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...ve ever wondered how they are different from or similar to the other Ts, we have your answers. If you know...into a cell by a viral agent. Transductions are similar to transfections, processes which introduce foreign...or non-integrating. Read on to find out more! Molecular basis of transduction Transductions utilize the...transduction step. Viral particles are secreted into the cellular media, making viral collection fairly easy. Producer...available for a variety of broadly applicable and popular viral vectors. Check out our viral selections to... Transfection and transduction seem to achieve similar outcomes, so why and when should you opt to make...

...a true measurement of high affinity (i.e. sub-nanomolar) protein-ligand interactions. Avidin and its derivatives...to a lysine residue within the AviTag. However, large amounts of the target protein are typically required... protein (MBP, New England BioLabs). MBP has a similar function as GST in that it provides a method to...successfully used this plasmid to biotinylate the extracellular domain of the erythropoietin receptor and subsequently...biochemist in the Vanderbilt Eye Institute and is particularly interested in cell signaling.     Additional...Technologies Resources at Addgene.org Learn about Popular Plasmids in the Repository Learn about New Plasmid...

...Repeating sequences within a plasmid can undergo intramolecular DNA recombination. This naturally occurring...recommendations from the depositing lab. If your vector is large (>10 kb) or has a low copy number, try following...agar plates, you may notice that there are both large and small colonies. Bacteria containing the recombined...plasmid, whereas the round tan colonies that look similar to normal E. coli cultures contain the recombined...: electron microscopic analysis of in vitro intramolecular recombination. Proceedings of the National ...Additional resources on the Addgene blog Find more molecular biology and viral vector protocols Learn about...

...Now, scientists are beginning to see Addgene as a large data set. Addgene has over 65,000 plasmids in the... in synthetic biology. “Synthetic biology, in particular, the ability to do DNA synthesis, is evolving...protein, their gene sequence may have slight or large variations depending on how closely related these...within the Addgene repository is mammalian, but the largest source of unique gene sequences is Proteobacteria...the only time researchers have used Addgene as a large data set. Alec A. K. Nielsen & Christopher A. Voigt... page Search for a plasmid Find Addgene's most popular plasmids ...

...Stability improvement of antibodies for extracellular and intracellular applications: CDR grafting to stable...image nascent proteins. In addition GFP tags are large, dim, and permanently attached to a protein, making... detects the widely used HA epitope in diverse cellular environments. They called this probe the HA frankenbody...else can you do with the HA frankenbody? Another large advantage of the HA frankenbody is that it remains... fluorescent protein guide Find plasmids for subcellular localization Find fluorescent protein plasmids...

...monomeric variants of Clavularia cyan fluorescent protein: identification of the molecular determinants of ...tetrameric cyan fluorescent protein from the coral Clavularia sp. protein CFP484. Hui-wang Ai and colleagues...nm), making it useful in multicolor labeling particularly with fluorophores that excite in the violet ...expected locations. An analysis of mWasabi gave similar results. We hope this blog post helped you see ... monomeric, bright and photostable version of Clavularia cyan fluorescent protein: structural characterization...

...accumulates in the endoplasmic reticulum, but exhibits larger fluorescent responses to GABA. Find the iGABASnFR... visualize cells and to quantify targeting of particular cell types. For example, the mDlx enhancer restricts...for experiments encoding a fluorescent-tagged molecular tool.   Find pAAV-FLEX-tdTomato, pAAV-GFAP104-...on availability date or to be notified when a particular catalog item is available for order please email...fluorescence. It is useful for activity imaging of large populations of densely labeled neurons because background...fluorescence.  It is useful for activity imaging of large populations of densely-labeled neurons because background...

...unruly. Instead of settling down into a singular focus on molecular pathways, I found myself curious about... a look at the blog ‘Scholarly Kitchen’ for a glimpse into the world of scholarly publishing. And see ...programs unfold, absorbing the stochasticity of molecular interactions and embedding individual diversity...viewed through the lens of development, cell, and molecular biology. Beyond that, she is also a fan of baseball...

...eukaryotic cell for the execution of many complex cellular behaviors, such as differentiation or metabolism...metabolism. Our attempts to manipulate these cellular behaviors often fall short with the generation of various...control, a new study repurposes the sgRNA to include modular synthetic RNA scaffolds that can recruit various...master ON/OFF switch. This will likely prove particularly useful in avoiding toxic intermediate buildup...technique that it should be on the radar of every molecular microbiologist. Summary  The single guide RNA...the CRISPRi system can be repurposed to include modular RNA scaffolds. These RNA scaffolds can recruit...

..., which can be done by isolating animals with particular visible phenotypes. These approaches may become...) with 30-60 basepair homology arms to knock in large epitopes, such as GFP (Paix et al., 2014). A further...my initial direct screening efforts, which were similar to the approach detailed by Paix et al. Although...Addgene. In 2019, Farboud et al. described another similar co-conversion marker (repair of a zen-4(cle10ts...facilitated CRISPR editing in C. elegans. These modular toolkits build single plasmid targeting vectors...the “elegans” methods outlined in this post – particularly the use of co-selection methods – could greatly...Tzur YB, Friedland AE, Nadarajan S, Church GM, Calarco JA, Colaiácovo MP (2013) Heritable Custom Genomic...