos also...off-target effects, but this advantage likely won’t transfer to all genes we’d like to target. In addition,...

  3. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    ...poison, locking up DNA gyrase with broken double stranded DNA and ultimately causing cell death. ccdA, another...gene in frame with an MCS. E. coli that were transformed with the empty vectors expressed the ccdB gene... that ccdB Survival™ can be more difficult to transform, depending upon the specific plasmids being used...

  4. Addgene: The First Twenty Years

    Type
    Blog Post
    ...Part of that work meant facilitating the Material Transfer Agreement process, which was a major barrier for... for depositors and requestors alike. We transformed this process by aligning institutions with standardized...Addgene. I think resources like this are why my brand of neuroscience has exploded. Because we have a ...

  6. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...neurons from each class to fully characterize the transcriptome. The first data release for CeNGEN is planned... The 3’UTRs of mRNA are important for post-transcriptional regulation in C. elegans. To study the role...using a split intein for refined spatiotemporal transgene control in Caenorhabditis elegans." Proceedings...

  7. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    ...loop-mediated isothermal amplification after reverse transcription (RT-LAMP) and a colorimetric readout that can... 45 minutes. The team deposited the reverse transcriptase, protease, and Bst polymerase constructs from...toolkit include ones for expressing MMLV reverse transcriptase and Taq polymerase that can be used in a one-step...

  8. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

    Type
    Blog Post
    ...Interactions Difficult to detect weak and/or transient PPIs. Many PPI identification techniques rely ...often prevents the identification of weak and/or transient interacting proteins. No spatiotemporal control...BioID, is also useful for identifying weak and/or transient protein-protein interactions since the biotin ...

  10. How to Prepare for an Industry Interview

    Type
    Blog Post
    ...position, I just had a phone interview. I also transitioned between different lab groups without having ... an opportunity to demonstrate technical and transferable skills that make you qualified for the position...experience less stressful. First, you can make arrangements with those you live with to have a quiet place...

  11. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

    Type
    Blog Post
    ...that the TadA domain can edit the short single-stranded DNA stretch that is accessible. Image from Gaudelli... protospacer, the editing capacity of the ABE8s ranged from 1.5x higher for positions A5-A7 to 3.2x higher...References Gaudelli NM, Komor AC, Rees HA, Packer MS, Badran AH, Bryson DI, Liu DR (2017) Programmable base ...

  12. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ...pipetting error or an incredible discovery. The range of RQ values used to call copy numbers should be...fluorescent dye that works by binding to all double-stranded DNA, while Taqman uses an intercalating dye, with...confident determination of copy number, albeit within a range of RQ values as noted above.   In both applications...

  13. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ... lab has created a novel nanocompartment for transporting proteins in yeast by using murine polyomavirus...2A-Synaptophysin-mRuby from Liqun Luo’s lab expresses membrane tethered GFP to enhance axonal labeling and synaptophysin-tagged...expressing GCaMP6f with this promoter. Image calcium transients in PV interneurons with AAV-S5E2-GCaMP6f are ...

  14. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    ...of the endonuclease Cas9 to create a DNA double stranded break (DSB) at a genomic site specified by a guide...tag. The two PCR reactions are then mixed and transfected along with a Cas9 expression vector. They can...interactions. Additionally, thanks to the modular arrangement of vectors included in tagging systems like SapTrap...

  15. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    ...to choose  Where is your audience?  It’s, quite frankly, much easier to go where your audience is than ...resources do you have?  No matter the project, I can guarantee you that either resource or time restrictions ...competitive while digital can be oversaturated. Transition from academic to informal writing can difficult...

  16. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...jam packed with everything needed for synaptic transmission, are right around 250 nm, making it difficult...pre-expanded samples can sometimes take on a fragmented appearance post-expansion, but that doesn’t mean we should...brief history and new avenues. Philosophical Transactions Royal Soc 380:20210110. https://doi.org/10.1098...

  17. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ...). But is it possible to bring the kinds of transformative educational experiences afforded to those fortunate... now connects MDH-focused CUREs at 50 schools, ranging from R1 institutions to community colleges, and...NADH production.   CUREs are beginning to transform undergraduate education in the sciences by making...

  18. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ...diligent scientist, they sought to understand these strange results by digging deeply into their data.   ...Samples are divided into those of African ancestry(orange) and those of other ancestry groups (grey). ...): 250-252. Carlson J, Henn BM, Al-Hindi DR, Ramachandran S. Counter the weaponization of genetics research...

  19. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type
    Blog Post
    ...enhancers and genome math come in. They help us to transform raw genomic coordinates into meaningful experiments...“GPS for proteins”) for NANOG, an essential transcription factor in embryonic stem cells (ESCs). Your ...as a linear polymer of A,T,G, and C nucleotides arranged in a double helix. People are wrong. In reality...

  20. Plasmids 101: Colony PCR

    Type
    Blog Post
    ...control. A good positive control is bacteria transformed with the same backbone plasmid. If this control...strain. A good negative control strain is an untransformed culture of the same strain of bacteria you used...Cloning Technique Learn to Perform a Bacterial Transformation Deposit Your Positive Clones with Us ...
Showing: 701 - 720 of 996 results