We narrowed to 1,073 results for: TOR;

...exactly how postdoctoral salaries are changing. Transparent salary information for postdoctoral positions...institutions set their postdoctoral salary scales to the NRSA scale (Figure 21, National Postdoctoral Association...was contributed by Future of Research Executive Director, Gary McDowell. On Dec 1st, the threshold at which...when the ruling was issued, a joint blog post by Director of the NIH Francis Collins and Secretary of Labor...paid. Institutional salary policies are one indicatator of how salaries may changes due to the FLSA. ...as having postdocs, representing ~85% of the postdoctoral workforce. Based on our communications with ...according to the NSF data, account for 18% of the postdoctoral workforce in the sciences. The Dana Farber Cancer...

...the activator - the CRY2 photolyase homology region (CRY2PHR) fused to a transcriptional activator domain...directed by the guide RNA (gRNA), while the CRY2PHR-activator fusion floats freely, depicted in the figure below...and CIB1 proteins heterodimerize and move the activator into position to activate gene transcription. ...manner. With their light-activated CRISPR/Cas9 effector (LACE) system, the Gersbach lab utilized a similar...fusion of full-length CRY2 and the transcriptional activator domain VP64. Using this system in HEK293T cells...photoswitchable proteins derived from the fungal photoreceptor, Vivid (VVD, N. crassa) (Kawano et al., 2015...transcription, they fused BldD to the transcriptional activators p65 and VP64 which in turn transcribes N-Cas9...

... and progenitor cells. Now with the DAP array, it is possible to use the state-of-art base editor, prime...prime editor, or any CRISPR-based gene editors, for correcting mutations or installing functional genes...thought to enable MBE by using Cas12a fused base editors (dCas12a-BE). However, the poor editing efficiencies...they found led them back towards Cas9 fused base editors (nCas9-BE), as Cas9 is mechanistically more favored...number of gRNAs that can be packaged into viral vectors for potential in vivo applications or multiplex...array.7 Using DAP arrays with a variety of base editors, the Gao Lab successfully demonstrated up to 31...unprecedented number of genes with a single viral vector.   Fig. 1: Schematic of multiplex gene ...

...adjacent fragment or vector. 5′ overhangs are generated on all the fragments and the vector, specifying the... together through transformation, so the cloned vector must be ligated. But in LIC, longer single-stranded...due to the absence of the other dNTPs. Once the vector and insert are (separately) digested to create ...received an important update, courtesy of Addgene depositor Stephen Elledge. His new method, named sequence...homologous recombination. SLIC inserts and linearized vectors must have homologous DNA sequences (20–60 bp) on...complete plasmid. Besides being compatible with any vector, the SLIC protocol itself is flexible. For one ...digestion (no dNTPs needed), allows both insert and vector to be digested in the same tube. Alternatively,...

...calcium indicator (Shemetov et al., 2021). In addition to FRET, intensiometric calcium indicators NIR-GECOs...proteins (NIR FPs) were derived from natural photoreceptors incorporating biliverdin IXα (BV) autocatalytically... derived from bacterial phytochrome (BphPs) photoreceptors have higher apparent cellular brightness than...were derived from cyanobacteriochrome (CBCR) photoreceptors (Oliinyk et al., 2019). They were engineered...performance, and applications of near-infrared calcium indicators in this recent review (Shcherbakova et al., 2021...encoded near-infrared fluorescent calcium ion indicator. Nat Methods 16, 171-174 (2019). Matlashov, M..... A near-infrared genetically encoded calcium indicator for in vivo imaging. Nat Biotechnol 39, 368-377...

...genes. We’ve created enough regulatory elements, CRISPRs, repressors, activators. We’ve been putting together...CRISPR/Cas genetic circuits are linked to the regulatory networks within E. coli host cells. ...in building genetic circuits is getting enough regulatory proteins that don’t interfere with each other...programmable that you can make a very large number of regulators. That basically means larger genetic circuits...that could conceivably be as large as natural regulatory networks. Addgene: What kinds of things could... pattern, shape. All of that is controlled by regulatory networks in the plant. If we want to access that...CRISPR is how much easier it is to work with than regulatory proteins. There are still things we haven’t solved...

...massive amount of information regarding which regulatory proteins, signaling molecules, mutations, and...little is known about the order or timing of these factors. Recombinase-based state machines (RSMs), which...In the absence of extra recombinase-specific co-factors (which should be excluded from the system), these...paper (1). Lastly, it is possible to build gene regulatory RSMs (GRSMs, the subset of RSMs that regulate...also demonstrates that genetic parts (genes, terminators, and promoters) may be interweaved onto a GRSM...parts used in this paper (recognition sites, terminators, promoters, and genes) were built with flanking...them. We recommend using strong, directional terminators and promoters with buffer sequences on their ...

...Mice can be engineered by inserting a targeting vector containing the gene of interest into a zygote, ...microinjection of DNA or infection with a viral vector containing the gene construct can be used to insert...generated via homologous recombination. A targeting vector is electroporated into embryonic stem cells and...engineer knockin mice. Co-injection of a targeting vector containing homologous ends to the breakpoint promotes...promotes homologous recombination of the targeting vector. CRISPR/Cas9-based systems have been developed ...the expression of a particular gene. Targeting vectors used to create knockout mice include a reporter...standing technique is the injection of a targeting vector into embryonic stem cells, which are then injected...

...Looking for a mentor or want to start a peer mentoring group? "What Makes a Good Mentor?" Form Your Own...form a peer mentoring group to get real feedback Write whenever you can, find supportive editors so your ... Own Peer Mentoring Group: A How-To Guide for Scientists Download our eBook: Mentoring for Scientists ...your PhD or perhaps you have almost completed a postdoctoral position… or two. You have learned a lot. Whether... scientists, create and stick to timelines Be a mentor in a formal program Start something (a journal,... plan a conference Recruit many supporters and mentors that will speak for you (and you for them) Communication...

...: A knockout targeting vector designed to insert a resistance gene. The vector contains a neomycin resistance... homologous sequence using a specially designed vector that contains sequence homology to the locus of... of the targeted gene have been cloned into the vector on either side of an antibiotic resistance gene...Figure 2). Figure 2: A knockout targeting vector designed to insert and later remove a resistance... the sequence to be inserted is cloned into the vector between the homologous sequences together with ... Figure 3: A knockout/knock-in targeting vector designed to insert GFP into a given locus. ...earlier. In this case, you design your targeting vector such that a set of three LoxP sites flank the resistance...