QC Sequencing Technologies at Addgene
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Blog Post
...choice for small sample sizes, small regions of interest, or complex sequences such as G-C rich areas, ...
We narrowed to 759 results for: rest
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Hot Plasmids: Fall 2024
TypeBlog Post...brightness (such as low-expression proteins of interest) and mScarlet-I3 for scenarios requiring rapid... -
Plasmids 101: Stringent Regulation of Replication
TypeBlog Post...levels low even if plasmid copy numbers are high, restraining further replication. Figure 3: Comparison... -
Hot Plasmids Spring 2024
TypeBlog Post...extraneous genetic material to reconstitute the gene of interest, reducing the immunogenicity typically associated... -
Hot Plasmids - November 2023
TypeBlog Post...Credit: Zhuhao Wu, Weill Cornell Medicine. Interested in this antibody, but wish it were from a different... -
Changing Labor Laws Bring Increased Postdoc Wages
TypeBlog Post...Executive Director of Future of Research. He is interested in advocating for and with junior scientists,... -
Healthcare Consulting: A Door to the Business of Life Sciences
TypeBlog Post...works as a healthcare consultant in Boston. He is interested in the business of life sciences and it's impact... -
CRISPR Activation: A Practical Guide
TypeBlog Post... you can achieve using a CRISPRa. Your gene of interest might already be hitting an upper bound of activation... -
Tips for CRISPR Gene Editing in Mice
TypeBlog Post... delete the entire gene? Perhaps you are more interested in the c-terminus and would like to target that... -
The Challenges of Cell Culture
TypeBlog Post... there would be no viable viruses to study. Interestingly, due to certain high profile problems surrounding... -
Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus
TypeBlog Post..., the partial genomes complement each other to restore the full-expression cassette. AAV’s natural ability... -
3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells
TypeBlog Post...reads that we studied by deep sequencing, but interestingly (and to our frustration) in many cases we only... -
Transferable Skills Guide: Time Management
TypeBlog Post...Gilmore directly to see if he knows anyone who’s interested in meeting Send out as many emails as possible... -
Viral Vectors 101: Integrase-Deficient Lentiviral Vectors
TypeBlog Post...lentiviral transfer plasmid encodes your insert of interest, flanked by long terminal repeat (LTR) sequences... -
An Integrin Antibody Toolkit from IPI
TypeBlog Post...preferences of the αV subunit and its β partners. Of interest, we demonstrated that anti-integrin αVβ5.9 “essentially... -
Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?
TypeBlog Post...viral protein production, so researchers are also interested in precisely excising the HIV-1 genome from infected... -
How to Negotiate a Successful Lab Start Up Budget
TypeBlog Post...This is actually why a department/institute is interested in you. Your science will define your success... -
Is this the right place for me? 8 tactics for choosing a lab
TypeBlog Post... aspects) applicable to industry if this path interests you (e.g. human disease or models of disease,... -
Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility
TypeBlog Post...description to watch any sections you’re particularly interested in, but I’d recommend watching the whole livestream... -
Hot Plasmids: Spring 2025
TypeBlog Post...promoter, tag, polyA site) are flanked by unique restriction sites so you can swap sequences effortlessly....