We narrowed to 730 results for: ACE
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TypeProtocol...and transduce Cells Day 2–3 (am): Remove media, replace with fresh media containing selection reagent Day...important to monitor these cells regularly and replace the cell media. Cell death by some cells in the...
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Plasmid Cloning by PCR (with Protocols)
TypeProtocol...versatile and allows for nearly any piece of DNA to be placed into a backbone vector of choice with minimal limitations... important to have nice crisp bands and to have space to cut out the bands. Because of this we recommend... -
Coomassie Purity Stain of Recombinant Antibodies
TypeProtocol...remove the white sticker on the bottom of the gel. Place the gel in the electrophoresis chamber and secure...where dye is visible. Section 2: Staining the Gel Place the gel in a plastic tray with 100 mL of deionized... -
DNA Quantification
TypeProtocol...solvent. If using a NanoDrop to measure your samples, place 1-2µL of mini-prepped DNA onto the pedestal. Close... -
Protocol - How to Create a Bacterial Glycerol Stock
TypeProtocol...lid and the tube of a glycerol stock before you place the sample at -80°C. Frozen tubes are hard to write... -
Protocol - How to Streak a Plate
TypeProtocol...that you can make a broad stroke. Only touch the surface of the plate, do NOT dig into the agar. Another... -
Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps
TypeProtocol...choice AAV Purification by Iodixanol Gradient Ultracentrifugation Purify adeno-associated virus from a preparation... -
Molecular Biology Protocol - Restriction Digest of Plasmid DNA
TypeProtocol...electrophoresis . Tips and FAQ Restriction enzymes MUST be placed in an ice bucket immediately after removal from... -
Plasmid Cloning by Restriction Enzyme Digest (with Protocols)
TypeProtocol... important to have nice crisp bands and to have space to cut out the bands. Because of this we recommend... -
Isolating a Monoclonal Cell Population by Limiting Dilution
TypeProtocol...well plate. Allow the cells to grow overnight. Replace the medium on the cells and collect the used (conditioned...