We narrowed to 1,071 results for: CAN

... promoters, has limited the number of gRNAs that can be packaged into viral vectors for potential in vivo...the Gao Lab demonstrated that the tRNA-gRNA array can drive the expression of itself, without using any...gene editing with DAP array   Use DAP arrays can enable efficient multiplex gene editing, such as ...different tRNAs with optimized 5’ leader sequences can be used together in DAP array. To construct DAP array...their initial testing. It outperformed other tRNA candidates (either from human or plant sources) in both ...

...a variety of roles in synaptic communication and can trigger other forms of neuronal signaling and regulation... The yellow (v82) and lime green (v857) versions can be summarized quickly as: iGluSnFR3 v82: increased...being deposited at Addgene. Other plasmids and pAAVs can be ordered directly from Addgene. The Podgorski Lab...provided a brief breakdown of the promoter systems you can use to express the iGluSnFR3 variants: pRSET: Bacterial...

...space per user, but, if you need more space, you can sign up for a plan. Best of all, by not using paper...also a very helpful aspect to this app; the user can search by sequence, category of enzyme, or name to..., and structures into one convenient resource. I can safely say that I still have the single and triple...codon list for each amino acid, but, overall AMINO can be beneficial for new researchers or students beginning...your notebook. The files uploaded are saved and cannot be deleted, so there is no worry about misplacing...find an MSDS for a lab chemical of choice. Now, I can’t guarantee every reagent in your chemical closet ...quizzed randomly about a hazardous chemical and can’t find your lab’s MSDS book, this is a great resource...

...foods, and fibers. And because working with plants can be challenging, there are a lot of unexplored areas...not food, not medicine, and not mandatory – people can decide for themselves if they want this beautiful...body of knowledge. Organizations like Open Plant can then build on this research and effectively carry... pursuing scientific or business enterprises, he can be found climbing mountains or playing with his two...plant biology is a significant technical obstacle to plant synthetic biology. We cannot build what we do...now synonymous with GM to the point that people cannot imagine any use for GM technology other than to...sales. Flowers that change color throughout the day cannot be developed through conventional breeding; synthetic...

...manuscript needs to be revised before it can be published, this can delay publication and manuscripts may...that the manuscript requires revisions, the authors can publish a new version of the article, while the first...effort) and the scientific community, as the reports can be very informative as to how a paper is received...journals and their scientific communities aim to significantly improve the reproducibility and reliability ...

...suggestions. Can’t find a plasmid you’re looking for in the repository? Let us know! You can notify us to...plasmid and a publication where we can find the plasmid. While we cannot guarantee that everyone we reach...

...town, and other Cas proteins like SaCas9 and Cpf1 can circumvent the limitations associated with SpCas9...activation of the fluorophore. The fluorescent signal can be used as an indicator to determine whether the ...its structure and function? For starters, Cas13a can be used to bind and cleave target RNAs, although ...the exciting discovery that some Cas13a orthologs can function in mammalian and plant cells (Abudayyeh ...the protein in mammalian cells. LwaCas13a-msfGFP can mediate cleavage with spacers from 20-28 nucleotides...From this analysis, they identified 53 potential candidate genes that fell into 3 categories based on the...question: C2c1, C2c2 and C2c3 (short for Class 2, candidate 1, 2, or 3). C2c1 and C2c3 are related to Cpf1...

...channels. Since a Nextflow process can utilize any scripting language that can be executed on Linux, we created...computer, so it safely operates in isolation, and can be easily shared with others without any complicated...process defines which command or script will run and can be thought of as a particular step in the overall...step in our pipeline together.  Since each process can use a unique container, we customized each container...

...applications, and the quality of a standard curve can make or break an experiment. Here, we will provide... similar buffers if not, since buffer components can affect the final readout. When preparing a standard...user, equipment, assay incubation time, etc., which can vary even when the same protocol is being followed...sigmoidal. Once the trendline is calculated, you can extrapolate the concentration of the unknown sample...lowest and highest concentrations that the curve can accurately measure.  Dynamic range depends on a number...

...reusable software components that can be incorporated into software. You can think of these libraries as ingredients...educational, and professional backgrounds. Sometimes this can lead to wildly different ideas of how to approach... our software. What this means for us is that we can focus on the meat of the software and not waste time... places, we might be calling it an aliquot. This can lead to confusion as to what the code is supposed...development happening at Addgene under the hood. A significant portion of our development may not be visible...average of 40 minutes to 10 minutes. While users can’t tangibly see these improvements, you are benefited... been updated in several years) or they make significant changes that make it incompatible with our software...

...purified virus. This process can be completed in one long day, or the virus can be stored at 4 °C and buffer...separate into two phases. If this happens, the solution can be cooled and the two phases mixed together. Lastly...This approach gives me a bit of flexibility since I can store the cell lysates at -80 °C for up to 6 months...SYBR green technology or a TaqMan primer/probe set can be used and samples are quantified by comparing to...and Harshana de Silva Feelixge at Fred Hutchinson Cancer Center for teaching me about AAV production and...

... 2021) can help restrict expression to your cell type of interest, while miRNA target sites can be used...retrograde transport in neurons. (b) Genetic cargo can be customized to control transgene expression. Gene... cell type specificity. An on/off genetic switch can be used for expression control. (c) Viral delivery...which is one of a handful of natural serotypes that can cross the BBB. However, these natural serotypes (...Systemic capsids packaging Cre-dependent transgenes can also be paired with Cre-expressing transgenic mouse...spatiotemporal control of transgene expression or activity can be achieved using switches, including riboswitches...use two or more AAVs to transduce the same cell, can accommodate larger, modular genetic payloads. Since...

...fluorescent proteins, careers, and more. These resources can now be found in one place: the Educational Resources...Graphics by Beth Kenkel Find a round-up of tools you can use for making scientific graphics. PS: they’re free... transcriptase and a prime editing guide RNA, it can introduce insertions, deletions, and all possible...

...of the virus. While this can be useful, if too many neurons are labeled, it can be difficult to accurately...deficient (i.e. they cannot easily spread between cells like natural RABV or HSV can). AAV does not have...inserted electrodes) may be applied, but these methods can have a negative impact on cellular health. Another...Virus (HSV) also migrates through neurons where it can replicate and by doing so spread across several synaptic...immunogenicity and cellular toxicity. AAV vectors can be equipped with strong promoters for high and neuron-specific...fluoresce in different colors. An individual neuron can then be traced throughout the brain based on its ...Oh et al., 2014). Images yielded from this study can be found on the Allen Mouse Brain Connectivity Atlas...

...expresses the HIV gag, pol, rev, and tet genes. It can be used with 2nd or 3rd generation lentiviral vectors... proteins determine which types of cells a virus can infect. The VSV-G envelope protein enables this virus...BbsI restriction site, a pair of annealed oligos can be cloned to create a chimeric guide RNA. Find pX330...producing lentiviral and MuLV retroviral particles. It can be used with packaging plasmids such as pCMV-dR8.2...

...developed split cytosine and adenine base editors that can be reconstituted in vivo to form a protein identical.... Broadening the spectral diversity of GEVIs can enable resolving single action potentials and further...allows for greater reproducibility as scientists can assemble parts from different labs and experimental...proteins controlled by Cre recombination. Each cell can have multiple copies of the transgene that recombine...

... acids, HA is nine), multiple repeats of the tag can recruit multiple frankenbodies to amplify the signal...two guides require two arrays for Cas9, two guides can be streamlined into a single array for Cas12a. Image...histidine tags, are optimized for SDS-PAGE gels and can be detected on Western blots via engineered immunoglobulin...editor allows for base editing, while gene editing can be done via a nuclease-active CasMINI. This small...is highly specific in mammalian cells without significant off-target effects, yet small enough to be packaged...

...mentoring program at your organization, contact me! I can provide a step-by-step, how-to guide to get one started...same time Broadens the network of the participants Can be a lot of fun! Form your own peer mentoring group...interested and willing to commit to the group. The group can be one gender and career stage (e.g. all women, all... mixed for one or both of these criteria. If you can, include people from different labs, departments ... from peer groups meeting at both the Fox Chase Cancer Center and Brandeis University. 5 easy steps to...meet regularly no matter what, even if one person can’t make it. One of the biggest group mentoring predictors...

... with the other 11 presents!)  Can't get enough of CRISPR? Neither can we! Subscribe below to get updates...CRISPR in 2021 and useful resources, fear not! You can find all of them here.  On the first day of CRISPR...

...interested in engaging with us on social media, you can find us on Bluesky: @addgene.bsky.social; or @addgene...associated with an official Addgene account. You can reach out to us directly via email at [email protected]... suggestions in the comments.  X has moved significantly away from the functionality and features that...this reason, our timeline has accelerated. We significantly reduced posting on November 20, 2023, and as...