...is a STEM career counselor, Director of Education for the Jackson Laboratory for Genomic Medicine and a... successful and sustainable biomedical research sector at our first Future of Research symposium. The ...compulsory internship to be taken in an industry, regulatory, or clinical environment. It remains contested...the overall structure of the biomedical research sector, and what a tenable workforce structure may look...ideal and productive workforce look like for the sector – how many jobs are there for STEM graduates and...strong supporter of increasing the number of postdoctoral scientists at University of Maryland. Paula... at Scientific Meetings from Addgene Executive Director Joanne Kamens Learn how to be a successful manager...

... be viral vector jokes and comics. Other new resources for researchers using viral vectors In addition...Addgene blog Download our Viral Vectors 101 eBook Find all of our viral vectors blog posts Learn more about...Starting today, our Twitter feed will be full of viral vector information. Each day, we’ll cover a different ...AddgeneGoesViral to find information on the basics of viral vectors, applications, and some new resources available...also investigates new strategies to improve viral vector production and data analysis. Our first paper came...

...in viral vectors, but easily fit in a TE. Transposons are also less likely than viral vectors to induce...DNA transposons are most commonly used in the laboratory for genome manipulation. When transposons are...transposon’s LTRs, similar to when packaging viral vectors.   Let’s take a closer look at three different ... in human cells at rates similar to retroviral vectors. The hyperactive version of the SB transposase,...efficiency in human cells similar to retroviral vectors. The hyperactive PB transposase (hyPB) is ~10-fold...transfer Transposons are an alternative to viral vector delivery of transgenes, like for iPSC reprogramming... piggyBac, which is a big advantage over viral vectors (~5 kB payload for AAV and ~8 kB payload for lentivirus...

...Hendel et al. 2015). Low detection rates mean the investigator can be relatively certain that their samples...off-target mutations are essential data points for investigators looking to definitively link genotype and phenotype...Targeted amplicon sequencing doesn’t tell the whole story Figure 1: Schematic representations of the...viable options for multiplex sequencing in many laboratories. Nevertheless, unbiased detection isn’t as sensitive...genome-wide unbiased methods. However, most investigators use single cell clones for in vitro CRISPR experiments... Hough. We additionally thank Monica Sentmanat, Victor Dillard, and Ayokunmi Ajetunmobi for their contributions... Hough is a Biochemistry PhD candidate in the laboratory of Prof Steve Jackson at the Gurdon Institute...

...nick, and monitor indel frequencies (Anzalone et al., 2019). Figure 6: Prime editors bound to ... Figure 4: Editing the PAM prevents the prime editor from re-engaging with DNA it has already edited...to pegRNAs drive editing (left), while prime editors bound to sgRNAs only drive nicking (right). Start ...opposite strand. Created with BioRender.com. MMR inhibitors PE4 and PE5: These approaches inhibit MMR to...inhibited. Created with BioRender.com. RNA protectors epegRNAs: epegRNAs include structured motifs...primer binding site (Nelson et al., 2022). PE7: This editor includes the fused RNA-binding protein La to protect...software, however. You may find it useful to ask a mentor what software tools they recommend — feel free ...

...cells survive their own secretions. Pro tip! Historically, colicin-producing plasmids have been referred...referred to as “Col plasmids” or “Col factors.” Virulence plasmids: microbial infection Virulence plasmids...other molecules to host organisms. For instance, vectors derived from the Agrobacterium Ti virulence plasmid... through direct contact. That’s conjugation! F factors can also be integrated into the bacterial chromosome...developing new cloning techniques based on these vectors (Shareck et al., 2004). Plasmid categorization...the many plasmid tools available in the Addgene repository. To learn more about some other common plasmid...Auriol, P., Vogel, T. M., & Simonet, P. (2001). Laboratory-scale evidence for lightning-mediated gene transfer...

...Washington Office Ben Corb - Director of Public Affairs at ASBMB Adam Fagen, PhD - Director, Early Career Scientist..., these issues which will continue to shape the sector throughout their careers (McDowell et al, F1000Research...working group (National Academy of Sciences. “The Postdoctoral Experience Revisited” 2014 Appendix B p93-95...funding and funding mechanisms require an engaged sector with effective advocacy skills. Advocating for ...Scientist Segment at AAAS Marnie Gelbart, PhD - Director of Programs at Personal Genetics Education Project...their respective fields: David Cameron, PhD - Director of Media Communications, Harvard University Ray...Advocate with the Perfect Science Talk Find a Good Mentor ...

...Diagnostics will play an important role in helping to monitor and slow this spread until vaccine programs can... switches for pathogen detection In 2014, as postdoctoral fellows with Jim Collins and Peng Yin at the.... The first was a new class of programmable riboregulators, called toehold switches, which allow for the...allowed gene circuits to be deployed out of the laboratory in a cell-free and biosafe format that was stable...circuit-based sensors to be used outside of the laboratory. The detection threshold for these RNA sequences...the technology through product development, the regulatory process, and manufacturing so that we can get...Leslie Dan Faculty of Pharmacy at the University of Toronto. His research is focused on developing cell-free...

...deliver in low-capacity vectors like AAV. AAV (adeno-associated viral vectors) are commonly used for in...Background Read About CRISPR Competitor NgAgo Learn How You Can Use piggyBac Vectors in Genome Editing Resources...preferential infection of certain tissues. However, these vectors have a low genetic capacity of ~4.5 kb. At 4.1 ...barely fits into AAV when you add in a gRNA and regulatory elements. To solve this problem, the Zhang lab...previously used SaCas9 (1053 aa/3.2 kb) with AAV vectors for efficient postnatal editing. LbCpf1 and AsCpf1...SpCas9. These nucleases should fit well into AAV vectors, especially given that Cpf1 gRNAs are also shorter...

..., 50ms “off”.  A pulse generator such as the A.M.P.I Master-9 Pulse Stimulator, which can control output...cable is threaded through—and fixed to—an optical insulator called a ferrule (below). The fiber optic cable...Channelrhodopsin 2 (Chr2) has been expressed in the motor cortex of a mouse. Upon connection of the ferrule...to give the animal the full range of motion, a commutator allows the patch cable to connect to the laser...University of Michigan and he is currently a Postdoctoral Fellow in the lab of Mary Jeanne Kreek at the... 1. Gutierrez DV, et al. Optogenetic control of motor coordination by Gi/o protein-coupled vertebrate ... neurons. Current protocols in neuroscience / editorial board, Jacqueline N Crawley  [et al]. 2012;Chapter...