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  1. All in a Twist: dsRNA

    Type
    Blog Post
    ...power of dsRNA for a long time, even before we knew about all the diverse in vitro production mechanisms. ...repetitive sequence. All of the R-loops described above play distinct roles in promoting cellular processes...

  2. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    ...they get old, they lose competency). If all the above is correct, you should probably have a second and...blog posts Read blog posts on plasmid cloning Read about different plasmid elements Resources on Addgene.org...

  6. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    ...in modern cloning, and why should you learn more about it? Read on to find out how ccdB can make your cloning...of this system for enhancing cloning efficiency about 20 years ago and developed cloning vectors to harness...

  7. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...discussed below. How does it work? As alluded to above, the Cre recombinase catalyzes the site specific...Combining this with some of the loxP methods described above, a genetic modification can be restricted based ...

  8. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    ...Destination vector (dropdown options depending on above selections) Module set (the set of components you...vectors) Target type (drop down options depending on above selections.) CRISPR enzyme (Cas9 [sp], Cas9 [other...

  9. CRISPR in the Clinic

    Type
    Blog Post
    ...trials The CRISPR therapies don’t stop with the list above! There are many, many more in the earlier stages... and others we just don’t have the time to talk about. Other treatments currently in clinical trials include...

  10. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    ...cycling. The Pros and Cons Researchers used the tools above to complete a 52-piece assembly of a 40 kb T7 phage... help from DAD. The 35-piece assembly mentioned above had a remarkably high 71% fidelity, while the 52...

  11. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    ...blog Find our CRISPR blog posts Read blog posts about using Cre-lox Read our genome engineering blog posts... posts Resources on Addgene.org Find more about Cre-lox Read our Genome Engineering Guide Learn more...

  12. Hot Plasmids - October 2020

    Type
    Blog Post
    ... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here.  Here's...fluorescent proteins coupled with Fibronectin intrabodies generated with mRNA display (FingRs), Xue Han...

  13. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    ...Bondy-Denomy et al., 2018). For example, AcrIIA4 mentioned above indicates that it’s the fourth Acr protein discovered...Cas9 Variant for Your Next Experiment Read more about Optogenetic Control of CRISPR   Resources on Addgene.org...

  14. Progress Towards a PAM-Free CRISPR

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    Blog Post
    ...experiments, therefore, you often must make a choice about what is your top priority: location or efficiency...These Cas proteins have everything we know and love about Cas9, but with broader PAM compatibility. This alternative...

  15. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...s chromatin status into account (Fig 1a). The laboratory of Karmella Haynes at Emory recently contributed...custom design their own engineering plasmid tools in about a week. Taking advantage of Golden Gate cloning,...

  16. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    ...available systems. Producing lentivirus: it's all about the T's (transfection and titers) To produce lentivirus... optimize transfection conditions. As mentioned above, plasmid ratios can be adjusted to enhance titer...

  17. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...connected to the neck. Shortly after, we learned about organs and the jobs they perform. This became foundational...part of the answer to many biological questions about the brain. To address this, molecular tools to map...
Showing: 721 - 740 of 963 results