...addition of MnCl2. Disadvantages of this method include favoring of certain types of mutations, difficulty...

...developed based on miRFP760-miRFP720 FRET pair, including Rac1 biosensor (Shcherbakova et al., 2018) and...

...variants deposited with Addgene. These variants, including a “miniCRISPR” version, are much smaller proteins...

...SDSA is a conservative repair mechanism which exclusively yields non-crossover events. This means all newly...

...two commonly employed methods for titering IDLVs include detecting vector particle components or performing...

...axis. This effect causes misleading qualitative conclusions, and it’s also problematic for computational ...

... sequencing functional regions of the plasmid including the insert. This is essential since mutations ...

...high-quality DNA, real-time PCR has several advantages including easier automation, higher throughput screening...

... genetic diseases caused by splicing defects, including ataxia telangiectasia, congenital disorder of ...

...and samples in duplicate at least If possible, include an AAV reference sample of known titer. The reference...expected titer of the samples being tested. Always include a No Template Control (NTC), i.e master mix + water... of each sample in duplicate. Do not forget to include a no template control ( NTC = master mix + water...dilution is appropriate). Quality of duplicates: Exclude duplicates from analyses if there is more than ...

...Other methods of generating a monoclonal cell line include single-cell sorting (Link opens in a new window... protocol will vary based on several factors, including the growth properties of the cell lines, the effects...cell pool by trypsinization and break up any cell clumps into individual cells by passing several times ...Wild-type A549 cells do not express Cas9 and are included as a control (WT). Vinculin is a loading control...

...of applications of the Enhanced Nano-Lanterns including monitoring multiple cellular events such as the...

...refer to the paper for more details on the assay, including examples of RBPs successfully identified using...

...plasmid sequencing services to verify the plasmids, including long-read sequencing techniques, such as Oxford...

... images once taken. To do so, you will need to include a marker like the Hoechst stain for the nuclei....

...recombinase-specific co-factors (which should be excluded from the system), these reactions are irreversible...

...targeting, which is limited to a few strains, including 129 and C57BL/6, for which we have germline competent...

...visualize the DNA in your gel (this table is not inclusive of all gel stains):  Stain Pre or Post Run?...

...helped assemble a crack team of Addgenies that includes: Michelle, the Senior Scientist who coordinates...

...Microbial rhodopsins are encoded by opsin genes, and include bacteriorhodopsins, halorhodopsins, and channelrhodopsins...