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We narrowed to 964 results for: App;

Showing: 721 - 740 of 964 results

  2. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...of LSFM microscopes (3,4). After this initial application, Stelzer’s group described the single-plane or...shadows along the illumination path. The shadows appear as stripes in the acquired image (5).  A way to...

  4. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...emission is green or red light. For example, T-Sapphire, LSSmOrange, and LSSmKate. Fluorescent Sensors.... al. (2010) “Fluorescent proteins and their applications in imaging living cells and tissues”  Physiol...

  5. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    ...and retroviral expression system for using this approach in in vivo studies (Fujita et al., 2018). In addition...DUX4, a transcriptional regulator that when inappropriately expressed can cause facioscapulohumeral muscular...

  6. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...101: Types of Viruses Viral Vectors 101: Viral Applications Resources on addgene.org Addgene's Lentiviral...Liu, H., Xiao, H., Conway, J. A., Hunter, E., & Kappes, J. C. (1997). Functional RT and IN incorporated...

  9. Protocol - How to Streak a Plate

    Type
    Protocol
    ...(with appropriate antibiotic) Bacterial stab Procedure Obtain an LB agar plate with appropriate antibiotic...working near a flame or bunsen burner. Obtain the approrpriate bacterial stab or glycerol stock . Using a sterile...

  10. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...fluorescent proteins (FP) have also been important in mapping, monitoring, and manipulating neuronal circuits...combining advantages of FPs and epitope tags, smFPs appear to be very effective probes for finely visualising...

  11. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...workflow - analyzing the data - is often under-appreciated or over-looked.  Synthego has developed a new...analysis Once the analysis is complete, a new screen appears with a graphical representation of the results ...

  15. How to Write a Scientific Review Article

    Type
    Blog Post
    ...papers and then feel ready to write. What actually happened was that each paper taught me a few things and...to read every single paper. I also learned to appreciate the discussion sections of the primary literature...

  16. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ...sequences into multiple vector systems. With the appropriate entry and destination vectors, one can use Gateway...elements to express the gene of interest in the appropriate system Recombines with the entry clone to generate...

  17. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ...from random mutations in the genetic sequence that happen to be beneficial, and persist as a result. Why ...are susceptible to the drug, while the few that happen to possess resistance genes survive and multiply...

  18. Hot Plasmids - August 2020

    Type
    Blog Post
    ... have created a yeast-based directed evolution approach with some notable improvements. Directed evolution...specific guide RNA (gRNA) sequences. But these approaches rely on continual expression of both dCas9 and...

  19. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    ...Motif (PAM), the sequence NGG.  If these conditions apply to your experimental target, SpCas9 and its enhanced...specificity over a single SpCas9. The drawback of this approach is that two sgRNAs targeting opposite strands ...
Showing: 721 - 740 of 964 results