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We narrowed to 957 results for: tes

Showing: 701 - 720 of 957 results
  1. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    ...parts: the receptor itself and the G-proteins it associates with.  Receptor: GPCRs are also known as seven...transducing receptors in the brain they are ideal candidates for use in chemogenetics. To create chemogenetics...
  2. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ...site of interest, which nCas9 nicks and then dissociates from. PolI3M then binds the nicked DNA, and extends..., where it nicks its target DNA (left). Cas9 dissociates and PolI binds (middle) and extends from the ...
  3. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    ... and have worked closely with us to functionally test our virus. Ed Boyden’s lab from MIT and Alla Karpova... methods, performs extensive quality control, generates  educational materials, and provides viral vector...
  4. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    ...idea of the basics of immunoimaging. A scientist conjugates an antibody with a signaling molecule, the antibody...secondary antibodies on hand and use them to easily test different antibodies or different signaling molecules...
  5. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ...as KLH or Freund’s adjuvant is injected, which stimulates the overall immune response. Once the initial...weeks to increase the antibody titer. The titer is tested following each immunization until the desired level...
  6. Addgene: The First Twenty Years

    Type
    Blog Post
    ...theaters, the Razr was the hottest phone on the market, and two new websites, Facebook and Gmail, were ...
  7. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ...express genes or gene sets over 4.7 kb. Co-infection rates for AAVs are generally high enough for this approach... guest blogger Didem Goz Ayturk with edits and updates from Addgenies Karen Guerin and Susanna Stroik....
  8. The time and cost required to make a plasmid

    Type
    Blog Post
    ...reagents and consumables, especially for things like plates and gels, which either don’t scale or have limited...my ability to tell. But even from these rough estimates, it’s clear that community engagement with and...
  9. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    ...load The Wikipedia definition of cognitive load states: “cognitive load refers to the used amount of working...even the most enthusiastic of learners.  What contributes to cognitive load?  Everything! Here are some...
  10. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...and isolated with antibiotic selection. The team tested this system in vitro and in vivo. They identified...fluorescent proteins into one bright particle that segregates into one cell during cell division. To achieve...
  11. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    ...(Japanese, German, and English) and even on 60 Minutes. We are blessed with a large community of scientists... employee pool and we get a lot of successful candidates from internal referrals. Our long reaching reputation...
  12. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...below): 1. Resistant cells among the separate replicates would contain the same labels - i.e. they would...subpopulation 2. Resistant cells among the separate replicates would have different labels - i.e. they would...
  13. Optogenetics Guide

    Type
    Guide
    ...Experiment References The field of optogenetics integrates optics and genetic engineering to manipulate ...photocurrent compared to CrChR2 450 ChETA E123T mutation; creates faster kinetics but reduces photocurrent amplitude... msec with hChR2 to "long lasting" (seconds to minutes) with stable step-function opsins (SSFOs). Delivery...flavin mononucleotide as its chromophore, and phosphorylates the transcriptional activator FixJ in the absence...
  14. Plasmid Cloning by Restriction Enzyme Digest (with Protocols)

    Type
    Protocol
    ...restriction sites to flank your insert : You can use PCR Based Cloning and add restriction sites to the ends...as long as it is already bounded by restriction sites that are also present in the same orientation on...Analyzer , allow you to identify which restriction sites are present in a given sequence. When selecting ...recipient plasmid as well as a specifically designed test digest later to verify that the insert was cloned...a version of your insert flanked by restriction sites compatible with the recipient plasmid's MCS. However... within your insert. Adding desired restriction sites to your recipient plasmid : You can modify the MCS...
Showing: 701 - 720 of 957 results