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We narrowed to 955 results for: ARC;

Showing: 721 - 740 of 955 results

  1. Design Tips for Prime Editing

    Type
    Blog Post
    ...! First, a quick refresher: prime editing is a “search and replace” genome editing technique that relies...Newby, G. A., Raguram, A., & Liu, D. R. (2019). Search-and-replace genome editing without double-strand...

  3. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ...switches were designed as a genetic tool for researchers to conditionally manipulate gene expression in...allowing for expression of GeneR. This allows a researcher to test whether or not expressing GeneR alone...

  5. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    ...FRET based tools to study epigenetics Epigenetic research uses a wide range of molecular biologic techniques...none of these techniques can be used for in-situ research of dynamic modifications in real-time, posing ...

  6. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    ... the battle when using optogentics to answer a research question. The flip side of the optogenetics coin... molecules in freely moving mice. Neuroscience research. 2011;70(1):124-7. doi: 10.1016/j.neures.2011.01.007...

  8. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ...bloggers Alissa Lance-Byrne and Alex Chavez, researchers at the Wyss Institute for Biologically Inspired...University of California, Santa Cruz, where her research is focused on engineering nanoparticles as drug...

  9. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ...and could be contaminated.Learn how Addgene uses barcodes to track samples Keep track of passage numbers... Addgene.org Find mammalian plasmids for your research Find viral vectors for infecting mammalian cells...

  10. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    ... A recent survey of PhDs found that many researchers feel that they lack formal training in a variety...out to fill this gap by both highlighting that researchers do learn MANY transferable skills while working...

  11. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ... of a gene exists, there must be assays that researchers can use to quantify said copies. They do so using...commonly available and may not be an option to all researchers. Accuracy needed Do you need to call exact...

  12. Transferable Skills: Negotiation

    Type
    Blog Post
    ...A 2017 survey found that many researchers feel they lack formal training in a variety of transferable...set out to fill this gap by highlighting that researchers learn many transferable skills while working ...

  13. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    ...recent studies (Mathis et al., 2024) and concurrent research by the Shendure lab (Li et al., 2024) have highlighted...Newby, G. A., Raguram, A., & Liu, D. R. (2019). Search-and-replace genome editing without double-strand...

  14. Antibodies 101: Flow Compensation

    Type
    Blog Post
    ...has a research background in CD8+ T cells and cancer immunotherapies. She is currently a research technician...

  15. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ...of CRISPR-based immune systems in bacteria and archaea. Class 1 is further broken down into different ...ubiquitous in nature, they are largely underutilized in research settings — mostly a consequence of Cascade's complexity...

  16. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...from one serotype and the capsid of another. Researchers have used these psuedotypes to further improve...-022-00700-y Hanlon, K. S., Kleinstiver, B. P., Garcia, S. P., Zaborowski, M. P., Volak, A., Spirig, S...

  17. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    ...resistance gene can affect a cell's phenotype, researchers usually remove it after selection using the Cre...conditional knockout. To make a conditional knockout, researchers often use the Cre/Lox system described earlier...

  18. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ...This post was updated on March 21, 2018. Most of the time, plasmid prepping is a breeze. You get your...copy number of the empty plasmid backbone or a researcher’s personal observations. If you are experiencing...

  19. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...If you follow CRISPR research, you know all about using non-homologous end-joining (NHEJ) to make deletions...al.’s use of MMEJ represents another strategy researchers can exploit for CRISPR gene editing. In organisms...
Showing: 721 - 740 of 955 results