We narrowed to 802 results for: Clu

1. Viral Vectors 101: Pseudotyping

   Type

   Blog Post

   ..., Bieniasz PD, Jäger U, Porter CD, Friedman T, McClure MO, Weiss RA (1997) Sensitization of rhabdo-, lenti...

2. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

   Type

   Blog Post

   ... from many with experience mentoring in STEM, including Addgene’s Joanne Kamens. Expanding Binning Singletons...

3. Neuronal labeling with Spaghetti Monster

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   Blog Post

   ...smFPs can result in better resolution images. Conclusion By combining advantages of FPs and epitope tags...

4. Transferable Skills Guide: External Collaborations

   Type

   Blog Post

   ...Get sponsors for a training weekend for a sports club, find speakers for a meetup or recruit volunteers...

5. Changing Labor Laws Bring Increased Postdoc Wages

   Type

   Blog Post

   ...administered, which leads to many complications, including the inability of some institutions to know exactly...

6. Deep Mutational Scanning with One Pot Saturation Mutagenesis

   Type

   Blog Post

   ...addition of MnCl2. Disadvantages of this method include favoring of certain types of mutations, difficulty...

7. Quick Guide to Near-Infrared Fluorescent Proteins

   Type

   Blog Post

   ...developed based on miRFP760-miRFP720 FRET pair, including Rac1 biosensor (Shcherbakova et al., 2018) and...

8. Plasmids 101: Expressing shRNAs for RNA Interference

   Type

   Blog Post

   ...sequence in a plasmid and call it a day. You need to include both sides of the complementary RNA duplex and ...

9. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

   Type

   Blog Post

   ...two commonly employed methods for titering IDLVs include detecting vector particle components or performing...

10. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type

    Blog Post

    ...axis. This effect causes misleading qualitative conclusions, and it’s also problematic for computational ...

11. Hot Plasmids: Summer 2024

    Type

    Blog Post

    ...variants deposited with Addgene. These variants, including a “miniCRISPR” version, are much smaller proteins...

12. CRISPR 101: Homology Directed Repair

    Type

    Blog Post

    ...SDSA is a conservative repair mechanism which exclusively yields non-crossover events. This means all newly...

13. Adeno-associated Viruses (AAVs) for Genome Editing

    Type

    Blog Post

    ...past fifteen years. Some examples from their lab include using AAV to introduce epitope tags into the endogenous...

14. Site Directed Mutagenesis by PCR

    Type

    Blog Post

    ... sequencing functional regions of the plasmid including the insert. This is essential since mutations ...

15. Evolution of Lab Techniques

    Type

    Blog Post

    ...high-quality DNA, real-time PCR has several advantages including easier automation, higher throughput screening...

16. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type

    Blog Post

    ... genetic diseases caused by splicing defects, including ataxia telangiectasia, congenital disorder of ...

17. AAV Titration by qPCR Using SYBR Green Technology

    Type

    Protocol

    ...and samples in duplicate at least If possible, include an AAV reference sample of known titer. The reference...expected titer of the samples being tested. Always include a No Template Control (NTC), i.e master mix + water... of each sample in duplicate. Do not forget to include a no template control ( NTC = master mix + water...dilution is appropriate). Quality of duplicates: Exclude duplicates from analyses if there is more than ...

18. Antibodies 101: Multiplex Immunofluorescence

    Type

    Blog Post

    ... images once taken. To do so, you will need to include a marker like the Hoechst stain for the nuclei....

19. Plasmids 101: Dimers and Multimers

    Type

    Blog Post

    ...plasmid sequencing services to verify the plasmids, including long-read sequencing techniques, such as Oxford...

20. Technologies Enabled by NanoLuc® Luciferase

    Type

    Blog Post

    ...of applications of the Enhanced Nano-Lanterns including monitoring multiple cellular events such as the...