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We narrowed to 971 results for: lars;

Showing: 361 - 370 of 971 results

  1. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type
    Blog Post
    ...potently inhibit type II CRISPR systems including the popular S. pyogenes Cas9 (Rauch et al., 2017, Hynes et ... on a single plasmid (Grefen and Blatt, 2012). Similar to traditional Gateway™ cloning, the GOI first ... (FRET, Heckler et al., 2015) or ratiometric Bimolecular Fluorescence Complementation (rBiFC, Grefen and...offers a way to spatially and temporally control cellular processes in vivo. Many photoactive proteins exist...photo-reversible protein-protein interactions can control subcellular localization processes in vivo. They deposited...the mitochondria, because cPYP binds to BoPD. A similar set of newly identified light- and dark- binder...

  2. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    ...can be particularly useful in academic labs when creating shared experimental protocols. Clarify: Asking...setting, where student and postdoc turnover happens regularly and it behooves everyone in the lab to be using...Asking for clarification during a meeting can help get everyone on the same page. Others in the meeting might...communication has helped Addgene move from completing 2-3 large projects a year, on average, to completing 8-10 ...understanding you can be incredibly frustrating. Clarifying the words —> decreasing frustration —> increasing...

  3. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    ...: IL-36 biology in generalized pustular psoriasis Generalized pustular psoriasis is a chronic autoinflammatory...in this educational video. “SLC6A8 belongs to a large family of solute channels that are responsible for...outside of the cell and translate those signals into cellular responses. For example, GPCRs and their downstream...different receptors upon stimulation. “To study cellular transduction events, we will utilize BRET-, FRET...intensity-based signaling biosensors to monitor intracellular signaling,” says Hébert. Previous research has...

  4. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...time intensive to process a large number of microbiome samples. To enable large-scale, longitudinal studies...Gram-negative cells.  The Wang lab further developed a modular suite of plasmids (pGT) with various replicative... honey bee microbiome. The toolkit consists of modular parts that are assembled with Golden Gate Assembly...other microbiome systems. Find the toolkit for modular assembly of broad host range plasmids Counting ...bee gut microbiome bacteria with a toolkit for modular assembly of broad-host-range plasmids." ACS synthetic...

  5. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ...reagents.  Affinity Reagents  Affinity reagents are molecular tools that have the ability to specifically recognize...affinity reagents is their size. Antibodies are large enough that their bulk can be a barrier at times...difficult to source, may not be readily available for a large number of targets, and can require specific conjugates...   Types of Affinity Reagents  (Listed in no particular order.) Fab fragments are created through enzymatic...variables (scFvs) are ~27kD engineered fusion proteins similar to a Fab fragment but created through engineering...eukaryotic cells, allowing for them to be used as intracellular inhibitors.  Aptamers are not proteins at all...

  6. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    ...encode proteins. They play important roles in many cellular and regulatory processes, but much of their functionality...removing the full ncRNA locus (Ho et al. 2012). For large-scale screening, sgRNA libraries are useful for ... same time (Liu et al. 2018). However, removing large lncRNA fragments with multiple gRNAs poses the risk...Zibitt et al. 2021). Mutating splice sites is particularly effective as it can result in the retention ...Mutations of splice sites. (C) Removal of an exon or a large genomic fragment. (D) Knock-in: insertion of a synthetic...used to target lncRNAs with high specificity, particularly in applications requiring smaller delivery systems...

  7. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    ...developed third option: Fragmid. Fragmid is a modular vector assembly process designed for a quick, plug-and-play...vectors in five days. Did you say… easier? Fragmid’s modular assembly structure takes a lot of the design work... And it truly is plug-and-play. Because of its modular cloning approach, it’s easy to switch out reporters... by phiC31   The Fragmid website While the modular cloning approach is key to the Fragmid system, ..., M. A., Green, T. M., & Doench, J. G. (2024). Modular vector assembly enables rapid assessment of emerging....2024.100519 More resources from Addgene Plasmids 101: Modular Cloning Addgene's CRISPR Guide Plasmids 101: Golden...

  8. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

    Type
    Blog Post
    ... to interact by stimulation with blue light.  Similarly, light can also be used to control homo-oligomerization...distribution of clusters without accumulation in subcellular compartments such as nuclear speckles. Furthermore...OptoSTIM1), caused two-fold faster changes in intracellular Ca2+ levels than the original version containing...CRY2clust should prove a useful tool to further probe cellular function. The CRY2clust Plasmid can be found here... commonly observed with direct stimulation are largely absent in the soCoChR-expressing system. Taken ...interactions. We’re excited to see how you adapt similar FLINC biosensors to work in your lab. Find the ...

  9. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

    Type
    Blog Post
    ...and TadA7.10, they designed one set of ABE8’s similarly (ABE8.x-d). In order to assess the effect of removing...you need to modify multiple genes to generate a cellular phenotype. Again ABE8.20-m was the winner. It ...editing in ABE8s While base editors are precise molecular tools, they do have undesired off-target editing...have been described to introduce base changes in cellular DNA and RNA dependent or independent of the gRNA..., and a thoughtful selection of guideRNAs. Of particular note, ABE8s reported in this work did not cause...sites that used to be difficult to target. In particular, their ability for more robust editing in human...

  10. Synthetic promoter AAVs for cell-type specific expression in retinal cells

    Type
    Blog Post
    ...genes in particular tissues, but they are costly and time-consuming to generate, especially for large animals...Adeno-associated virus (AAV) is a popular tool for gene delivery, but it has a drawback: how do you ensure... it to? Knowing that a gene is expressed in a particular cell type is important not only for translational...types.  Botond Roska’s lab at the Institute of Molecular and Clinical Ophthalmology Basel recently took...Besides restricting AAV transgene expression to particular cell types or classes, these synthetic promoters...
Showing: 361 - 370 of 971 results