Hot Plasmids - November 2023
Type
Blog Post
...Credit: Zhuhao Wu, Weill Cornell Medicine.
Interested in this antibody, but wish it were from a different...
We narrowed to 759 results for: rest
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Transferable Skills Guide: Creativity
TypeBlog Post...exercise: Consider the issue as if there were no restraints on your problem-solving resources. What would... -
pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs
TypeBlog Post... Massachusetts General Hospital with research interests in the fields of gene editing and stem cells. ... -
Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone
TypeBlog Post...they naturally find people with which they have interests and experiences in common. We often refer to this... -
Neuronal labeling with Spaghetti Monster
TypeBlog Post...antigenic peptide sequences, attached to a protein of interest (POI), that facilitate immunohistochemistry (IHC... -
Changing Labor Laws Bring Increased Postdoc Wages
TypeBlog Post...Executive Director of Future of Research. He is interested in advocating for and with junior scientists,... -
Healthcare Consulting: A Door to the Business of Life Sciences
TypeBlog Post...works as a healthcare consultant in Boston. He is interested in the business of life sciences and it's impact... -
CRISPR Activation: A Practical Guide
TypeBlog Post... you can achieve using a CRISPRa. Your gene of interest might already be hitting an upper bound of activation... -
Tips for CRISPR Gene Editing in Mice
TypeBlog Post... delete the entire gene? Perhaps you are more interested in the c-terminus and would like to target that... -
The Challenges of Cell Culture
TypeBlog Post... there would be no viable viruses to study. Interestingly, due to certain high profile problems surrounding... -
Viral Vectors 101: Integrase-Deficient Lentiviral Vectors
TypeBlog Post...lentiviral transfer plasmid encodes your insert of interest, flanked by long terminal repeat (LTR) sequences... -
An Integrin Antibody Toolkit from IPI
TypeBlog Post...preferences of the αV subunit and its β partners. Of interest, we demonstrated that anti-integrin αVβ5.9 “essentially... -
Transferable Skills Guide: Time Management
TypeBlog Post...Gilmore directly to see if he knows anyone who’s interested in meeting Send out as many emails as possible... -
Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus
TypeBlog Post..., the partial genomes complement each other to restore the full-expression cassette. AAV’s natural ability... -
3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells
TypeBlog Post...reads that we studied by deep sequencing, but interestingly (and to our frustration) in many cases we only... -
Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?
TypeBlog Post...viral protein production, so researchers are also interested in precisely excising the HIV-1 genome from infected... -
How to Negotiate a Successful Lab Start Up Budget
TypeBlog Post...This is actually why a department/institute is interested in you. Your science will define your success... -
Hot Plasmids: Spring 2025
TypeBlog Post...promoter, tag, polyA site) are flanked by unique restriction sites so you can swap sequences effortlessly.... -
CRISPR 101: Off-Target Effects
TypeBlog Post...making specific knock-out edits in a gene(s) of interest and has an average off-target frequency of 5%.... -
Viral Vectors 101: Optogenetic Tools
TypeBlog Post...entire signaling pathways, but what if you’re interested in studying signaling pathways? Here’s where ...