We narrowed to 799 results for: Clu

...smFPs can result in better resolution images. Conclusion By combining advantages of FPs and epitope tags...

...Get sponsors for a training weekend for a sports club, find speakers for a meetup or recruit volunteers...

...administered, which leads to many complications, including the inability of some institutions to know exactly...

...variants deposited with Addgene. These variants, including a “miniCRISPR” version, are much smaller proteins...

...sequence in a plasmid and call it a day. You need to include both sides of the complementary RNA duplex and ...

...two commonly employed methods for titering IDLVs include detecting vector particle components or performing...

...axis. This effect causes misleading qualitative conclusions, and it’s also problematic for computational ...

...SDSA is a conservative repair mechanism which exclusively yields non-crossover events. This means all newly...

...developed based on miRFP760-miRFP720 FRET pair, including Rac1 biosensor (Shcherbakova et al., 2018) and...

...addition of MnCl2. Disadvantages of this method include favoring of certain types of mutations, difficulty...

...past fifteen years. Some examples from their lab include using AAV to introduce epitope tags into the endogenous...

... sequencing functional regions of the plasmid including the insert. This is essential since mutations ...

...high-quality DNA, real-time PCR has several advantages including easier automation, higher throughput screening...

... genetic diseases caused by splicing defects, including ataxia telangiectasia, congenital disorder of ...

...and samples in duplicate at least If possible, include an AAV reference sample of known titer. The reference...expected titer of the samples being tested. Always include a No Template Control (NTC), i.e master mix + water... of each sample in duplicate. Do not forget to include a no template control ( NTC = master mix + water...dilution is appropriate). Quality of duplicates: Exclude duplicates from analyses if there is more than ...

... images once taken. To do so, you will need to include a marker like the Hoechst stain for the nuclei....

...plasmid sequencing services to verify the plasmids, including long-read sequencing techniques, such as Oxford...

...refer to the paper for more details on the assay, including examples of RBPs successfully identified using...

...recombinase-specific co-factors (which should be excluded from the system), these reactions are irreversible...

...targeting, which is limited to a few strains, including 129 and C57BL/6, for which we have germline competent...