... and have worked closely with us to functionally test our virus. Ed Boyden’s lab from MIT and Alla Karpova... methods, performs extensive quality control, generates  educational materials, and provides viral vector...

...parts: the receptor itself and the G-proteins it associates with.  Receptor: GPCRs are also known as seven...transducing receptors in the brain they are ideal candidates for use in chemogenetics. To create chemogenetics...

...  Fig. 1: Schematic overview of ChIP. Image courtesy of Shengliu via Wikicommons.   Okay, let’s...celebrating a successful ChIP experiment! Image courtesy of https://www.wannapik.com/   Even though...

...2: comparison of Cas 9 vs. Cas 12a expression cassettes when encoding multiple guides. While two guides... and fusion proteins, such as Cas9 and Cas12a, creates challenges for in vivo delivery and cell engineering...

...security-critical bugs, and contribute to constant updates of the software. In hardware, this process is often...are as follows: Detailed documentation, which facilitates the reproduction of the structure in a similar...

...small molecule realm is typically not feasible to test in any given screening experiment. Thus, a limited...require there to be a culture condition which eliminates manipulations that do not affect the function...

...activate all 20,000 genes, it is not possible to test each one and make sure it’s working. You can only...resistant to treatment with a BRAF inhibitor. (The test was the perfect complement for one conducted earlier...

...restriction sites to flank your insert : You can use PCR Based Cloning and add restriction sites to the ends...as long as it is already bounded by restriction sites that are also present in the same orientation on...Analyzer , allow you to identify which restriction sites are present in a given sequence. When selecting ...recipient plasmid as well as a specifically designed test digest later to verify that the insert was cloned...a version of your insert flanked by restriction sites compatible with the recipient plasmid's MCS. However... within your insert. Adding desired restriction sites to your recipient plasmid : You can modify the MCS...

...theaters, the Razr was the hottest phone on the market, and two new websites, Facebook and Gmail, were ...

...communication journey started with something unexpected: termites. “I was really interested in the evolution of ... science writing. She decided to start a blog to test it out and discovered she not only loved science...

...(Japanese, German, and English) and even on 60 Minutes. We are blessed with a large community of scientists... employee pool and we get a lot of successful candidates from internal referrals. Our long reaching reputation...

...as KLH or Freund’s adjuvant is injected, which stimulates the overall immune response. Once the initial...weeks to increase the antibody titer. The titer is tested following each immunization until the desired level...

...of the gold particles were in the cytoplasm of dendrites, and only a small proportion were found on the... HA tag (bottom). The images show examples of dendrites (blue shades) in the monkey amygdala. Red and ...

...and isolated with antibiotic selection. The team tested this system in vitro and in vivo. They identified...fluorescent proteins into one bright particle that segregates into one cell during cell division. To achieve...

...load The Wikipedia definition of cognitive load states: “cognitive load refers to the used amount of working...even the most enthusiastic of learners.  What contributes to cognitive load?  Everything! Here are some...

...idea of the basics of immunoimaging. A scientist conjugates an antibody with a signaling molecule, the antibody...secondary antibodies on hand and use them to easily test different antibodies or different signaling molecules...

...express genes or gene sets over 4.7 kb. Co-infection rates for AAVs are generally high enough for this approach... guest blogger Didem Goz Ayturk with edits and updates from Addgenies Karen Guerin and Susanna Stroik....

...reagents and consumables, especially for things like plates and gels, which either don’t scale or have limited...my ability to tell. But even from these rough estimates, it’s clear that community engagement with and...

...below): 1. Resistant cells among the separate replicates would contain the same labels - i.e. they would...subpopulation 2. Resistant cells among the separate replicates would have different labels - i.e. they would...

...engineering projects in a very big way. In one of his latest papers, published in Molecular Systems Biology ...that took a spaceship to the moon was built on NOR gates. It’s a fundamental unit. We did demonstrate that...